Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LZI70_RS09150 Genome accession   NZ_CP090614
Coordinates   2027726..2028763 (+) Length   345 a.a.
NCBI ID   WP_255229874.1    Uniprot ID   -
Organism   Vibrio pelagius strain WXL662     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2022726..2033763
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LZI70_RS09115 (LZI70_09100) hemW 2022757..2023944 (-) 1188 WP_255229868.1 radical SAM family heme chaperone HemW -
  LZI70_RS09120 (LZI70_09105) - 2023944..2024543 (-) 600 WP_239841281.1 XTP/dITP diphosphatase -
  LZI70_RS09125 (LZI70_09110) - 2024757..2025188 (-) 432 WP_255229869.1 DUF4426 domain-containing protein -
  LZI70_RS09130 (LZI70_09115) yggU 2025217..2025507 (-) 291 WP_255229870.1 DUF167 family protein YggU -
  LZI70_RS09135 (LZI70_09120) - 2025507..2026064 (-) 558 WP_255229871.1 YggT family protein -
  LZI70_RS09140 (LZI70_09125) proC 2026119..2026937 (-) 819 WP_255229872.1 pyrroline-5-carboxylate reductase -
  LZI70_RS09145 (LZI70_09130) - 2026995..2027699 (-) 705 WP_255229873.1 YggS family pyridoxal phosphate-dependent enzyme -
  LZI70_RS09150 (LZI70_09135) pilT 2027726..2028763 (+) 1038 WP_255229874.1 type IV pilus twitching motility protein PilT Machinery gene
  LZI70_RS09155 (LZI70_09140) pilU 2028779..2029888 (+) 1110 WP_255229875.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LZI70_RS09160 (LZI70_09145) - 2029944..2030102 (-) 159 WP_255229876.1 hypothetical protein -
  LZI70_RS09165 (LZI70_09150) ruvX 2030198..2030620 (-) 423 WP_255229877.1 Holliday junction resolvase RuvX -
  LZI70_RS09170 (LZI70_09155) - 2030652..2031215 (-) 564 WP_032549843.1 YqgE/AlgH family protein -
  LZI70_RS09175 (LZI70_09160) gshB 2031309..2032259 (-) 951 WP_255229878.1 glutathione synthase -
  LZI70_RS09180 (LZI70_09165) rsmE 2032273..2033004 (-) 732 WP_255229879.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38191.64 Da        Isoelectric Point: 6.0485

>NTDB_id=643999 LZI70_RS09150 WP_255229874.1 2027726..2028763(+) (pilT) [Vibrio pelagius strain WXL662]
MDITELLDFSVKHNASDLHLSAGVSPMVRIDGEVRKLGIPALSHADVHRLVFEIMNDSQRSEFEEKLEVDFSFELPNVGR
FRVNAFNQSRGCSAVFRTIPVEIPTLEQLGAPDIFEKIANFEKGLVLVTGPTGSGKSTTLAAMVDYVNRNHNKHILTIED
PIEFVHTNNKCLVNQREVHRDTHSFKAALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLRSVIAQKLLKRVGGGRVACHEIMMATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNA
KQLIAQGVVDSDEVQNKIEIETALF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=643999 LZI70_RS09150 WP_255229874.1 2027726..2028763(+) (pilT) [Vibrio pelagius strain WXL662]
ATGGATATCACTGAGTTACTGGATTTTAGTGTAAAGCATAACGCGTCAGATCTACATCTTTCTGCGGGTGTATCTCCAAT
GGTACGTATAGATGGTGAAGTTAGGAAGCTTGGAATACCGGCTTTGAGCCATGCTGATGTCCATCGTTTGGTTTTTGAGA
TCATGAACGATTCACAGCGCAGTGAGTTTGAAGAGAAGCTGGAAGTCGACTTTTCATTTGAGTTGCCGAATGTTGGCCGT
TTTCGTGTGAATGCATTTAACCAGTCGCGTGGTTGTTCTGCGGTGTTTCGAACCATCCCGGTTGAGATCCCAACACTCGA
GCAGTTAGGTGCTCCTGATATCTTTGAAAAGATTGCTAACTTTGAGAAGGGCTTGGTTTTAGTCACTGGCCCAACGGGTT
CAGGTAAATCGACGACACTGGCGGCGATGGTTGATTATGTAAACCGTAACCACAATAAACACATCCTTACTATTGAAGAC
CCGATCGAATTTGTTCATACCAACAATAAGTGTCTTGTGAACCAGCGTGAGGTTCATCGTGATACTCACAGCTTTAAAGC
GGCGTTGCGCAGTGCACTGCGTGAAGACCCAGACGTGATTTTAGTGGGAGAGTTACGTGACCAAGAGACGATCAGCTTAG
CTTTAACTGCAGCTGAAACCGGCCACCTGGTGTTTGGTACGCTACATACTAGCTCTGCGGCTAAAACCATTGACCGTATT
ATTGATGTGTTTCCAGGCAGTGATAAGGACATGGTGCGCTCAATGCTGTCTGAGTCATTGCGCTCGGTGATTGCTCAGAA
GCTACTGAAGCGAGTAGGCGGAGGTCGTGTCGCTTGTCATGAGATTATGATGGCAACACCAGCTATTCGTAACTTGATTC
GTGAGGATAAAGTCGCTCAGATGTATTCCATCATTCAAACGGGGGCTGCACATGGCATGCAAACAATGGAGCAAAATGCC
AAACAGTTGATTGCTCAAGGCGTGGTGGACTCAGATGAAGTCCAAAACAAAATTGAAATCGAAACAGCACTGTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

89.855

100

0.899
  pilT Vibrio cholerae strain A1552

89.855

100

0.899
  pilT Acinetobacter baumannii D1279779

73.252

95.362

0.699
  pilT Acinetobacter baumannii strain A118

73.252

95.362

0.699
  pilT Acinetobacter nosocomialis M2

72.948

95.362

0.696
  pilT Pseudomonas stutzeri DSM 10701

69.733

97.681

0.681
  pilT Acinetobacter baylyi ADP1

71.212

95.652

0.681
  pilT Pseudomonas aeruginosa PAK

68.529

98.551

0.675
  pilT Legionella pneumophila strain Lp02

68.997

95.362

0.658
  pilT Legionella pneumophila strain ERS1305867

68.997

95.362

0.658
  pilT Neisseria meningitidis 8013

64.848

95.652

0.62
  pilT Neisseria gonorrhoeae MS11

62.647

98.551

0.617
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.898

96.812

0.493
  pilU Vibrio cholerae strain A1552

43.077

94.203

0.406
  pilU Pseudomonas stutzeri DSM 10701

41.317

96.812

0.4
  pilU Acinetobacter baylyi ADP1

39.688

92.754

0.368