Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LW136_RS01460 Genome accession   NZ_CP090366
Coordinates   301777..302811 (+) Length   344 a.a.
NCBI ID   WP_003303153.1    Uniprot ID   A0A1I3QZE2
Organism   Stutzerimonas kunmingensis strain 7850S     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 296777..307811
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LW136_RS01440 (LW136_01440) - 297309..299276 (-) 1968 WP_234174504.1 dynamin-like GTPase family protein -
  LW136_RS01445 (LW136_01445) - 299547..300137 (-) 591 WP_014818744.1 YggT family protein -
  LW136_RS01450 (LW136_01450) proC 300188..301012 (-) 825 WP_234174505.1 pyrroline-5-carboxylate reductase -
  LW136_RS01455 (LW136_01455) - 301023..301712 (-) 690 WP_234174506.1 YggS family pyridoxal phosphate-dependent enzyme -
  LW136_RS01460 (LW136_01460) pilT 301777..302811 (+) 1035 WP_003303153.1 type IV pilus twitching motility protein PilT Machinery gene
  LW136_RS01465 (LW136_01465) pilU 302872..304017 (+) 1146 WP_014818747.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LW136_RS01470 (LW136_01470) - 304014..304850 (-) 837 WP_234174507.1 TIGR03915 family putative DNA repair protein -
  LW136_RS01475 (LW136_01475) - 304850..306061 (-) 1212 WP_102832393.1 putative DNA modification/repair radical SAM protein -
  LW136_RS01480 (LW136_01480) - 306195..306590 (+) 396 WP_003282737.1 NINE protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37946.67 Da        Isoelectric Point: 6.8360

>NTDB_id=641713 LW136_RS01460 WP_003303153.1 301777..302811(+) (pilT) [Stutzerimonas kunmingensis strain 7850S]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPAMDHKQVHALIYDIMNDKQRKDFEEFLETDFSFEVPGVAR
FRVNAFNQNRGSGAVFRTIPSKVLTMEDLGMGEVFRKITDVPRGLVLVTGPTGSGKSTTLAAMLDYLNNTKYHHILTIED
PIEFVHESKKCLVNQREVHRDTLGFSEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAEEKSMVRSMLSESLQAVISQTLLKKVGGGRVAAHEIMIGTPAIRNLIREDKVAQMYSSIQTGGSLGMQTLDSCL
KGLLAKGLISRDSAKEKAKQPENF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=641713 LW136_RS01460 WP_003303153.1 301777..302811(+) (pilT) [Stutzerimonas kunmingensis strain 7850S]
ATGGATATCACAGAACTGCTGGCCTTCAGCGCCAAGCAGGGTGCGTCGGATTTGCACCTTTCCGCCGGCCTGCCGCCGAT
GATCCGCGTCGATGGCGACGTGCGGCGAATCAACCTGCCGGCCATGGACCACAAGCAGGTGCATGCGCTGATCTACGACA
TCATGAACGACAAGCAGCGCAAGGATTTCGAGGAATTCCTGGAAACCGACTTCTCGTTCGAAGTGCCGGGCGTGGCGCGT
TTTCGCGTCAACGCGTTCAACCAGAATCGCGGTTCCGGCGCGGTGTTCCGGACCATTCCTTCCAAGGTGCTGACCATGGA
AGACCTGGGGATGGGCGAGGTGTTCCGCAAGATCACCGATGTGCCGCGCGGGCTGGTGCTGGTCACCGGACCGACCGGCT
CGGGCAAGTCGACCACCCTGGCGGCGATGCTTGATTACCTGAACAACACCAAGTACCACCACATCCTTACCATCGAGGAC
CCCATCGAATTCGTTCACGAATCGAAGAAGTGTCTGGTCAACCAGCGCGAGGTCCATCGTGACACCCTGGGCTTCTCCGA
GGCACTGCGTTCGGCACTGCGTGAAGACCCGGACATCATTCTCGTCGGCGAAATGCGCGACCTGGAAACCATCCGCCTGG
CGCTGACCGCTGCGGAAACCGGTCACCTGGTGTTCGGCACCCTGCACACCACCTCCGCCGCCAAGACCATCGACCGCGTG
GTCGACGTGTTCCCGGCCGAAGAAAAGTCCATGGTCCGTTCGATGCTCTCCGAATCGCTGCAGGCGGTGATCTCGCAGAC
CCTGCTGAAGAAGGTTGGCGGCGGCCGTGTCGCGGCCCACGAGATCATGATCGGTACGCCGGCGATCCGTAACCTGATTC
GCGAGGACAAGGTGGCGCAGATGTATTCCTCGATCCAGACCGGCGGCTCGCTGGGCATGCAGACCCTCGACTCGTGCCTC
AAGGGGCTGCTCGCCAAAGGGCTGATCTCGCGAGACAGTGCCAAGGAAAAGGCCAAGCAGCCAGAGAACTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1I3QZE2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

99.419

100

0.994
  pilT Pseudomonas aeruginosa PAK

94.186

100

0.942
  pilT Acinetobacter baumannii D1279779

84.012

100

0.84
  pilT Acinetobacter baumannii strain A118

84.012

100

0.84
  pilT Acinetobacter nosocomialis M2

83.721

100

0.837
  pilT Acinetobacter baylyi ADP1

83.43

100

0.834
  pilT Legionella pneumophila strain Lp02

74.26

98.256

0.73
  pilT Legionella pneumophila strain ERS1305867

74.26

98.256

0.73
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.139

97.965

0.677
  pilT Vibrio cholerae strain A1552

69.139

97.965

0.677
  pilT Neisseria meningitidis 8013

66.957

100

0.672
  pilT Neisseria gonorrhoeae MS11

66.667

100

0.669
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.754

100

0.529
  pilU Vibrio cholerae strain A1552

41.003

98.547

0.404
  pilU Pseudomonas stutzeri DSM 10701

40.896

97.384

0.398
  pilU Acinetobacter baylyi ADP1

39.306

100

0.395