Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   L0993_RS12305 Genome accession   NZ_CP090845
Coordinates   2785530..2786567 (-) Length   345 a.a.
NCBI ID   WP_017056522.1    Uniprot ID   -
Organism   Vibrio kanaloae strain WXL161     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2780530..2791567
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L0993_RS12280 (L0993_12270) rsmE 2781249..2781980 (+) 732 WP_077680890.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  L0993_RS12285 (L0993_12275) gshB 2781990..2782940 (+) 951 WP_077680891.1 glutathione synthase -
  L0993_RS12290 (L0993_12280) - 2783047..2783610 (+) 564 WP_017056525.1 YqgE/AlgH family protein -
  L0993_RS12295 (L0993_12285) ruvX 2783663..2784085 (+) 423 WP_029626997.1 Holliday junction resolvase RuvX -
  L0993_RS12300 (L0993_12290) pilU 2784405..2785514 (-) 1110 WP_046225188.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  L0993_RS12305 (L0993_12295) pilT 2785530..2786567 (-) 1038 WP_017056522.1 type IV pilus twitching motility protein PilT Machinery gene
  L0993_RS12310 (L0993_12300) - 2786594..2787298 (+) 705 WP_017056521.1 YggS family pyridoxal phosphate-dependent enzyme -
  L0993_RS12315 (L0993_12305) proC 2787381..2788199 (+) 819 WP_046225187.1 pyrroline-5-carboxylate reductase -
  L0993_RS12320 (L0993_12310) - 2788277..2788834 (+) 558 WP_017067798.1 YggT family protein -
  L0993_RS12325 (L0993_12315) yggU 2788864..2789154 (+) 291 WP_017056518.1 DUF167 family protein YggU -
  L0993_RS12330 (L0993_12320) - 2789183..2789614 (+) 432 WP_017056517.1 DUF4426 domain-containing protein -
  L0993_RS12335 (L0993_12325) - 2789662..2790261 (+) 600 WP_373937810.1 XTP/dITP diphosphatase -
  L0993_RS12340 (L0993_12330) hemW 2790261..2791448 (+) 1188 WP_136981426.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38239.75 Da        Isoelectric Point: 6.3185

>NTDB_id=645180 L0993_RS12305 WP_017056522.1 2785530..2786567(-) (pilT) [Vibrio kanaloae strain WXL161]
MDITELLDFSVKHNASDLHLSAGVSPMVRIDGEVRKLGVPALSHADVHRLVFEIMNDSQRGEFEEKLEVDFSFELPNVGR
FRVNAFNQARGCSAVFRSIPVEIPTLEQLGAPEIFEKIANYEKGLVLVTGPTGSGKSTTLAAMVDYVNRNHNKHILTIED
PIEFVHTNNKCLVNQREVHRDTHSFKAALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKSMVRSMLSESLRSVIAQKLLKRVGGGRVACHEIMMATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNA
KQLMAQGLVDSEEVEKKIEIETSRF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=645180 L0993_RS12305 WP_017056522.1 2785530..2786567(-) (pilT) [Vibrio kanaloae strain WXL161]
ATGGATATCACCGAGTTACTAGATTTTAGTGTAAAGCATAACGCATCAGATCTACATCTTTCTGCGGGTGTATCTCCAAT
GGTACGTATAGATGGTGAAGTAAGAAAGCTTGGAGTGCCCGCTTTGAGTCATGCAGATGTGCATCGTTTAGTTTTTGAGA
TCATGAACGATTCACAGCGCGGTGAGTTTGAAGAGAAACTCGAAGTCGACTTCTCTTTTGAATTACCCAATGTTGGTCGT
TTCCGTGTGAATGCTTTTAATCAAGCTCGTGGTTGCTCAGCTGTGTTTCGATCTATCCCTGTAGAGATCCCAACTTTAGA
GCAGTTAGGCGCGCCTGAAATCTTTGAAAAGATTGCTAACTACGAAAAAGGTTTAGTGCTGGTCACTGGCCCAACTGGTT
CTGGTAAATCGACGACTCTTGCGGCGATGGTTGATTATGTTAACCGTAACCATAACAAGCACATCCTGACGATCGAAGAC
CCGATTGAATTCGTTCATACCAACAATAAATGCCTCGTTAACCAGCGAGAAGTTCATCGTGATACCCACAGCTTTAAAGC
GGCGCTACGCAGTGCATTGCGCGAAGACCCTGACGTTATCCTTGTGGGTGAGCTTCGTGACCAAGAGACGATCAGCTTAG
CGCTAACGGCCGCAGAAACAGGCCATCTAGTTTTTGGTACTCTGCACACCAGTTCTGCTGCTAAAACCATAGATCGCATT
ATTGATGTGTTCCCCGGCAGCGACAAATCTATGGTTCGTTCAATGCTATCCGAATCACTACGCTCGGTGATTGCTCAAAA
ATTGTTAAAGCGTGTAGGCGGTGGTCGTGTGGCTTGTCATGAAATTATGATGGCGACACCTGCAATTAGAAACTTGATTC
GTGAAGACAAAGTTGCACAGATGTACTCGATTATTCAAACTGGCGCAGCACATGGTATGCAAACCATGGAGCAGAATGCG
AAGCAGCTTATGGCCCAAGGTTTAGTTGATTCAGAAGAAGTCGAGAAGAAGATTGAAATCGAAACCTCGCGGTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

89.275

100

0.893
  pilT Vibrio cholerae strain A1552

89.275

100

0.893
  pilT Acinetobacter baumannii D1279779

71.988

96.232

0.693
  pilT Acinetobacter baumannii strain A118

71.988

96.232

0.693
  pilT Acinetobacter nosocomialis M2

71.687

96.232

0.69
  pilT Pseudomonas stutzeri DSM 10701

70.03

97.681

0.684
  pilT Pseudomonas aeruginosa PAK

69.118

98.551

0.681
  pilT Acinetobacter baylyi ADP1

70.909

95.652

0.678
  pilT Legionella pneumophila strain Lp02

68.389

95.362

0.652
  pilT Legionella pneumophila strain ERS1305867

68.389

95.362

0.652
  pilT Neisseria meningitidis 8013

63.235

98.551

0.623
  pilT Neisseria gonorrhoeae MS11

62.941

98.551

0.62
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.44

98.841

0.499
  pilU Vibrio cholerae strain A1552

43.385

94.203

0.409
  pilU Pseudomonas stutzeri DSM 10701

41.317

96.812

0.4
  pilU Acinetobacter baylyi ADP1

39.688

92.754

0.368