Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   LN341_RS02335 Genome accession   NZ_CP090364
Coordinates   475299..476405 (+) Length   368 a.a.
NCBI ID   WP_234203962.1    Uniprot ID   -
Organism   Photobacterium sp. TLY01     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 470299..481405
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LN341_RS02300 (LN341_02300) - 470549..471142 (-) 594 WP_234203960.1 XTP/dITP diphosphatase -
  LN341_RS02305 (LN341_02305) - 471247..471675 (-) 429 WP_046220515.1 DUF4426 domain-containing protein -
  LN341_RS02310 (LN341_02310) yggU 471801..472106 (-) 306 WP_370643725.1 DUF167 family protein YggU -
  LN341_RS02315 (LN341_02315) - 472091..472648 (-) 558 WP_046220514.1 YggT family protein -
  LN341_RS02320 (LN341_02320) proC 472658..473476 (-) 819 WP_046220513.1 pyrroline-5-carboxylate reductase -
  LN341_RS02325 (LN341_02325) - 473523..474224 (-) 702 WP_234203961.1 YggS family pyridoxal phosphate-dependent enzyme -
  LN341_RS02330 (LN341_02330) pilT 474248..475282 (+) 1035 WP_046220511.1 type IV pilus twitching motility protein PilT Machinery gene
  LN341_RS02335 (LN341_02335) pilU 475299..476405 (+) 1107 WP_234203962.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LN341_RS02340 (LN341_02340) - 476597..477913 (+) 1317 WP_046220509.1 YjiH family protein -
  LN341_RS02345 (LN341_02345) ruvX 478068..478496 (-) 429 WP_046220508.1 Holliday junction resolvase RuvX -
  LN341_RS02350 (LN341_02350) - 478493..479056 (-) 564 WP_046220507.1 YqgE/AlgH family protein -
  LN341_RS02355 (LN341_02355) gshB 479207..480157 (-) 951 WP_046220506.1 glutathione synthase -
  LN341_RS02360 (LN341_02360) rsmE 480173..480904 (-) 732 WP_234203963.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 40740.87 Da        Isoelectric Point: 6.3640

>NTDB_id=641690 LN341_RS02335 WP_234203962.1 475299..476405(+) (pilU) [Photobacterium sp. TLY01]
MNLDFMLCQMTDQQASDLYLTVAAPCQLKINGRLCPLGESLSAEQLMALCQQSMSDEQWQSFVALKEANFALVRGERRFR
ISAFWQREQPGMVIRRIETVIPDMDSLCLPEPMKAMALAKRGLILVVGATGSGKSTSMAALTGHRNRHSSGHILTVEDPI
EFIHQHQQCIVTQREVGLDTASYEVALKNSLRQAPDMILIGEIRNQETMAHALNFAETGHLCMATLHANNANQALERILH
LVPKEQRESLLFDLSLNLKGILAQQLIPDPAGEGRHAVFELLINTPRVSDLIRRGELHELKATMAKGREHGMITFDQSLY
ALLSQGKICENDALSYADSANDLRLMIKTGQPGQSSAGGLDGVTIRFE

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=641690 LN341_RS02335 WP_234203962.1 475299..476405(+) (pilU) [Photobacterium sp. TLY01]
ATGAATTTGGATTTTATGCTCTGCCAGATGACAGACCAGCAGGCGTCCGACCTGTATCTCACCGTGGCTGCGCCCTGCCA
GCTGAAAATCAACGGGCGGCTGTGTCCGCTCGGTGAAAGCCTGTCGGCAGAACAATTGATGGCCTTGTGCCAGCAGTCGA
TGTCGGATGAGCAGTGGCAGAGCTTTGTTGCACTCAAAGAGGCGAACTTTGCGCTGGTACGCGGTGAGCGCCGTTTTCGT
ATCAGTGCCTTCTGGCAGCGCGAGCAGCCGGGCATGGTGATTCGCCGTATTGAAACCGTGATTCCCGACATGGATTCCCT
CTGCCTGCCTGAACCCATGAAGGCGATGGCGCTGGCAAAACGGGGCTTGATCCTGGTGGTCGGAGCGACCGGCTCGGGGA
AATCAACCTCCATGGCGGCGCTGACAGGGCACCGAAACCGTCATAGCAGCGGTCATATTCTAACGGTGGAAGACCCGATA
GAATTTATTCATCAGCACCAGCAATGCATCGTGACCCAGCGTGAGGTCGGTTTAGATACTGCAAGCTATGAAGTGGCACT
GAAAAATTCTTTGCGTCAGGCACCGGACATGATTTTGATTGGTGAAATACGCAATCAGGAAACCATGGCGCATGCGCTTA
ATTTTGCTGAAACCGGCCATCTGTGTATGGCAACCCTGCATGCCAATAACGCCAATCAGGCGCTGGAACGGATACTGCAT
TTAGTGCCCAAAGAGCAGCGGGAGTCGCTGTTATTTGATTTGTCGCTGAACCTGAAAGGGATACTGGCGCAGCAACTGAT
CCCCGATCCCGCCGGAGAGGGTCGTCATGCGGTGTTTGAGTTACTGATCAATACCCCCAGAGTGTCGGATTTGATCCGGC
GTGGCGAGTTGCATGAACTGAAAGCCACCATGGCCAAAGGCCGTGAGCATGGCATGATCACCTTTGATCAGTCGCTGTAT
GCACTGCTCAGCCAGGGAAAAATCTGCGAGAACGATGCGCTCAGCTATGCCGATTCGGCCAATGATTTGCGCCTGATGAT
CAAAACCGGCCAGCCAGGCCAGAGCTCAGCTGGCGGGCTGGACGGGGTCACGATCCGGTTTGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

65.761

100

0.658
  pilU Pseudomonas stutzeri DSM 10701

57.955

95.652

0.554
  pilU Acinetobacter baylyi ADP1

53.39

96.196

0.514
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.463

89.13

0.37