Detailed information NTG0642454

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: LY387_RS14365
• Genome accession: NZ_CP090438
• Coordinates: 3158976..3160085 (+)
• Length: 369 a.a.
• NCBI ID: WP_234494594.1
• Uniprot ID: -
• Organism: Vibrio maritimus strain BH16
• Function: type IV pilus retraction (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3153976..3165085

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LY387_RS14330	-	3154383..3154985 (-)	603	WP_234494588.1	XTP/dITP diphosphatase	-
LY387_RS14335	-	3155001..3155432 (-)	432	WP_234494589.1	DUF4426 domain-containing protein	-
LY387_RS14340	yggU	3155457..3155744 (-)	288	WP_042475818.1	DUF167 family protein YggU	-
LY387_RS14345	-	3155744..3156301 (-)	558	WP_128650533.1	YggT family protein	-
LY387_RS14350	proC	3156333..3157151 (-)	819	WP_234494591.1	pyrroline-5-carboxylate reductase	-
LY387_RS14355	-	3157190..3157900 (-)	711	WP_234494592.1	YggS family pyridoxal phosphate-dependent enzyme	-
LY387_RS14360	pilT	3157926..3158966 (+)	1041	WP_234494593.1	type IV pilus twitching motility protein PilT	Machinery gene
LY387_RS14365	pilU	3158976..3160085 (+)	1110	WP_234494594.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
LY387_RS14395	-	3160883..3162505 (-)	1623	WP_234494595.1	methyl-accepting chemotaxis protein	-

Sequence

Protein

• Length: 369 a.a.
• Molecular weight: 41406.30 Da
• Isoelectric Point: 7.0761

>NTDB_id=642454 LY387_RS14365 WP_234494594.1 3158976..3160085(+) (pilU) [Vibrio maritimus strain BH16]
MDTMDNILKQMLACRASDLYLTADAPVLFRVDGDLKPHGSALHHHDVHSLLQEVMDSSSYQQYQASKEANFAIVKTYGRF
RVSAFFQRELPGAVIRRIETHIPSFEELALPDVVQDLAIAKRGLVLIVGGTGSGKSTTMAAMTGYRNRHRSGHILTVEDP
IEFVHQHQKCIVTQREVGLDTESYDIALKNSLRQAPDMIVIGEIRNRSTMEYAMAFAETGHLCMATLHANNANQALERIL
HLVPKERKEQFLFDLSANLRGIVAQQLIRDKYAQGRHGVFEVMLSSPRISDLIRKGALHELKAAMTKSNDVGMQTFDQAL
YQLVRAGKINQEDALHSADSQNDLRLLLKFQESESQESNPLRGIKIDMS

Nucleotide

• Length: 1110 bp

>NTDB_id=642454 LY387_RS14365 WP_234494594.1 3158976..3160085(+) (pilU) [Vibrio maritimus strain BH16]
ATGGATACGATGGATAATATTTTGAAGCAGATGCTCGCGTGTAGAGCGTCCGATCTTTATTTAACCGCGGATGCCCCCGT
CTTATTCAGAGTGGATGGAGATCTAAAGCCGCACGGCTCAGCCCTGCATCACCACGATGTTCACTCACTGCTACAAGAGG
TAATGGATAGCTCGAGTTATCAACAATACCAAGCAAGTAAAGAAGCTAATTTTGCCATTGTTAAGACTTATGGGCGCTTC
CGTGTGAGCGCGTTCTTTCAAAGAGAACTGCCAGGTGCGGTCATAAGGCGTATCGAAACTCACATTCCCAGTTTTGAAGA
ACTCGCACTTCCTGATGTAGTACAAGATCTTGCTATCGCTAAGCGTGGACTAGTGCTGATCGTTGGAGGAACTGGCTCTG
GTAAGTCTACAACCATGGCGGCAATGACAGGTTACAGGAACCGCCACCGCAGTGGACATATACTGACGGTGGAGGACCCT
ATCGAGTTCGTCCACCAACATCAAAAATGCATTGTTACGCAGAGGGAAGTAGGGCTAGACACTGAAAGCTACGACATCGC
ACTCAAAAACTCTCTTCGACAAGCACCAGATATGATCGTCATTGGCGAGATTCGTAACCGTTCAACCATGGAGTACGCAA
TGGCTTTTGCCGAAACGGGTCACCTATGTATGGCAACACTACATGCGAACAACGCTAATCAAGCCTTAGAGAGGATACTT
CACTTAGTGCCAAAAGAGCGTAAGGAGCAGTTCCTGTTTGATCTCTCAGCGAACCTCAGAGGCATTGTCGCTCAGCAGCT
TATTAGAGACAAATATGCACAAGGTAGGCATGGGGTTTTTGAGGTTATGCTGAGCAGTCCAAGAATCTCAGACCTTATCC
GCAAAGGCGCGCTGCACGAGCTCAAAGCAGCAATGACGAAAAGTAATGATGTTGGAATGCAAACATTTGATCAGGCGCTA
TATCAATTGGTTAGGGCAGGAAAGATCAATCAAGAAGACGCTTTACACAGTGCAGACTCTCAAAATGATCTCAGGCTATT
GCTAAAGTTTCAAGAAAGTGAATCTCAAGAGAGCAATCCGCTTAGGGGGATTAAGATTGATATGAGTTAG

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (7-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Vibrio cholerae strain A1552	71.781	98.916	0.71
pilU	Pseudomonas stutzeri DSM 10701	53.514	100	0.537
pilU	Acinetobacter baylyi ADP1	48.596	96.477	0.469
pilT	Legionella pneumophila strain ERS1305867	42.331	88.347	0.374
pilT	Legionella pneumophila strain Lp02	42.331	88.347	0.374
pilT	Acinetobacter baylyi ADP1	41.768	88.889	0.371
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	40.299	90.786	0.366
pilT	Acinetobacter nosocomialis M2	41.231	88.076	0.363
pilT	Acinetobacter baumannii D1279779	41.231	88.076	0.363
pilT	Acinetobacter baumannii strain A118	41.231	88.076	0.363
pilT	Pseudomonas aeruginosa PAK	40.923	88.076	0.36