Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   L0Y14_RS14000 Genome accession   NZ_CP090569
Coordinates   3105774..3106808 (-) Length   344 a.a.
NCBI ID   WP_005959900.1    Uniprot ID   -
Organism   Candidatus Endoriftia persephone isolate Tica-EPR-9o50.N     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3100774..3111808
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L0Y14_RS13980 (L0Y14_13960) - 3102987..3103316 (+) 330 WP_006474743.1 DUF1820 family protein -
  L0Y14_RS13985 (L0Y14_13965) - 3103408..3103965 (+) 558 WP_006474742.1 ankyrin repeat domain-containing protein -
  L0Y14_RS13990 (L0Y14_13970) - 3104071..3104529 (+) 459 WP_006474741.1 hypothetical protein -
  L0Y14_RS13995 (L0Y14_13975) pilU 3104546..3105673 (-) 1128 WP_006474740.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  L0Y14_RS14000 (L0Y14_13980) pilT 3105774..3106808 (-) 1035 WP_005959900.1 type IV pilus twitching motility protein PilT Machinery gene
  L0Y14_RS14005 (L0Y14_13985) - 3106972..3107661 (+) 690 WP_005959899.1 YggS family pyridoxal phosphate-dependent enzyme -
  L0Y14_RS14010 (L0Y14_13990) proC 3107776..3108603 (+) 828 WP_039959707.1 pyrroline-5-carboxylate reductase -
  L0Y14_RS14015 (L0Y14_13995) - 3108600..3109178 (+) 579 WP_005959897.1 YggT family protein -
  L0Y14_RS14020 (L0Y14_14000) - 3109178..3109468 (+) 291 WP_005959896.1 DUF167 family protein -
  L0Y14_RS14025 (L0Y14_14005) - 3109573..3111552 (+) 1980 WP_006474738.1 dynamin family protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37781.58 Da        Isoelectric Point: 6.5933

>NTDB_id=643542 L0Y14_RS14000 WP_005959900.1 3105774..3106808(-) (pilT) [Candidatus Endoriftia persephone isolate Tica-EPR-9o50.N]
MDIAELLAFSVKHSASDLHLSAGLPPMIRVDGDIRRINVPALEHKMVHGLVYDIMNDKQRKDFEEFLETDFSFEIPGLAR
FRVNAFNQARGASAVFRTIPSKVLTLEDLGCPGIFKDIVDVPRGLVLVTGPTGSGKSTTLAGMIDYKNDNDYAHILTIED
PIEFVHASKKCLINQREVHRDTLGFSEALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRV
IDVFPAGEKAMVRSMLSESLRAVIAQTLLKKIGGGRTAAWEIMVGTSAIRNLIREDKVAQMYSAIQTGQAAGMQTMDQAL
KELVEKGVISRLDAKAKAQNKDLF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=643542 L0Y14_RS14000 WP_005959900.1 3105774..3106808(-) (pilT) [Candidatus Endoriftia persephone isolate Tica-EPR-9o50.N]
ATGGATATAGCTGAATTGCTGGCTTTCAGTGTCAAACATAGCGCGTCGGATCTGCATCTGTCAGCCGGATTGCCGCCAAT
GATCCGGGTCGACGGCGATATTCGCCGCATCAACGTCCCAGCGTTGGAACACAAGATGGTTCACGGGCTTGTCTACGACA
TTATGAACGACAAGCAGCGCAAGGACTTTGAAGAGTTTCTCGAGACCGATTTCTCGTTCGAGATTCCCGGTCTGGCGCGT
TTTCGTGTCAACGCCTTCAACCAGGCGCGTGGCGCCTCCGCGGTGTTTCGTACCATCCCGTCGAAGGTGCTGACGTTGGA
GGATCTGGGCTGTCCGGGGATCTTCAAGGATATCGTCGATGTACCGCGCGGCCTGGTGTTGGTGACCGGCCCCACTGGTT
CAGGTAAGTCGACCACCCTGGCGGGCATGATCGACTACAAAAACGACAACGACTATGCGCATATCCTCACCATCGAGGAT
CCAATTGAGTTTGTCCACGCCAGCAAGAAGTGCCTGATTAACCAGCGCGAGGTGCACCGTGACACCCTCGGCTTCAGCGA
GGCGCTGCGCTCGGCGCTGCGTGAAGATCCGGATATCATCCTGGTCGGTGAGCTGCGTGACCTGGAGACCATCCGCCTAG
CGTTGACCGCTGCCGAGACCGGTCATCTGGTATTTGGCACCCTGCACACCAGTTCTGCCGCCAAGACTATTGACCGCGTG
ATCGATGTGTTTCCCGCCGGTGAGAAGGCGATGGTGCGCTCGATGCTGTCGGAGTCGCTGCGCGCAGTTATCGCCCAGAC
CCTGCTGAAGAAGATCGGTGGCGGCCGTACCGCCGCCTGGGAGATCATGGTCGGCACCTCGGCGATTCGTAACCTGATCC
GCGAAGATAAGGTGGCGCAGATGTACTCGGCGATCCAGACTGGCCAGGCGGCTGGTATGCAGACCATGGACCAGGCCCTC
AAGGAGCTGGTTGAGAAGGGCGTGATCAGTCGCCTCGATGCCAAGGCCAAGGCGCAGAACAAGGATCTGTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

81.977

100

0.82
  pilT Pseudomonas stutzeri DSM 10701

81.395

100

0.814
  pilT Acinetobacter baumannii D1279779

80.233

100

0.802
  pilT Acinetobacter baumannii strain A118

80.233

100

0.802
  pilT Acinetobacter nosocomialis M2

80.233

100

0.802
  pilT Acinetobacter baylyi ADP1

77.326

100

0.773
  pilT Legionella pneumophila strain Lp02

76.163

100

0.762
  pilT Legionella pneumophila strain ERS1305867

76.163

100

0.762
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.091

98.547

0.701
  pilT Vibrio cholerae strain A1552

71.091

98.547

0.701
  pilT Neisseria meningitidis 8013

66.472

99.709

0.663
  pilT Neisseria gonorrhoeae MS11

66.181

99.709

0.66
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.36

96.221

0.494
  pilU Pseudomonas stutzeri DSM 10701

42.09

97.384

0.41
  pilU Vibrio cholerae strain A1552

40.896

97.384

0.398
  pilU Acinetobacter baylyi ADP1

40.476

97.674

0.395