Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   TBH_RS13420 Genome accession   NZ_AP012273
Coordinates   2826559..2827596 (-) Length   345 a.a.
NCBI ID   WP_041069233.1    Uniprot ID   A0A7U6GL41
Organism   Thiolapillus brandeum strain Hiromi     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2821559..2832596
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TBH_RS13395 (TBH_C2598) pyrR 2821626..2822165 (+) 540 WP_052470194.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  TBH_RS13400 (TBH_C2599) - 2822162..2823136 (+) 975 WP_041069221.1 aspartate carbamoyltransferase catalytic subunit -
  TBH_RS13405 (TBH_C2600) - 2823133..2824416 (+) 1284 WP_041069224.1 dihydroorotase -
  TBH_RS13410 (TBH_C2601) - 2824413..2825276 (+) 864 WP_041069227.1 dihydroorotate dehydrogenase electron transfer subunit -
  TBH_RS13415 (TBH_C2602) pilU 2825329..2826447 (-) 1119 WP_041069230.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  TBH_RS13420 (TBH_C2603) pilT 2826559..2827596 (-) 1038 WP_041069233.1 type IV pilus twitching motility protein PilT Machinery gene
  TBH_RS13425 (TBH_C2604) - 2827818..2828501 (+) 684 WP_041069236.1 YggS family pyridoxal phosphate-dependent enzyme -
  TBH_RS13430 (TBH_C2605) proC 2828498..2829328 (+) 831 WP_041069239.1 pyrroline-5-carboxylate reductase -
  TBH_RS13435 (TBH_C2606) - 2829328..2829906 (+) 579 WP_172649518.1 YggT family protein -
  TBH_RS13440 (TBH_C2607) - 2829903..2830187 (+) 285 WP_041069244.1 DUF167 domain-containing protein -
  TBH_RS13445 (TBH_C2608) - 2830359..2832332 (+) 1974 WP_082030759.1 dynamin family protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38195.79 Da        Isoelectric Point: 6.6626

>NTDB_id=65092 TBH_RS13420 WP_041069233.1 2826559..2827596(-) (pilT) [Thiolapillus brandeum strain Hiromi]
MDITELLAFSVKHNASDLHLSAGLPPMIRVDGDIRRINVPALDHKTVHSLVYDIMNDKQRRDYEEFMEVDFSFEIPGLAR
FRVNAFNQDRGAAAVFRTIPSKVLTLDDLGCPSTFKTICDAPRGIVLVTGPTGSGKSTTLAAMIDYINDNEYGHVLTIED
PIEFVHQSKKCLINQREVHRDTLGFSEALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRV
VDVFPAGEKSMVRSMLSESLRAVIAQTLLKKVGGGRTAAWEIMIGTPAIRNLIREDKVAQMYSAIQTGQAVGMQTLDQHL
KELLQKGLVTRQDARSRAQNKEAFT

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=65092 TBH_RS13420 WP_041069233.1 2826559..2827596(-) (pilT) [Thiolapillus brandeum strain Hiromi]
ATGGATATTACAGAGCTTCTTGCGTTCAGCGTAAAACACAACGCTTCTGATTTGCACCTGTCGGCGGGATTGCCCCCCAT
GATACGTGTCGATGGTGATATCCGGCGCATCAACGTGCCGGCGCTCGATCACAAGACCGTGCATTCGTTGGTGTACGACA
TCATGAATGACAAGCAGCGCCGGGACTATGAAGAATTCATGGAAGTGGATTTTTCTTTTGAAATTCCGGGGCTGGCGCGA
TTCAGGGTCAATGCCTTCAATCAGGATCGCGGCGCAGCAGCAGTATTCAGAACCATTCCGTCCAAGGTGTTGACCTTGGA
TGATCTGGGGTGCCCCTCTACTTTCAAGACCATCTGTGATGCGCCCCGTGGCATTGTGCTGGTAACCGGACCTACGGGTT
CTGGTAAATCCACTACCCTGGCCGCGATGATCGACTATATCAACGACAATGAGTACGGCCATGTGCTGACTATCGAAGAT
CCCATAGAATTCGTACACCAGAGCAAGAAATGCCTGATCAATCAGCGGGAAGTGCATCGTGATACCCTGGGTTTTTCCGA
AGCCCTGCGTTCCGCCCTTCGGGAAGACCCGGATATCATCCTGGTGGGTGAATTGCGGGATCTGGAAACCATCCGGCTGG
CCCTCACGGCTGCCGAGACCGGCCACCTGGTGTTCGGCACCCTGCATACCAGTTCCGCCGCCAAGACCATCGACCGTGTC
GTGGATGTATTCCCGGCGGGCGAAAAATCCATGGTGCGTTCCATGTTGTCAGAGTCCCTGAGGGCGGTTATCGCCCAGAC
CCTGCTGAAGAAAGTGGGAGGTGGGCGTACCGCAGCCTGGGAGATCATGATTGGAACCCCCGCGATCCGGAATCTGATTC
GCGAGGACAAGGTGGCGCAGATGTATTCCGCCATCCAGACCGGCCAGGCCGTGGGCATGCAGACTCTGGATCAGCATCTC
AAGGAGTTGCTGCAGAAGGGCCTTGTCACCCGTCAGGATGCCCGTTCCAGAGCGCAAAACAAGGAAGCCTTTACCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7U6GL41

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

81.395

99.71

0.812
  pilT Acinetobacter nosocomialis M2

80.814

99.71

0.806
  pilT Acinetobacter baumannii D1279779

80.814

99.71

0.806
  pilT Acinetobacter baumannii strain A118

80.814

99.71

0.806
  pilT Pseudomonas aeruginosa PAK

80.233

99.71

0.8
  pilT Acinetobacter baylyi ADP1

78.198

99.71

0.78
  pilT Legionella pneumophila strain Lp02

74.709

99.71

0.745
  pilT Legionella pneumophila strain ERS1305867

74.709

99.71

0.745
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.206

98.261

0.69
  pilT Vibrio cholerae strain A1552

70.206

98.261

0.69
  pilT Neisseria meningitidis 8013

67.63

100

0.678
  pilT Neisseria gonorrhoeae MS11

67.341

100

0.675
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.014

100

0.51
  pilU Pseudomonas stutzeri DSM 10701

41.791

97.101

0.406
  pilU Vibrio cholerae strain A1552

40.299

97.101

0.391
  pilU Acinetobacter baylyi ADP1

39.706

98.551

0.391

Multiple sequence alignment