Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LZG54_RS03475 Genome accession   NZ_CP090382
Coordinates   721363..722400 (-) Length   345 a.a.
NCBI ID   WP_180064767.1    Uniprot ID   -
Organism   Acinetobacter towneri strain SCLZS30     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 716363..727400
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LZG54_RS03445 (LZG54_03460) - 717101..717556 (+) 456 WP_234114605.1 bacteriohemerythrin -
  LZG54_RS03450 (LZG54_03465) - 717746..718831 (+) 1086 WP_234114606.1 hypothetical protein -
  LZG54_RS03455 (LZG54_03470) - 718828..719148 (+) 321 WP_096902265.1 RnfH family protein -
  LZG54_RS03460 (LZG54_03475) - 719204..719602 (-) 399 WP_178318246.1 outer membrane protein assembly factor BamE -
  LZG54_RS03465 (LZG54_03480) fur 719721..720158 (+) 438 WP_004975325.1 ferric iron uptake transcriptional regulator -
  LZG54_RS03470 (LZG54_03485) pilU 720223..721341 (-) 1119 WP_234114607.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LZG54_RS03475 (LZG54_03490) pilT 721363..722400 (-) 1038 WP_180064767.1 type IV pilus twitching motility protein PilT Machinery gene
  LZG54_RS03480 (LZG54_03495) - 722525..723214 (+) 690 WP_234114608.1 YggS family pyridoxal phosphate-dependent enzyme -
  LZG54_RS03485 (LZG54_03500) - 723571..723846 (-) 276 WP_004975329.1 hypothetical protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38227.74 Da        Isoelectric Point: 6.4194

>NTDB_id=641876 LZG54_RS03475 WP_180064767.1 721363..722400(-) (pilT) [Acinetobacter towneri strain SCLZS30]
MDITELLAFSAKNGASDLHLSAGMPPLIRVDGEVRRINLPALEHKEVHKLVYDIMNDKQRRDYEENLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLTLEDLGMGQIFKDICEYPRGLVLVTGPTGSGKSTTLAAMIDFINDNRYDHILTVED
PIEFVHQSKKCLVNQREVNRDTHGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAEEKDMVRAMLSESLQAVISQSLLKKNGGGRVAAHEIMIGIPAVRNLIRENKVAQMYSAIQTGANYGMTTLDQSL
KNLVSRGVISPQTARTVAKQPESFL

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=641876 LZG54_RS03475 WP_180064767.1 721363..722400(-) (pilT) [Acinetobacter towneri strain SCLZS30]
ATGGATATTACAGAATTATTGGCGTTCTCCGCTAAAAACGGCGCTTCGGATTTACACTTATCTGCGGGCATGCCACCACT
GATTCGTGTGGATGGTGAAGTACGTCGAATTAACTTACCAGCATTAGAACATAAAGAAGTACACAAATTAGTGTACGACA
TTATGAATGATAAACAACGTCGTGATTATGAAGAAAATCTAGAAACGGACTTTTCTTTTGAAGTCCCAGGCGTGGCTCGT
TTCCGTGTGAACGCATTTAACCAAAACCGTGGTGCAGGTGCAGTATTCCGTACCATTCCTTCTAAAGTTTTGACCCTTGA
AGATTTAGGCATGGGACAAATCTTTAAAGATATTTGTGAATATCCACGCGGTTTGGTTTTGGTGACAGGCCCAACGGGTT
CAGGTAAATCGACCACACTTGCCGCGATGATTGACTTTATTAATGACAATCGCTACGACCATATTCTGACCGTCGAAGAC
CCAATCGAATTTGTACACCAATCTAAAAAATGTTTGGTTAACCAACGAGAAGTAAACCGTGATACGCATGGCTTTAACGA
AGCATTACGTTCGGCACTGCGTGAAGACCCTGACATTATTCTAGTGGGTGAGATGCGTGACCTAGAAACCATTCGTTTGG
CACTGACGGCTGCCGAAACAGGTCACTTGGTGTTTGGTACGCTACACACCACCTCTGCAGCAAAAACCATTGACCGTGTG
ATTGACGTATTCCCTGCCGAAGAAAAAGACATGGTACGCGCCATGTTATCTGAATCTTTACAAGCCGTCATTTCACAATC
CCTGCTCAAGAAAAATGGCGGTGGTCGTGTAGCAGCGCATGAAATCATGATTGGTATTCCTGCGGTGAGAAACTTGATTC
GTGAAAATAAAGTAGCTCAAATGTACTCGGCAATTCAGACAGGTGCCAATTACGGTATGACTACCCTCGACCAGAGTTTG
AAGAACTTGGTATCGCGCGGTGTGATTAGCCCACAAACGGCTCGAACGGTTGCAAAACAACCTGAATCATTCTTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

94.203

100

0.942
  pilT Acinetobacter baumannii D1279779

93.913

100

0.939
  pilT Acinetobacter baumannii strain A118

93.913

100

0.939
  pilT Acinetobacter baylyi ADP1

86.047

99.71

0.858
  pilT Pseudomonas aeruginosa PAK

82.849

99.71

0.826
  pilT Pseudomonas stutzeri DSM 10701

82.558

99.71

0.823
  pilT Legionella pneumophila strain Lp02

74.128

99.71

0.739
  pilT Legionella pneumophila strain ERS1305867

74.128

99.71

0.739
  pilT Neisseria meningitidis 8013

68.406

100

0.684
  pilT Neisseria gonorrhoeae MS11

68.116

100

0.681
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.75

97.391

0.67
  pilT Vibrio cholerae strain A1552

68.75

97.391

0.67
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.174

100

0.522
  pilU Pseudomonas stutzeri DSM 10701

41.493

97.101

0.403
  pilU Vibrio cholerae strain A1552

41.493

97.101

0.403
  pilU Acinetobacter baylyi ADP1

38.439

100

0.386