Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LY624_RS03475 Genome accession   NZ_CP090419
Coordinates   727538..728578 (+) Length   346 a.a.
NCBI ID   WP_062570117.1    Uniprot ID   A0AAQ1CBU3
Organism   Pseudoalteromonas sp. N1230-9     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 722538..733578
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LY624_RS03445 (LY624_03440) hemW 723190..724326 (-) 1137 WP_341803874.1 radical SAM family heme chaperone HemW -
  LY624_RS03450 (LY624_03445) - 724323..724922 (-) 600 WP_130150758.1 XTP/dITP diphosphatase -
  LY624_RS03455 (LY624_03450) - 724930..725370 (-) 441 WP_341803875.1 DUF4426 domain-containing protein -
  LY624_RS03460 (LY624_03455) - 725425..725958 (-) 534 WP_062570114.1 YggT family protein -
  LY624_RS03465 (LY624_03460) proC 725983..726804 (-) 822 WP_341803876.1 pyrroline-5-carboxylate reductase -
  LY624_RS03470 (LY624_03465) - 726822..727535 (-) 714 WP_341803877.1 YggS family pyridoxal phosphate-dependent enzyme -
  LY624_RS03475 (LY624_03470) pilT 727538..728578 (+) 1041 WP_062570117.1 type IV pilus twitching motility protein PilT Machinery gene
  LY624_RS03480 (LY624_03475) pilU 728593..729726 (+) 1134 WP_062570118.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LY624_RS03485 (LY624_03480) - 729732..730595 (+) 864 WP_341803878.1 hypothetical protein -
  LY624_RS03490 (LY624_03485) ruvX 730592..731041 (-) 450 WP_237120139.1 Holliday junction resolvase RuvX -
  LY624_RS03495 (LY624_03490) - 731072..731629 (-) 558 WP_341803879.1 YqgE/AlgH family protein -
  LY624_RS03500 (LY624_03495) gshB 731654..732607 (-) 954 WP_130150763.1 glutathione synthase -
  LY624_RS03505 (LY624_03500) rsmE 732676..733410 (-) 735 WP_062570123.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38179.42 Da        Isoelectric Point: 6.0831

>NTDB_id=642214 LY624_RS03475 WP_062570117.1 727538..728578(+) (pilT) [Pseudoalteromonas sp. N1230-9]
MDITELLAFSVQHKASDLHLSSGVSPMIRVDGDVRRINIPALEQKDVNSLVYDIMNDNQRKDYEQNLEVDFSFEVPNLAR
FRVNAFNSNRGPAAVFRTIPSDVLTLDDLGAPEIFKTIADNPRGLVLVTGPTGSGKSTTLAAMVDYINQTKHHHILTIED
PIEFVHDNKLSLINQREVHRDTHSFSNALRSALREDPDVILVGELRDLETIRLAMTAAETGHLVFGTLHTTSAPKTIDRI
IDVFPGEEKDMVRSMLSESLRAVISQTLLKKVGGGRVAAHEIMIGIPAIRNLIREDKIAQMYSSIQTGASHGMQTMDQCL
GNLVNYGIVTTADARAKAQDKTQFGG

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=642214 LY624_RS03475 WP_062570117.1 727538..728578(+) (pilT) [Pseudoalteromonas sp. N1230-9]
ATGGATATTACCGAATTATTGGCCTTTAGTGTGCAACATAAGGCCTCGGATTTACACTTATCATCGGGTGTATCACCTAT
GATACGCGTCGATGGCGATGTACGTCGTATTAATATTCCAGCATTAGAACAAAAAGATGTAAACAGTCTGGTTTACGATA
TTATGAACGATAATCAGCGTAAGGACTATGAGCAAAATCTCGAAGTGGATTTTTCTTTTGAAGTGCCTAATCTTGCTCGT
TTTCGTGTTAACGCGTTTAATTCAAACCGAGGACCCGCAGCTGTATTTCGTACAATCCCCAGTGATGTGTTAACACTTGA
TGATTTAGGTGCACCGGAAATATTTAAGACTATTGCTGATAACCCACGCGGTTTGGTTTTAGTCACTGGGCCAACAGGTT
CAGGTAAATCAACCACCTTAGCAGCGATGGTTGATTACATTAATCAGACCAAGCACCATCATATTTTAACGATTGAAGAT
CCAATCGAATTTGTTCACGACAATAAGCTGAGCCTTATTAACCAACGCGAAGTTCATCGTGATACTCACAGCTTCTCTAA
TGCTTTACGTAGTGCGTTACGTGAAGATCCTGATGTAATTCTGGTGGGTGAGCTACGAGATTTAGAAACGATTCGCTTAG
CCATGACGGCCGCAGAAACAGGTCATTTAGTGTTCGGCACTTTGCATACCACATCTGCACCAAAGACTATTGACCGTATT
ATCGATGTATTCCCAGGTGAAGAAAAAGACATGGTGCGCTCAATGCTTTCAGAATCGTTGCGTGCCGTTATTTCGCAAAC
ATTACTTAAAAAAGTAGGGGGTGGGCGAGTGGCAGCGCACGAGATTATGATAGGGATCCCTGCTATTCGAAATCTTATTC
GTGAGGATAAAATTGCACAGATGTACTCATCGATTCAAACTGGCGCATCACATGGTATGCAAACGATGGATCAATGTTTA
GGTAATTTAGTGAACTACGGCATTGTTACTACAGCAGATGCTAGAGCTAAAGCGCAAGATAAAACTCAGTTTGGTGGCTA
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

77.616

99.422

0.772
  pilT Acinetobacter baumannii strain A118

77.616

99.422

0.772
  pilT Acinetobacter nosocomialis M2

77.616

99.422

0.772
  pilT Acinetobacter baylyi ADP1

77.035

99.422

0.766
  pilT Pseudomonas stutzeri DSM 10701

77.035

99.422

0.766
  pilT Pseudomonas aeruginosa PAK

76.163

99.422

0.757
  pilT Legionella pneumophila strain Lp02

72.384

99.422

0.72
  pilT Legionella pneumophila strain ERS1305867

72.384

99.422

0.72
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.976

97.977

0.705
  pilT Vibrio cholerae strain A1552

71.976

97.977

0.705
  pilT Neisseria meningitidis 8013

70.968

98.555

0.699
  pilT Neisseria gonorrhoeae MS11

70.674

98.555

0.697
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.174

99.711

0.52
  pilU Vibrio cholerae strain A1552

42.09

96.821

0.408
  pilU Acinetobacter baylyi ADP1

39.412

98.266

0.387
  pilU Pseudomonas stutzeri DSM 10701

39.701

96.821

0.384