Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LY387_RS14360 Genome accession   NZ_CP090438
Coordinates   3157926..3158966 (+) Length   346 a.a.
NCBI ID   WP_234494593.1    Uniprot ID   -
Organism   Vibrio maritimus strain BH16     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3152926..3163966
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LY387_RS14325 hemW 3153210..3154379 (-) 1170 WP_234494587.1 radical SAM family heme chaperone HemW -
  LY387_RS14330 - 3154383..3154985 (-) 603 WP_234494588.1 XTP/dITP diphosphatase -
  LY387_RS14335 - 3155001..3155432 (-) 432 WP_234494589.1 DUF4426 domain-containing protein -
  LY387_RS14340 yggU 3155457..3155744 (-) 288 WP_042475818.1 DUF167 family protein YggU -
  LY387_RS14345 - 3155744..3156301 (-) 558 WP_128650533.1 YggT family protein -
  LY387_RS14350 proC 3156333..3157151 (-) 819 WP_234494591.1 pyrroline-5-carboxylate reductase -
  LY387_RS14355 - 3157190..3157900 (-) 711 WP_234494592.1 YggS family pyridoxal phosphate-dependent enzyme -
  LY387_RS14360 pilT 3157926..3158966 (+) 1041 WP_234494593.1 type IV pilus twitching motility protein PilT Machinery gene
  LY387_RS14365 pilU 3158976..3160085 (+) 1110 WP_234494594.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LY387_RS14395 - 3160883..3162505 (-) 1623 WP_234494595.1 methyl-accepting chemotaxis protein -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38588.10 Da        Isoelectric Point: 6.1088

>NTDB_id=642453 LY387_RS14360 WP_234494593.1 3157926..3158966(+) (pilT) [Vibrio maritimus strain BH16]
MDITELLDFSVKHNASDLHLSAGVSPMVRIDGEVRKLEIPSLEHSDVHRLIFDIMNDNQRSEYEEHLEIDFSFELPNIGR
FRVNAFNQSRGCAAVFRNIPTSPPTLEEIDAPRKFAELANLENGLVLVTGSTGSGKSTTLAAMVNHINEFQNKHILTIED
PIEFVHSNKKCLVNQREVHRDTHSFKAALRSALREDPDIILVGELRDHETISLALTAAETGHLVFGTMHTNSAAKSIDRI
IDVFPAQEKGMVRSMLSESLRAVIAQTLLKRLGGGRVACHEIMMGTPAIRNLIREDKVAQMFSVIQTGAAHGMQTMEQNA
QQLLSQGLVDVEEINKRVFIQQPMPM

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=642453 LY387_RS14360 WP_234494593.1 3157926..3158966(+) (pilT) [Vibrio maritimus strain BH16]
ATGGATATCACCGAGTTACTAGACTTCAGTGTAAAGCATAATGCATCAGATCTACACCTTTCTGCTGGGGTTTCCCCCAT
GGTTAGAATTGATGGTGAAGTCAGAAAACTGGAGATACCATCACTCGAACATTCTGATGTGCACCGGCTCATCTTTGACA
TCATGAATGACAACCAGCGCAGTGAATATGAAGAACATCTAGAAATCGACTTCTCCTTTGAACTCCCGAACATTGGTCGC
TTTCGCGTTAATGCATTTAATCAATCCCGCGGTTGTGCTGCCGTGTTTCGAAATATACCCACATCTCCTCCAACACTCGA
AGAGATTGACGCCCCAAGAAAGTTTGCAGAATTAGCGAACCTCGAAAATGGATTAGTGCTTGTGACAGGTTCTACAGGCT
CAGGAAAATCCACCACGCTTGCAGCAATGGTAAATCACATCAATGAGTTTCAAAACAAGCACATCCTTACTATTGAAGAT
CCCATTGAGTTTGTGCATAGCAATAAGAAATGCCTAGTCAATCAACGAGAAGTACACCGAGATACCCATAGCTTTAAAGC
AGCCCTCAGATCGGCGCTTAGAGAAGATCCTGACATCATCCTAGTGGGTGAATTAAGAGACCATGAAACCATCAGTCTCG
CACTGACCGCGGCCGAGACTGGGCACTTAGTGTTCGGCACAATGCATACGAATTCCGCTGCAAAATCCATTGACCGAATC
ATTGACGTTTTCCCGGCACAGGAGAAAGGTATGGTCAGATCTATGCTGTCTGAATCATTAAGAGCGGTGATTGCCCAAAC
ACTGCTAAAACGCCTCGGTGGTGGCCGGGTAGCATGTCACGAGATTATGATGGGAACCCCTGCGATCAGGAATCTAATAC
GTGAAGACAAGGTAGCTCAAATGTTTTCGGTCATTCAAACAGGAGCTGCGCACGGCATGCAAACGATGGAGCAGAATGCT
CAGCAACTCTTATCACAAGGGTTGGTCGATGTTGAGGAGATAAATAAAAGAGTCTTCATACAACAACCGATGCCTATGTA
G

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

81.065

97.688

0.792
  pilT Vibrio cholerae strain A1552

81.065

97.688

0.792
  pilT Acinetobacter nosocomialis M2

69.096

99.133

0.685
  pilT Acinetobacter baumannii D1279779

68.805

99.133

0.682
  pilT Acinetobacter baumannii strain A118

68.805

99.133

0.682
  pilT Acinetobacter baylyi ADP1

70.393

95.665

0.673
  pilT Pseudomonas aeruginosa PAK

67.251

98.844

0.665
  pilT Pseudomonas stutzeri DSM 10701

66.764

99.133

0.662
  pilT Legionella pneumophila strain Lp02

68.997

95.087

0.656
  pilT Legionella pneumophila strain ERS1305867

68.997

95.087

0.656
  pilT Neisseria meningitidis 8013

63.609

97.688

0.621
  pilT Neisseria gonorrhoeae MS11

63.314

97.688

0.619
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.599

96.532

0.488
  pilU Vibrio cholerae strain A1552

41.846

93.931

0.393
  pilU Pseudomonas stutzeri DSM 10701

40.12

96.532

0.387
  pilU Acinetobacter baylyi ADP1

40

92.486

0.37