Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   TBH_RS13415 Genome accession   NZ_AP012273
Coordinates   2825329..2826447 (-) Length   372 a.a.
NCBI ID   WP_041069230.1    Uniprot ID   -
Organism   Thiolapillus brandeum strain Hiromi     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2820329..2831447
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TBH_RS13385 (TBH_C2596) - 2820669..2821232 (+) 564 WP_041069215.1 YqgE/AlgH family protein -
  TBH_RS13390 (TBH_C2597) ruvX 2821225..2821629 (+) 405 WP_041069218.1 Holliday junction resolvase RuvX -
  TBH_RS13395 (TBH_C2598) pyrR 2821626..2822165 (+) 540 WP_052470194.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  TBH_RS13400 (TBH_C2599) - 2822162..2823136 (+) 975 WP_041069221.1 aspartate carbamoyltransferase catalytic subunit -
  TBH_RS13405 (TBH_C2600) - 2823133..2824416 (+) 1284 WP_041069224.1 dihydroorotase -
  TBH_RS13410 (TBH_C2601) - 2824413..2825276 (+) 864 WP_041069227.1 dihydroorotate dehydrogenase electron transfer subunit -
  TBH_RS13415 (TBH_C2602) pilU 2825329..2826447 (-) 1119 WP_041069230.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  TBH_RS13420 (TBH_C2603) pilT 2826559..2827596 (-) 1038 WP_041069233.1 type IV pilus twitching motility protein PilT Machinery gene
  TBH_RS13425 (TBH_C2604) - 2827818..2828501 (+) 684 WP_041069236.1 YggS family pyridoxal phosphate-dependent enzyme -
  TBH_RS13430 (TBH_C2605) proC 2828498..2829328 (+) 831 WP_041069239.1 pyrroline-5-carboxylate reductase -
  TBH_RS13435 (TBH_C2606) - 2829328..2829906 (+) 579 WP_172649518.1 YggT family protein -
  TBH_RS13440 (TBH_C2607) - 2829903..2830187 (+) 285 WP_041069244.1 DUF167 domain-containing protein -

Sequence

Protein


Download         Length: 372 a.a.        Molecular weight: 41493.94 Da        Isoelectric Point: 6.3745

>NTDB_id=65091 TBH_RS13415 WP_041069230.1 2825329..2826447(-) (pilU) [Thiolapillus brandeum strain Hiromi]
MDFNDILRLMADKKASDLFITVGRPPCVKVDGHIKPVAKTPLTADQSRELAYGLMSPEQREEFRHTKECNFGVSAPGIGR
FRVSAFVQRDAVAMVLRRIETDIPSFEDLKLPPIIKQLAMIKRGLIVFVGATGTGKSTSLAAMLGYRNRKSDGHIICIED
PIEFLHQHEGCIVAQREVGIDTESYEVALKNTLRQAPDVILIGEIRTKEVMEHALTFAETGHLVLTTLHANNANQALERI
LHFFPEERKEQALMDLSLNLKGILAQQLIPRADGVGRRAAIEVLLNTPLASDLILKGKIHELKELMKRSREHGMITFDQA
LYELYLEGEITEADALKHADSANEVRLMIKLGDNTPGSRARDLDAAMLVDDY

Nucleotide


Download         Length: 1119 bp        

>NTDB_id=65091 TBH_RS13415 WP_041069230.1 2825329..2826447(-) (pilU) [Thiolapillus brandeum strain Hiromi]
ATGGATTTCAATGACATACTCCGCCTGATGGCGGACAAGAAAGCATCCGATCTGTTTATCACTGTAGGACGCCCACCATG
TGTCAAAGTGGATGGTCATATCAAGCCTGTGGCCAAAACACCACTTACGGCAGATCAGAGTCGCGAGCTGGCCTATGGCT
TGATGTCCCCTGAGCAGCGGGAGGAGTTTCGTCATACCAAGGAATGCAACTTCGGTGTCAGCGCCCCGGGTATTGGTCGA
TTCCGCGTAAGCGCCTTTGTGCAGCGCGATGCCGTAGCCATGGTGCTGCGCCGTATCGAAACCGACATTCCCAGTTTCGA
GGATCTGAAGCTTCCTCCCATCATCAAGCAGCTGGCTATGATCAAGCGTGGATTGATTGTTTTCGTGGGTGCTACGGGCA
CGGGTAAATCCACTTCCCTGGCAGCCATGTTGGGTTACCGCAACCGAAAGAGTGACGGGCATATCATTTGTATCGAGGAT
CCCATCGAATTTCTTCACCAGCATGAAGGCTGCATTGTGGCTCAACGGGAAGTGGGCATTGATACCGAGTCTTACGAAGT
CGCCCTGAAGAATACTCTACGTCAGGCCCCGGATGTGATACTCATTGGCGAGATTCGCACCAAGGAAGTCATGGAACATG
CCCTGACTTTTGCCGAAACGGGCCATTTGGTGCTTACGACTCTGCATGCCAACAACGCCAACCAGGCCCTGGAGCGGATC
CTGCACTTCTTTCCCGAGGAGCGCAAGGAACAGGCTCTCATGGATTTGTCCCTGAACCTCAAGGGCATTCTCGCCCAGCA
GCTCATTCCCCGGGCCGATGGCGTGGGCCGCCGGGCCGCCATTGAAGTGTTACTGAATACGCCCTTGGCATCGGATCTGA
TTCTCAAGGGCAAGATCCACGAACTCAAGGAACTCATGAAGCGTTCCCGCGAGCATGGCATGATCACCTTCGACCAGGCT
CTGTACGAGCTCTACCTGGAAGGTGAGATCACTGAAGCAGATGCCCTCAAACATGCCGATTCCGCCAACGAAGTACGCCT
GATGATCAAGCTGGGAGACAACACTCCTGGCTCCCGGGCGCGGGATCTGGATGCCGCCATGCTGGTGGACGATTACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

69.188

95.968

0.664
  pilU Acinetobacter baylyi ADP1

64.345

96.505

0.621
  pilU Vibrio cholerae strain A1552

59.143

94.086

0.556
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.363

91.129

0.395
  pilT Legionella pneumophila strain Lp02

41.194

90.054

0.371
  pilT Legionella pneumophila strain ERS1305867

41.194

90.054

0.371

Multiple sequence alignment