Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   LZG54_RS03470 Genome accession   NZ_CP090382
Coordinates   720223..721341 (-) Length   372 a.a.
NCBI ID   WP_234114607.1    Uniprot ID   -
Organism   Acinetobacter towneri strain SCLZS30     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 715223..726341
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LZG54_RS03440 (LZG54_03455) - 715990..716838 (+) 849 WP_234114604.1 class II glutamine amidotransferase -
  LZG54_RS03445 (LZG54_03460) - 717101..717556 (+) 456 WP_234114605.1 bacteriohemerythrin -
  LZG54_RS03450 (LZG54_03465) - 717746..718831 (+) 1086 WP_234114606.1 hypothetical protein -
  LZG54_RS03455 (LZG54_03470) - 718828..719148 (+) 321 WP_096902265.1 RnfH family protein -
  LZG54_RS03460 (LZG54_03475) - 719204..719602 (-) 399 WP_178318246.1 outer membrane protein assembly factor BamE -
  LZG54_RS03465 (LZG54_03480) fur 719721..720158 (+) 438 WP_004975325.1 ferric iron uptake transcriptional regulator -
  LZG54_RS03470 (LZG54_03485) pilU 720223..721341 (-) 1119 WP_234114607.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LZG54_RS03475 (LZG54_03490) pilT 721363..722400 (-) 1038 WP_180064767.1 type IV pilus twitching motility protein PilT Machinery gene
  LZG54_RS03480 (LZG54_03495) - 722525..723214 (+) 690 WP_234114608.1 YggS family pyridoxal phosphate-dependent enzyme -
  LZG54_RS03485 (LZG54_03500) - 723571..723846 (-) 276 WP_004975329.1 hypothetical protein -

Sequence

Protein


Download         Length: 372 a.a.        Molecular weight: 41798.07 Da        Isoelectric Point: 6.8894

>NTDB_id=641875 LZG54_RS03470 WP_234114607.1 720223..721341(-) (pilU) [Acinetobacter towneri strain SCLZS30]
MDFNDLLNLMIQQQASDLFITANVEPSMKIHGQIVPVAKSKLTGEIIGQLLNSIMSEKQRKEFAETRECNFAITNREKTA
RFRVSAFQQRDEPGMVLRRIETKIPAMDDLKLPPILKDLAMTKRGIIIFVGATGTGKSTSLASIIGYRNQNSKGHIITIE
DPIEFVHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSRETMNYAIAFAETGHLVFATLHANNANQAIDR
IIHFFEADRHNQLFMDLSLNLKAIVAQQLIPTIDGNSRRAAIEILINSPLVADLIRKGDVHEIKDLMKRSRELGMQTFDQ
ALYDLYKAKQISYKDALKHADSPNDLRLQIKLAEEGGERLLHATSNITFDGQ

Nucleotide


Download         Length: 1119 bp        

>NTDB_id=641875 LZG54_RS03470 WP_234114607.1 720223..721341(-) (pilU) [Acinetobacter towneri strain SCLZS30]
ATGGACTTTAATGATTTACTGAATTTAATGATTCAGCAACAAGCATCAGACTTGTTTATTACAGCCAATGTTGAACCTTC
CATGAAAATTCATGGACAAATTGTACCTGTGGCAAAAAGTAAGCTCACAGGTGAAATTATCGGTCAGCTGCTTAACTCAA
TTATGAGTGAAAAACAGCGCAAGGAATTTGCCGAAACCCGTGAATGTAACTTTGCGATCACAAACCGTGAAAAAACAGCA
CGTTTTCGTGTCAGCGCATTCCAGCAACGTGATGAGCCGGGCATGGTACTCCGTCGGATTGAAACCAAAATTCCGGCCAT
GGATGACTTGAAACTGCCACCTATTCTCAAAGATTTGGCGATGACCAAACGTGGCATCATCATTTTTGTAGGTGCGACAG
GTACAGGTAAATCGACCTCTTTAGCGTCTATTATTGGTTACCGCAACCAAAATTCCAAAGGTCATATCATTACCATTGAA
GACCCGATTGAATTTGTGCATGAACATGCAGGCTGTATTATTACCCAGCGTGAAGTCGGGATTGATACCGACTCCTTTGA
AATCGCATTAAAAAATACCTTGCGTCAAGCGCCTGATGTAATTCTGATTGGTGAGATTCGTTCCCGCGAAACCATGAATT
ATGCAATTGCCTTTGCCGAAACAGGTCACTTGGTATTTGCCACCCTACACGCCAACAACGCTAACCAAGCAATTGACCGT
ATTATTCACTTCTTTGAAGCAGACCGTCATAACCAGTTGTTCATGGATTTATCGCTAAACCTGAAAGCAATTGTGGCACA
GCAGTTAATTCCAACCATTGATGGCAACTCACGTCGTGCTGCGATTGAAATTTTAATTAACTCACCATTAGTTGCAGATT
TAATCCGTAAAGGTGATGTGCACGAAATTAAAGACCTGATGAAGCGTTCACGTGAGTTGGGTATGCAAACCTTTGACCAA
GCGTTATACGATCTTTATAAAGCCAAACAAATTAGCTACAAAGATGCACTTAAACACGCCGACTCGCCAAACGATTTGCG
TCTACAAATCAAACTTGCAGAAGAAGGTGGCGAGCGCTTGTTACATGCAACTTCGAATATTACCTTCGATGGGCAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

75.926

100

0.772
  pilU Pseudomonas stutzeri DSM 10701

68.194

99.731

0.68
  pilU Vibrio cholerae strain A1552

55.271

94.355

0.522
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.486

93.011

0.395
  pilT Legionella pneumophila strain Lp02

41.124

90.86

0.374
  pilT Legionella pneumophila strain ERS1305867

41.124

90.86

0.374
  pilT Acinetobacter nosocomialis M2

40.233

92.204

0.371
  pilT Acinetobacter baumannii D1279779

40.233

92.204

0.371
  pilT Acinetobacter baumannii strain A118

40.233

92.204

0.371
  pilT Pseudomonas aeruginosa PAK

39.595

93.011

0.368
  pilT Vibrio cholerae strain A1552

38.177

94.355

0.36
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

38.177

94.355

0.36
  pilT Neisseria gonorrhoeae MS11

39.412

91.398

0.36
  pilT Neisseria meningitidis 8013

39.412

91.398

0.36
  pilT Acinetobacter baylyi ADP1

39.067

92.204

0.36
  pilT Pseudomonas stutzeri DSM 10701

38.728

93.011

0.36