Detailed information NTG0641714

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: LW136_RS01465
• Genome accession: NZ_CP090366
• Coordinates: 302872..304017 (+)
• Length: 381 a.a.
• NCBI ID: WP_014818747.1
• Uniprot ID: I4CN99
• Organism: Stutzerimonas kunmingensis strain 7850S
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 297872..309017

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LW136_RS01445 (LW136_01445)	-	299547..300137 (-)	591	WP_014818744.1	YggT family protein	-
LW136_RS01450 (LW136_01450)	proC	300188..301012 (-)	825	WP_234174505.1	pyrroline-5-carboxylate reductase	-
LW136_RS01455 (LW136_01455)	-	301023..301712 (-)	690	WP_234174506.1	YggS family pyridoxal phosphate-dependent enzyme	-
LW136_RS01460 (LW136_01460)	pilT	301777..302811 (+)	1035	WP_003303153.1	type IV pilus twitching motility protein PilT	Machinery gene
LW136_RS01465 (LW136_01465)	pilU	302872..304017 (+)	1146	WP_014818747.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
LW136_RS01470 (LW136_01470)	-	304014..304850 (-)	837	WP_234174507.1	TIGR03915 family putative DNA repair protein	-
LW136_RS01475 (LW136_01475)	-	304850..306061 (-)	1212	WP_102832393.1	putative DNA modification/repair radical SAM protein	-
LW136_RS01480 (LW136_01480)	-	306195..306590 (+)	396	WP_003282737.1	NINE protein	-
LW136_RS01485 (LW136_01485)	-	306668..307942 (-)	1275	WP_046617518.1	dihydroorotase	-
LW136_RS01490 (LW136_01490)	-	307942..308958 (-)	1017	WP_023446073.1	aspartate carbamoyltransferase catalytic subunit	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 42457.70 Da
• Isoelectric Point: 6.7256

>NTDB_id=641714 LW136_RS01465 WP_014818747.1 302872..304017(+) (pilU) [Stutzerimonas kunmingensis strain 7850S]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGKIMPVSKTAMSPEMTRETVHGVMNEQQRREFTENHECNFAISARGIGR
FRVSAFYQRNLAGMVLRRIETNIPTIEELKLPDVLKKLSMTKRGLVLFVGATGTGKSTSLASMIGYRNKNSSGHIISIED
PIEFIHQHQNCIVTQREVGIDTSSFEVALKNTLRQAPDVILIGEIRTRETMDYAVAFAETGHLCLATLHANNANQALDRI
INFFPPDRHNQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLINTPLAADLIRKGEVHELKSLMKRSTELGMQTFDQA
LYNLYVQGEITYEDALLHADSANDLRLLIKLGSETDGEHLTSVSQGLSLEVSDDDPGRSFR

Nucleotide

• Length: 1146 bp

>NTDB_id=641714 LW136_RS01465 WP_014818747.1 302872..304017(+) (pilU) [Stutzerimonas kunmingensis strain 7850S]
ATGGAATTCGAGAAACTTCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGATCTGTTCATCACGGCAGGCGTGCCGCCTTC
GATGAAGGTCAACGGCAAGATCATGCCGGTCAGCAAGACGGCGATGTCGCCGGAAATGACCCGCGAGACCGTGCACGGCG
TTATGAACGAGCAGCAGCGCCGCGAGTTCACCGAGAACCATGAGTGCAACTTCGCCATCAGCGCTCGTGGTATCGGCCGT
TTCCGGGTCAGTGCGTTCTACCAGCGCAATCTCGCTGGCATGGTGCTGCGGCGTATCGAAACCAACATTCCGACCATCGA
AGAACTCAAGCTGCCGGACGTCCTCAAGAAGCTGTCGATGACCAAGCGCGGGCTGGTGCTGTTCGTCGGGGCCACCGGTA
CCGGCAAGTCGACCTCATTGGCTTCGATGATCGGCTACCGCAACAAGAATTCCAGCGGGCACATCATCTCCATCGAAGAC
CCGATCGAATTCATCCACCAGCATCAGAACTGCATCGTTACCCAGCGCGAGGTCGGTATCGACACCAGCTCGTTCGAAGT
AGCGCTGAAGAACACCTTGCGCCAGGCGCCGGACGTGATCCTCATCGGCGAGATCCGTACCCGTGAAACCATGGACTACG
CCGTGGCCTTTGCCGAAACCGGTCACCTGTGCCTGGCGACCCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTCCCGCCCGACCGTCACAACCAGGTGTGGATGGATCTGTCGCTCAACCTTAAGGCCATCGTCGCCCAGCA
GCTGGTGCCGACACCTGATGGCAAGGGGCGTCGTGCGGTGATCGAGGTGCTGATCAATACGCCGCTGGCGGCCGACCTGA
TCCGCAAGGGCGAGGTGCATGAGCTCAAGTCGCTGATGAAGCGCTCCACCGAGCTGGGCATGCAGACCTTCGACCAGGCG
CTGTACAACCTCTATGTGCAGGGTGAAATCACCTACGAAGATGCGTTGCTGCACGCCGATTCGGCCAACGACCTGCGTCT
GCTGATCAAGCTGGGATCGGAAACCGATGGCGAGCATCTGACCAGCGTGTCCCAGGGCCTGAGCCTTGAGGTCAGTGACG
ACGATCCAGGCCGCAGCTTCCGCTAG

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (113-270)

3D structure

• AlphaFold DB: I4CN99

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	95.276	100	0.953
pilU	Acinetobacter baylyi ADP1	66.106	93.701	0.619
pilU	Vibrio cholerae strain A1552	59.714	91.864	0.549
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.658	88.976	0.388