Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   LZI70_RS09155 Genome accession   NZ_CP090614
Coordinates   2028779..2029888 (+) Length   369 a.a.
NCBI ID   WP_255229875.1    Uniprot ID   -
Organism   Vibrio pelagius strain WXL662     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2023779..2034888
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LZI70_RS09120 (LZI70_09105) - 2023944..2024543 (-) 600 WP_239841281.1 XTP/dITP diphosphatase -
  LZI70_RS09125 (LZI70_09110) - 2024757..2025188 (-) 432 WP_255229869.1 DUF4426 domain-containing protein -
  LZI70_RS09130 (LZI70_09115) yggU 2025217..2025507 (-) 291 WP_255229870.1 DUF167 family protein YggU -
  LZI70_RS09135 (LZI70_09120) - 2025507..2026064 (-) 558 WP_255229871.1 YggT family protein -
  LZI70_RS09140 (LZI70_09125) proC 2026119..2026937 (-) 819 WP_255229872.1 pyrroline-5-carboxylate reductase -
  LZI70_RS09145 (LZI70_09130) - 2026995..2027699 (-) 705 WP_255229873.1 YggS family pyridoxal phosphate-dependent enzyme -
  LZI70_RS09150 (LZI70_09135) pilT 2027726..2028763 (+) 1038 WP_255229874.1 type IV pilus twitching motility protein PilT Machinery gene
  LZI70_RS09155 (LZI70_09140) pilU 2028779..2029888 (+) 1110 WP_255229875.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LZI70_RS09160 (LZI70_09145) - 2029944..2030102 (-) 159 WP_255229876.1 hypothetical protein -
  LZI70_RS09165 (LZI70_09150) ruvX 2030198..2030620 (-) 423 WP_255229877.1 Holliday junction resolvase RuvX -
  LZI70_RS09170 (LZI70_09155) - 2030652..2031215 (-) 564 WP_032549843.1 YqgE/AlgH family protein -
  LZI70_RS09175 (LZI70_09160) gshB 2031309..2032259 (-) 951 WP_255229878.1 glutathione synthase -
  LZI70_RS09180 (LZI70_09165) rsmE 2032273..2033004 (-) 732 WP_255229879.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  LZI70_RS09185 (LZI70_09170) - 2033153..2033884 (-) 732 WP_255229880.1 endonuclease -
  LZI70_RS09190 (LZI70_09175) - 2033975..2034478 (-) 504 WP_255229881.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41289.11 Da        Isoelectric Point: 6.0181

>NTDB_id=644000 LZI70_RS09155 WP_255229875.1 2028779..2029888(+) (pilU) [Vibrio pelagius strain WXL662]
MELNQILEGMLSQKASDLYITVDAPVLFRVDGELRPQGEVLNAAQVSQLLDAMMDQERRDEYRQTREANFAIVRDSGRFR
VSAFFQRELPGAVIRRIETNIPTFEQLKLPDVLQDLSIAKRGLVLVVGATGSGKSTSMAAMTGYRNHNRSGHILTVEDPI
EFVHEHKKCIVTQREVGLDTESYEIALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKEQKEQFLFDMSMNLRGVVAQQLIRDKNGNGRHGVFEILLNSPRVSDLIRRGDLHELKATMAKSKEIGMQTFDQALY
DLVVAGKISEEDAFHSADSANDLRLMLKTQRGDGDYGSGSLAGVKIDMG

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=644000 LZI70_RS09155 WP_255229875.1 2028779..2029888(+) (pilU) [Vibrio pelagius strain WXL662]
ATGGAATTGAACCAGATCCTCGAGGGAATGCTGTCGCAAAAGGCGTCCGATCTTTATATCACGGTTGACGCGCCAGTGTT
GTTTCGCGTAGATGGGGAACTGCGTCCGCAAGGTGAAGTTCTCAATGCTGCTCAAGTGTCACAGCTTCTAGACGCGATGA
TGGATCAAGAGCGACGTGACGAGTACCGTCAAACACGCGAGGCGAACTTTGCGATTGTTCGAGATAGTGGTCGCTTTCGT
GTCAGTGCCTTCTTCCAGAGGGAACTGCCAGGTGCTGTTATTCGTCGTATTGAAACTAATATCCCAACCTTTGAGCAGTT
AAAGCTCCCGGATGTTTTACAAGACCTCTCAATCGCTAAGCGCGGCCTTGTGCTGGTGGTTGGTGCAACAGGTTCTGGTA
AGTCGACGTCGATGGCAGCGATGACGGGTTATCGCAACCACAATCGCTCAGGTCATATTCTTACGGTGGAAGACCCGATT
GAGTTTGTGCATGAGCACAAGAAGTGCATTGTCACCCAGCGCGAGGTGGGTTTAGATACAGAGAGCTACGAAATTGCGCT
TAAGAATTCGTTACGCCAAGCACCCGATATGATCTTGATTGGTGAGATTCGAAGCCGTGAAACCATGGAATACGCAATGA
CCTTTGCTGAAACGGGGCATCTGTGTATGGCAACGCTGCACGCAAATAATGCCAACCAAGCGTTAGAACGTATTCTTCAT
TTGGTGCCGAAAGAGCAAAAAGAGCAGTTCTTGTTTGATATGTCGATGAACCTACGTGGCGTTGTGGCGCAGCAGCTCAT
ACGAGACAAAAATGGCAATGGTCGTCATGGTGTGTTTGAGATCTTACTCAACAGCCCACGTGTGTCAGATCTTATTCGTC
GTGGTGATTTACATGAGCTCAAAGCAACGATGGCAAAGTCGAAAGAGATTGGAATGCAGACTTTCGATCAAGCATTGTAT
GACTTAGTTGTTGCAGGGAAAATCAGTGAAGAGGATGCATTCCACAGTGCTGACTCGGCGAACGATCTCAGGTTAATGTT
AAAAACGCAGCGCGGTGACGGTGATTACGGCAGTGGTTCTCTGGCCGGCGTGAAAATTGATATGGGTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(6-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

84.511

99.729

0.843
  pilU Pseudomonas stutzeri DSM 10701

55.526

100

0.558
  pilU Acinetobacter baylyi ADP1

53.561

95.122

0.509
  pilT Acinetobacter baumannii strain A118

42.059

92.141

0.388
  pilT Acinetobacter baumannii D1279779

42.059

92.141

0.388
  pilT Acinetobacter nosocomialis M2

42.059

92.141

0.388
  pilT Legionella pneumophila strain ERS1305867

41.888

91.87

0.385
  pilT Legionella pneumophila strain Lp02

41.888

91.87

0.385
  pilT Acinetobacter baylyi ADP1

41.52

92.683

0.385
  pilT Pseudomonas aeruginosa PAK

41.298

91.87

0.379
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.593

87.805

0.374
  pilT Pseudomonas stutzeri DSM 10701

39.823

91.87

0.366
  pilT Neisseria gonorrhoeae MS11

38.506

94.309

0.363
  pilT Neisseria meningitidis 8013

38.506

94.309

0.363
  pilT Vibrio cholerae strain A1552

40.12

90.515

0.363
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.12

90.515

0.363