Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   L0Y14_RS13995 Genome accession   NZ_CP090569
Coordinates   3104546..3105673 (-) Length   375 a.a.
NCBI ID   WP_006474740.1    Uniprot ID   G2FFI0
Organism   Candidatus Endoriftia persephone isolate Tica-EPR-9o50.N     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3099546..3110673
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L0Y14_RS13975 (L0Y14_13955) recC 3099657..3102890 (-) 3234 WP_251859218.1 exodeoxyribonuclease V subunit gamma -
  L0Y14_RS13980 (L0Y14_13960) - 3102987..3103316 (+) 330 WP_006474743.1 DUF1820 family protein -
  L0Y14_RS13985 (L0Y14_13965) - 3103408..3103965 (+) 558 WP_006474742.1 ankyrin repeat domain-containing protein -
  L0Y14_RS13990 (L0Y14_13970) - 3104071..3104529 (+) 459 WP_006474741.1 hypothetical protein -
  L0Y14_RS13995 (L0Y14_13975) pilU 3104546..3105673 (-) 1128 WP_006474740.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  L0Y14_RS14000 (L0Y14_13980) pilT 3105774..3106808 (-) 1035 WP_005959900.1 type IV pilus twitching motility protein PilT Machinery gene
  L0Y14_RS14005 (L0Y14_13985) - 3106972..3107661 (+) 690 WP_005959899.1 YggS family pyridoxal phosphate-dependent enzyme -
  L0Y14_RS14010 (L0Y14_13990) proC 3107776..3108603 (+) 828 WP_039959707.1 pyrroline-5-carboxylate reductase -
  L0Y14_RS14015 (L0Y14_13995) - 3108600..3109178 (+) 579 WP_005959897.1 YggT family protein -
  L0Y14_RS14020 (L0Y14_14000) - 3109178..3109468 (+) 291 WP_005959896.1 DUF167 family protein -

Sequence

Protein


Download         Length: 375 a.a.        Molecular weight: 41968.29 Da        Isoelectric Point: 7.0164

>NTDB_id=643541 L0Y14_RS13995 WP_006474740.1 3104546..3105673(-) (pilU) [Candidatus Endoriftia persephone isolate Tica-EPR-9o50.N]
MDLNILLTMMVQKKASDLFITAGRAPSIKVDGRILQVSKAPLTMQQSRQYVMSVMSDVQQKEFESTRECNFAINAKGIGR
FRVSAFVQRDAVGMVMRRIETRIPTMEELRLPTVLQDLALSKRGLVIFVGATGTGKSTSLAAMIGFRNRKTSGHIISIED
PVEFIHSHEGCIITQREVGIDTDSYDVALKNTLRQAPDVILIGEIRSRETMQHALTFAETGHLCLSTLHANNANQALDRI
IHFFPEDQREQVFMDLSLNLRAVVAQQLIRRADGKGRRACIEVLLNSPYASELIRKGEIPKLKELMKRSTEQGMMTFDQN
LVALYQQGEITHEDALAYADSENDVRLAIRMAQTRTSDPILGGDLDDAMALSEHH

Nucleotide


Download         Length: 1128 bp        

>NTDB_id=643541 L0Y14_RS13995 WP_006474740.1 3104546..3105673(-) (pilU) [Candidatus Endoriftia persephone isolate Tica-EPR-9o50.N]
GTGGATCTTAATATATTGTTGACCATGATGGTTCAGAAGAAGGCGTCGGACCTCTTCATCACCGCGGGTCGCGCGCCCTC
GATCAAGGTGGATGGGCGCATTCTGCAAGTGAGCAAGGCACCACTGACGATGCAGCAGTCGCGCCAGTACGTGATGAGCG
TCATGAGCGATGTCCAGCAGAAAGAGTTTGAGTCGACCCGGGAGTGCAACTTTGCGATCAACGCCAAGGGCATTGGTCGC
TTCCGCGTCAGTGCCTTTGTGCAGCGGGATGCGGTGGGTATGGTGATGCGTCGCATCGAGACCCGCATCCCGACCATGGA
AGAGCTCAGGCTGCCCACTGTGCTGCAGGATCTAGCTCTCTCCAAACGTGGTCTGGTGATCTTTGTCGGTGCCACTGGTA
CCGGTAAATCGACCTCACTGGCGGCGATGATCGGCTTTCGCAACCGCAAGACCTCGGGCCACATCATCAGCATCGAAGAT
CCGGTGGAATTTATCCACAGCCATGAGGGCTGCATCATCACCCAGCGTGAGGTCGGTATCGATACCGACTCCTACGATGT
GGCGCTGAAGAACACCTTGCGCCAGGCGCCGGATGTGATCCTGATCGGTGAGATTCGTAGTCGCGAGACTATGCAGCATG
CGTTGACCTTTGCTGAGACCGGCCATCTCTGTCTCAGTACCCTGCACGCCAATAACGCCAATCAGGCTCTCGACCGGATC
ATCCACTTCTTTCCTGAAGATCAGCGTGAGCAGGTCTTCATGGACCTCTCACTCAACTTGCGCGCTGTTGTTGCGCAGCA
GTTGATTCGGCGTGCCGATGGTAAGGGACGGCGTGCCTGTATAGAGGTGTTGCTCAACTCCCCCTATGCCTCGGAGCTGA
TCCGTAAGGGCGAGATTCCCAAGCTGAAAGAGCTAATGAAGCGTTCCACCGAGCAGGGCATGATGACCTTCGATCAGAAT
CTGGTTGCTCTCTATCAGCAGGGTGAGATCACGCATGAAGATGCGCTGGCTTACGCTGATTCGGAGAACGATGTGCGTCT
GGCAATCAGAATGGCGCAGACCCGCACCAGTGATCCAATTCTTGGCGGCGACTTGGATGACGCCATGGCCCTGAGCGAGC
ATCACTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G2FFI0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

69.602

93.867

0.653
  pilU Acinetobacter baylyi ADP1

62.712

94.4

0.592
  pilU Vibrio cholerae strain A1552

57.766

97.867

0.565
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.413

93.067

0.413
  pilT Legionella pneumophila strain ERS1305867

40.588

90.667

0.368
  pilT Legionella pneumophila strain Lp02

40.588

90.667

0.368
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.768

87.467

0.365
  pilT Vibrio cholerae strain A1552

41.768

87.467

0.365