Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   LY624_RS03480 Genome accession   NZ_CP090419
Coordinates   728593..729726 (+) Length   377 a.a.
NCBI ID   WP_062570118.1    Uniprot ID   A0AAQ1CBU1
Organism   Pseudoalteromonas sp. N1230-9     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 723593..734726
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LY624_RS03450 (LY624_03445) - 724323..724922 (-) 600 WP_130150758.1 XTP/dITP diphosphatase -
  LY624_RS03455 (LY624_03450) - 724930..725370 (-) 441 WP_341803875.1 DUF4426 domain-containing protein -
  LY624_RS03460 (LY624_03455) - 725425..725958 (-) 534 WP_062570114.1 YggT family protein -
  LY624_RS03465 (LY624_03460) proC 725983..726804 (-) 822 WP_341803876.1 pyrroline-5-carboxylate reductase -
  LY624_RS03470 (LY624_03465) - 726822..727535 (-) 714 WP_341803877.1 YggS family pyridoxal phosphate-dependent enzyme -
  LY624_RS03475 (LY624_03470) pilT 727538..728578 (+) 1041 WP_062570117.1 type IV pilus twitching motility protein PilT Machinery gene
  LY624_RS03480 (LY624_03475) pilU 728593..729726 (+) 1134 WP_062570118.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LY624_RS03485 (LY624_03480) - 729732..730595 (+) 864 WP_341803878.1 hypothetical protein -
  LY624_RS03490 (LY624_03485) ruvX 730592..731041 (-) 450 WP_237120139.1 Holliday junction resolvase RuvX -
  LY624_RS03495 (LY624_03490) - 731072..731629 (-) 558 WP_341803879.1 YqgE/AlgH family protein -
  LY624_RS03500 (LY624_03495) gshB 731654..732607 (-) 954 WP_130150763.1 glutathione synthase -
  LY624_RS03505 (LY624_03500) rsmE 732676..733410 (-) 735 WP_062570123.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  LY624_RS03510 (LY624_03505) - 733470..733934 (-) 465 WP_130150764.1 hypothetical protein -

Sequence

Protein


Download         Length: 377 a.a.        Molecular weight: 41784.75 Da        Isoelectric Point: 5.9727

>NTDB_id=642215 LY624_RS03480 WP_062570118.1 728593..729726(+) (pilU) [Pseudoalteromonas sp. N1230-9]
MTIPLNRFLQVMIDKNGSDLFVSSGLPVSAKINGELQPLTDDKLSDEQALAMVESAMNEKQRNEFHATKECNFAIATEEG
RFRVSAFWQRDQAGMVIRRIVTQIPDVNDLGLPSILTDVIMAKRGLVLFVGGTGTGKSTSLAALLGYRNRNQRGHILTIE
DPIEFVHEHQKSIITQREVGLDTESFESALKSSLRQAPDVILIGEIRSQETMEYALSFAETGHLCVATLHANNANQAIDR
IMHLVPKEKHDKLKYDLALNLRAIVAQQLVPTADGEGRVAAIEILLNSPMVAELIKRGDIGSIKETMAKSKEMGMQTFDQ
ALFELYKHQRINYADALHHADSPNDLRLMIKLQNNEQKGAGFLQGVTIDGLDEKGNS

Nucleotide


Download         Length: 1134 bp        

>NTDB_id=642215 LY624_RS03480 WP_062570118.1 728593..729726(+) (pilU) [Pseudoalteromonas sp. N1230-9]
ATGACAATACCTTTAAATCGCTTTTTACAGGTGATGATCGACAAGAATGGTTCCGATTTATTTGTGTCCAGTGGCTTACC
TGTTAGTGCCAAAATTAACGGTGAACTACAGCCGCTGACCGACGATAAGTTATCTGATGAGCAGGCACTGGCTATGGTTG
AGTCTGCGATGAACGAAAAGCAACGTAATGAGTTTCACGCAACAAAAGAATGTAACTTTGCTATTGCAACAGAAGAAGGC
CGGTTTCGTGTTTCTGCATTTTGGCAGCGAGACCAAGCGGGTATGGTTATTCGCCGAATTGTGACGCAAATTCCTGATGT
AAACGATTTAGGATTACCGTCTATTCTTACCGATGTGATAATGGCAAAACGAGGCTTGGTGCTGTTTGTAGGGGGGACCG
GTACCGGTAAGTCAACCTCGCTTGCTGCGCTTTTAGGTTATCGAAATCGTAATCAACGTGGGCACATTCTTACTATTGAA
GATCCTATCGAGTTTGTGCATGAACACCAAAAAAGTATTATTACTCAACGCGAAGTAGGTCTTGATACTGAAAGCTTTGA
GTCAGCGTTGAAAAGCTCATTACGCCAAGCACCTGATGTTATTTTAATTGGTGAAATTCGTTCACAAGAAACCATGGAAT
ATGCCTTAAGCTTTGCTGAAACAGGGCATTTATGTGTCGCCACCCTGCATGCTAATAATGCTAACCAAGCCATTGACCGT
ATTATGCACTTAGTGCCAAAAGAAAAACACGATAAGTTAAAATACGACTTAGCACTGAACCTACGCGCAATTGTTGCACA
GCAACTGGTGCCAACAGCGGATGGGGAAGGCCGTGTGGCTGCTATCGAGATCTTGCTTAATTCTCCTATGGTCGCAGAGC
TTATAAAACGCGGTGACATTGGTTCAATTAAAGAAACTATGGCGAAATCAAAAGAAATGGGTATGCAGACTTTTGACCAA
GCCTTGTTTGAGCTTTATAAACACCAGCGTATTAATTATGCCGATGCCTTGCATCATGCCGACTCGCCAAACGATTTACG
TTTGATGATCAAGTTACAAAATAACGAGCAAAAAGGGGCTGGCTTTTTACAAGGTGTGACTATTGACGGCCTTGATGAAA
AGGGTAATAGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(16-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

57.838

98.143

0.568
  pilU Acinetobacter baylyi ADP1

56.533

99.469

0.562
  pilU Vibrio cholerae strain A1552

56.403

97.347

0.549
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.056

95.491

0.411
  pilT Acinetobacter baumannii strain A118

41.088

87.798

0.361
  pilT Acinetobacter nosocomialis M2

41.088

87.798

0.361
  pilT Acinetobacter baumannii D1279779

41.088

87.798

0.361
  pilT Acinetobacter baylyi ADP1

40

90.186

0.361