Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   L0993_RS12300 Genome accession   NZ_CP090845
Coordinates   2784405..2785514 (-) Length   369 a.a.
NCBI ID   WP_046225188.1    Uniprot ID   -
Organism   Vibrio kanaloae strain WXL161     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2779405..2790514
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L0993_RS12270 (L0993_12260) - 2779775..2780275 (+) 501 WP_017056529.1 SprT family zinc-dependent metalloprotease -
  L0993_RS12275 (L0993_12265) - 2780372..2781103 (+) 732 WP_017056528.1 endonuclease -
  L0993_RS12280 (L0993_12270) rsmE 2781249..2781980 (+) 732 WP_077680890.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  L0993_RS12285 (L0993_12275) gshB 2781990..2782940 (+) 951 WP_077680891.1 glutathione synthase -
  L0993_RS12290 (L0993_12280) - 2783047..2783610 (+) 564 WP_017056525.1 YqgE/AlgH family protein -
  L0993_RS12295 (L0993_12285) ruvX 2783663..2784085 (+) 423 WP_029626997.1 Holliday junction resolvase RuvX -
  L0993_RS12300 (L0993_12290) pilU 2784405..2785514 (-) 1110 WP_046225188.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  L0993_RS12305 (L0993_12295) pilT 2785530..2786567 (-) 1038 WP_017056522.1 type IV pilus twitching motility protein PilT Machinery gene
  L0993_RS12310 (L0993_12300) - 2786594..2787298 (+) 705 WP_017056521.1 YggS family pyridoxal phosphate-dependent enzyme -
  L0993_RS12315 (L0993_12305) proC 2787381..2788199 (+) 819 WP_046225187.1 pyrroline-5-carboxylate reductase -
  L0993_RS12320 (L0993_12310) - 2788277..2788834 (+) 558 WP_017067798.1 YggT family protein -
  L0993_RS12325 (L0993_12315) yggU 2788864..2789154 (+) 291 WP_017056518.1 DUF167 family protein YggU -
  L0993_RS12330 (L0993_12320) - 2789183..2789614 (+) 432 WP_017056517.1 DUF4426 domain-containing protein -
  L0993_RS12335 (L0993_12325) - 2789662..2790261 (+) 600 WP_373937810.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41384.28 Da        Isoelectric Point: 6.0667

>NTDB_id=645179 L0993_RS12300 WP_046225188.1 2784405..2785514(-) (pilU) [Vibrio kanaloae strain WXL161]
MELNQILEGMLSQKASDLYITVDAPVLFRVDGELRPQGEKLNTAQVSQLLDAMMEQDRRDEYQQTREANFAIVRDIGRFR
VSAFFQRELPGAVIRRIETNIPTFEQLKLPDVLQDLSIAKRGLVLVVGATGSGKSTSMAAMTGYRNANRSGHILTIEDPI
EFVHEHRKCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKEQKEQFLFDLSMNLRGVVAQQLIRDKNANGRHGVFEVLLNSPRVSDLIRRGELHELKSTMAKSKEIGMQTFDQALY
DLVVAGKISEEDAFHSADSANDLRLMLKTKRGDNDNGIGALSSVKIDMG

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=645179 L0993_RS12300 WP_046225188.1 2784405..2785514(-) (pilU) [Vibrio kanaloae strain WXL161]
ATGGAGTTGAATCAAATTCTTGAAGGCATGCTTTCACAGAAAGCGTCGGATCTTTACATCACCGTGGACGCACCAGTTTT
GTTTCGTGTTGATGGTGAACTGCGGCCTCAGGGAGAAAAGCTGAATACGGCTCAGGTCTCTCAATTGCTTGATGCGATGA
TGGAGCAAGATCGACGCGATGAATACCAACAAACACGCGAGGCTAATTTTGCGATTGTGCGTGATATTGGCCGTTTTCGT
GTGAGTGCTTTCTTCCAACGAGAGCTTCCTGGTGCGGTAATTAGACGCATCGAGACTAACATTCCAACCTTTGAACAATT
AAAGCTTCCTGATGTGCTACAAGACCTTTCAATAGCGAAACGCGGACTTGTGCTAGTGGTTGGAGCGACGGGGTCTGGTA
AATCAACTTCAATGGCTGCGATGACAGGGTACCGGAATGCTAACCGTTCTGGTCATATTTTGACGATTGAAGATCCGATT
GAATTCGTGCATGAACATAGAAAGTGCATTGTTACTCAGCGTGAGGTCGGACTCGACACTGAGAGTTATGAAGTCGCGCT
TAAGAACTCCCTGCGCCAAGCTCCCGACATGATCTTGATTGGTGAGATCCGTAGCCGTGAAACCATGGAGTACGCGATGA
CTTTTGCTGAGACTGGTCACCTGTGTATGGCAACTCTGCATGCAAATAATGCTAATCAAGCACTAGAACGTATTCTGCAT
TTGGTTCCCAAAGAGCAAAAAGAGCAATTCTTGTTTGATCTGTCGATGAACTTACGTGGTGTGGTGGCACAGCAATTAAT
TCGAGACAAGAATGCCAATGGTCGTCATGGGGTGTTCGAGGTTCTTTTGAACAGCCCGCGAGTGTCGGACCTGATTCGTC
GTGGTGAGTTGCATGAACTCAAATCGACAATGGCTAAATCGAAGGAGATTGGGATGCAGACCTTTGACCAAGCTTTGTAT
GATTTAGTCGTTGCGGGCAAAATCAGCGAAGAAGATGCGTTTCATAGTGCCGACTCTGCTAATGATCTGCGCTTAATGTT
GAAAACCAAGCGAGGTGATAATGACAATGGCATAGGAGCCCTCAGCAGTGTGAAAATCGATATGGGTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(6-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

83.424

99.729

0.832
  pilU Pseudomonas stutzeri DSM 10701

59.143

94.851

0.561
  pilU Acinetobacter baylyi ADP1

54.416

95.122

0.518
  pilT Legionella pneumophila strain ERS1305867

41.593

91.87

0.382
  pilT Legionella pneumophila strain Lp02

41.593

91.87

0.382
  pilT Pseudomonas aeruginosa PAK

41.493

90.786

0.377
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.284

87.805

0.371
  pilT Acinetobacter baumannii strain A118

40.413

91.87

0.371
  pilT Acinetobacter baumannii D1279779

40.413

91.87

0.371
  pilT Acinetobacter nosocomialis M2

40.413

91.87

0.371
  pilT Vibrio cholerae strain A1552

40.719

90.515

0.369
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.719

90.515

0.369
  pilT Pseudomonas stutzeri DSM 10701

40

90.786

0.363
  pilT Neisseria gonorrhoeae MS11

38.551

93.496

0.36
  pilT Neisseria meningitidis 8013

38.551

93.496

0.36
  pilT Acinetobacter baylyi ADP1

40.181

89.702

0.36