Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LVB77_RS17085 Genome accession   NZ_CP089924
Coordinates   3782107..3783144 (-) Length   345 a.a.
NCBI ID   WP_055903397.1    Uniprot ID   A0A0Q9ER94
Organism   Lysobacter sp. 5GHs7-4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3777107..3788144
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LVB77_RS17080 (LVB77_17080) pilU 3780859..3781989 (-) 1131 WP_232907275.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LVB77_RS17085 (LVB77_17085) pilT 3782107..3783144 (-) 1038 WP_055903397.1 type IV pilus twitching motility protein PilT Machinery gene
  LVB77_RS17090 (LVB77_17090) - 3783375..3784025 (+) 651 WP_232910333.1 YggS family pyridoxal phosphate-dependent enzyme -
  LVB77_RS17095 (LVB77_17095) proC 3784135..3784980 (+) 846 WP_232907276.1 pyrroline-5-carboxylate reductase -
  LVB77_RS17100 (LVB77_17100) - 3785006..3785497 (+) 492 WP_232907277.1 DUF4426 domain-containing protein -
  LVB77_RS17105 (LVB77_17105) - 3785540..3786505 (-) 966 WP_232907278.1 lipase secretion chaperone -
  LVB77_RS17110 (LVB77_17110) - 3786508..3787467 (-) 960 WP_232907279.1 triacylglycerol lipase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38599.37 Da        Isoelectric Point: 6.7810

>NTDB_id=639111 LVB77_RS17085 WP_055903397.1 3782107..3783144(-) (pilT) [Lysobacter sp. 5GHs7-4]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALDHKQVHSLVYDIMSDKQRRDYEEFLEVDFSFEIPGLAR
FRVNAFNQNRGAGAVFRTIPSEVLSLEDLGCPRIFKELIEQPQGLILVTGPTGSGKSTTLAAMIDHINKNEYAHILSVED
PIEFVHTSQKCLINQREVHRDTHGFNEALRSALREDPDYILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTVDRI
IDVFPAGEKPMVRSMLSESLRAVISQALLKKVGGGRTAAWEIMVGIPAVRNLIREDKVAQMYSAIQTGQNHGMMTLDQHL
QDLVKRGMILRPQAREYAKDKRLFD

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=639111 LVB77_RS17085 WP_055903397.1 3782107..3783144(-) (pilT) [Lysobacter sp. 5GHs7-4]
ATGGATATCGCCGAACTGTTGGCGTTTTCGGTCAAGAACAAGGCATCCGACTTGCATCTGTCGGCCGGATTGCCGCCGAT
GATCCGCGTCGATGGCGACGTGCGGCGCATCAATATTCCAGCGCTGGACCATAAGCAGGTCCACTCGCTGGTGTACGACA
TCATGTCGGACAAGCAGCGCCGCGATTACGAAGAATTCCTCGAGGTCGACTTCTCGTTCGAGATCCCCGGCCTGGCCCGC
TTCCGCGTCAACGCGTTCAACCAGAACCGCGGCGCCGGCGCGGTGTTCCGTACCATTCCTTCGGAAGTGCTGTCGCTCGA
GGATCTGGGCTGCCCGCGCATCTTCAAGGAACTGATCGAGCAGCCGCAGGGCCTGATCCTGGTCACCGGCCCGACCGGTT
CGGGCAAGTCGACCACGCTGGCGGCGATGATCGACCACATCAACAAGAACGAGTACGCGCACATCCTGTCGGTGGAAGAC
CCGATCGAGTTCGTGCACACCTCGCAGAAGTGCCTGATCAACCAGCGCGAAGTCCACCGCGACACCCACGGCTTCAACGA
GGCCCTGCGTTCGGCGCTGCGTGAAGACCCCGACTACATCCTGGTCGGCGAGTTGCGCGATCTGGAAACCATCCGCCTGG
CGCTGACCGCGGCCGAGACCGGCCACTTGGTGTTTGGCACCCTGCACACCTCCTCGGCGGCCAAGACCGTGGACCGCATC
ATCGACGTGTTCCCCGCCGGCGAAAAGCCGATGGTGCGCTCGATGCTGTCGGAGTCGTTGCGCGCAGTGATCTCACAGGC
GCTGCTGAAGAAGGTCGGCGGCGGCCGTACCGCGGCCTGGGAAATCATGGTCGGCATCCCGGCGGTGCGTAACCTGATCC
GCGAGGACAAGGTCGCGCAGATGTACTCGGCGATCCAGACCGGTCAGAACCACGGCATGATGACCCTCGACCAGCACCTC
CAGGACCTGGTGAAGCGCGGCATGATCCTGCGCCCGCAGGCCCGCGAGTACGCCAAGGACAAGCGTCTGTTCGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0Q9ER94

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

79.942

99.71

0.797
  pilT Acinetobacter baumannii D1279779

79.942

99.71

0.797
  pilT Acinetobacter baumannii strain A118

79.942

99.71

0.797
  pilT Acinetobacter baylyi ADP1

77.616

99.71

0.774
  pilT Pseudomonas stutzeri DSM 10701

76.453

99.71

0.762
  pilT Pseudomonas aeruginosa PAK

77.286

98.261

0.759
  pilT Legionella pneumophila strain Lp02

74.128

99.71

0.739
  pilT Legionella pneumophila strain ERS1305867

74.128

99.71

0.739
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.909

95.362

0.667
  pilT Vibrio cholerae strain A1552

69.909

95.362

0.667
  pilT Neisseria meningitidis 8013

66.862

98.841

0.661
  pilT Neisseria gonorrhoeae MS11

66.569

98.841

0.658
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.986

100

0.49
  pilU Pseudomonas stutzeri DSM 10701

40.413

98.261

0.397
  pilU Acinetobacter baylyi ADP1

40

98.551

0.394
  pilU Vibrio cholerae strain A1552

39.385

94.203

0.371