Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LN341_RS02330 Genome accession   NZ_CP090364
Coordinates   474248..475282 (+) Length   344 a.a.
NCBI ID   WP_046220511.1    Uniprot ID   A0A0F5VEE5
Organism   Photobacterium sp. TLY01     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 469248..480282
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LN341_RS02295 (LN341_02295) hemW 469389..470549 (-) 1161 WP_234203959.1 radical SAM family heme chaperone HemW -
  LN341_RS02300 (LN341_02300) - 470549..471142 (-) 594 WP_234203960.1 XTP/dITP diphosphatase -
  LN341_RS02305 (LN341_02305) - 471247..471675 (-) 429 WP_046220515.1 DUF4426 domain-containing protein -
  LN341_RS02310 (LN341_02310) yggU 471801..472106 (-) 306 WP_370643725.1 DUF167 family protein YggU -
  LN341_RS02315 (LN341_02315) - 472091..472648 (-) 558 WP_046220514.1 YggT family protein -
  LN341_RS02320 (LN341_02320) proC 472658..473476 (-) 819 WP_046220513.1 pyrroline-5-carboxylate reductase -
  LN341_RS02325 (LN341_02325) - 473523..474224 (-) 702 WP_234203961.1 YggS family pyridoxal phosphate-dependent enzyme -
  LN341_RS02330 (LN341_02330) pilT 474248..475282 (+) 1035 WP_046220511.1 type IV pilus twitching motility protein PilT Machinery gene
  LN341_RS02335 (LN341_02335) pilU 475299..476405 (+) 1107 WP_234203962.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LN341_RS02340 (LN341_02340) - 476597..477913 (+) 1317 WP_046220509.1 YjiH family protein -
  LN341_RS02345 (LN341_02345) ruvX 478068..478496 (-) 429 WP_046220508.1 Holliday junction resolvase RuvX -
  LN341_RS02350 (LN341_02350) - 478493..479056 (-) 564 WP_046220507.1 YqgE/AlgH family protein -
  LN341_RS02355 (LN341_02355) gshB 479207..480157 (-) 951 WP_046220506.1 glutathione synthase -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38065.57 Da        Isoelectric Point: 6.3866

>NTDB_id=641689 LN341_RS02330 WP_046220511.1 474248..475282(+) (pilT) [Photobacterium sp. TLY01]
MDITELLDFSVKHNASDLHLSAGVSPMIRVDGDVRPLSIPPLSHEEAHRLITDIMNDGQRREYEERLEVDFSFVLPDVGR
FRVNAFQQSRGCAAVFRTIPQSVPSLETLQVPEVFYQIAQSQRGLVLVTGATGSGKSTTIAAMVDHINTHTHRHILTIED
PVEFVHQSKRCLINQREVHRDTHSFQNALRSALREDPDVIMVGELRDQETIRLALTAAETGHLVLGTLHTSSAAKAIDRI
IDVFPGNDKAMVRSMLSESLRAVIAQSLLKCPGGGRMAAHEIMLATPAIRNLIREDKVAQMYSMIQTGAGMGMQTMEQAV
NRLMAQGLIEPEEAAKVVERNQGL

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=641689 LN341_RS02330 WP_046220511.1 474248..475282(+) (pilT) [Photobacterium sp. TLY01]
ATGGATATCACCGAACTACTGGACTTTAGTGTAAAGCATAACGCCTCCGATCTGCACCTTTCCGCTGGGGTATCGCCAAT
GATTCGGGTAGACGGTGATGTCAGGCCGCTCAGCATACCGCCTCTGAGCCATGAGGAGGCGCACCGGCTGATCACCGATA
TTATGAATGACGGCCAGCGGCGTGAATATGAAGAAAGGCTGGAAGTGGATTTTTCTTTTGTTCTGCCCGATGTCGGACGG
TTTCGGGTCAATGCGTTTCAGCAGTCCCGCGGGTGTGCTGCGGTGTTCCGGACCATTCCCCAGTCTGTGCCCTCACTGGA
AACCTTGCAGGTGCCTGAGGTGTTTTACCAGATTGCCCAGAGTCAGCGTGGGCTGGTGCTGGTTACCGGAGCAACCGGTT
CAGGCAAATCGACCACCATCGCCGCTATGGTGGATCATATTAACACCCACACGCACCGTCATATTCTGACCATTGAAGAT
CCGGTGGAGTTTGTCCATCAAAGTAAGCGCTGCCTGATCAATCAGCGCGAGGTGCACCGGGATACCCACAGTTTCCAGAA
TGCGCTGCGCTCGGCGTTGCGCGAAGATCCCGATGTGATCATGGTGGGTGAATTGCGCGATCAGGAAACCATACGCTTAG
CGCTGACCGCGGCCGAAACCGGTCATCTGGTGCTGGGTACGCTGCACACCAGCTCAGCGGCCAAGGCAATCGACCGGATC
ATTGATGTCTTCCCCGGCAATGATAAAGCTATGGTGCGTTCTATGCTGTCGGAATCACTGCGGGCGGTGATCGCGCAGAG
CCTGCTCAAATGCCCCGGCGGTGGCAGGATGGCGGCCCATGAAATCATGCTGGCAACGCCGGCGATCCGAAACCTGATCC
GGGAAGACAAAGTGGCCCAGATGTATTCCATGATCCAGACCGGCGCCGGGATGGGGATGCAGACAATGGAGCAGGCCGTG
AACCGTCTCATGGCACAGGGGTTAATTGAACCCGAGGAAGCCGCCAAGGTGGTGGAACGTAATCAGGGATTATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0F5VEE5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

75.221

98.547

0.741
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

75.221

98.547

0.741
  pilT Pseudomonas aeruginosa PAK

68.713

99.419

0.683
  pilT Acinetobacter baumannii D1279779

69.76

97.093

0.677
  pilT Acinetobacter baumannii strain A118

69.76

97.093

0.677
  pilT Acinetobacter nosocomialis M2

69.461

97.093

0.674
  pilT Pseudomonas stutzeri DSM 10701

68.862

97.093

0.669
  pilT Acinetobacter baylyi ADP1

69.605

95.64

0.666
  pilT Legionella pneumophila strain Lp02

66.766

97.093

0.648
  pilT Legionella pneumophila strain ERS1305867

66.766

97.093

0.648
  pilT Neisseria meningitidis 8013

63.768

100

0.64
  pilT Neisseria gonorrhoeae MS11

63.478

100

0.637
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.818

95.93

0.497
  pilU Pseudomonas stutzeri DSM 10701

42.687

97.384

0.416
  pilU Vibrio cholerae strain A1552

42.09

97.384

0.41
  pilU Acinetobacter baylyi ADP1

39.941

98.256

0.392