Detailed information NTG0639110

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: LVB77_RS17080
• Genome accession: NZ_CP089924
• Coordinates: 3780859..3781989 (-)
• Length: 376 a.a.
• NCBI ID: WP_232907275.1
• Uniprot ID: -
• Organism: Lysobacter sp. 5GHs7-4
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3775859..3786989

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LVB77_RS17070 (LVB77_17070)	-	3776200..3776766 (+)	567	WP_232907273.1	DNA-3-methyladenine glycosylase I	-
LVB77_RS17075 (LVB77_17075)	-	3776798..3780649 (-)	3852	WP_232907274.1	hypothetical protein	-
LVB77_RS17080 (LVB77_17080)	pilU	3780859..3781989 (-)	1131	WP_232907275.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
LVB77_RS17085 (LVB77_17085)	pilT	3782107..3783144 (-)	1038	WP_055903397.1	type IV pilus twitching motility protein PilT	Machinery gene
LVB77_RS17090 (LVB77_17090)	-	3783375..3784025 (+)	651	WP_232910333.1	YggS family pyridoxal phosphate-dependent enzyme	-
LVB77_RS17095 (LVB77_17095)	proC	3784135..3784980 (+)	846	WP_232907276.1	pyrroline-5-carboxylate reductase	-
LVB77_RS17100 (LVB77_17100)	-	3785006..3785497 (+)	492	WP_232907277.1	DUF4426 domain-containing protein	-
LVB77_RS17105 (LVB77_17105)	-	3785540..3786505 (-)	966	WP_232907278.1	lipase secretion chaperone	-

Sequence

Protein

• Length: 376 a.a.
• Molecular weight: 41913.36 Da
• Isoelectric Point: 6.8286

>NTDB_id=639110 LVB77_RS17080 WP_232907275.1 3780859..3781989(-) (pilU) [Lysobacter sp. 5GHs7-4]
MSTIDFTSFLKLMAHQKASDLFITAGMPPSMKVHGKISPITQNPLTPQQSRDLVLNVMNPQQREEFEKTHECNFAIGVTG
VGRFRVSCFYQRNQVGMVLRRIETKIPTVEELNLPPIIKTLAMTKRGIIIFVGATGTGKSTSLAAMIGYRNMNSSGHIIT
IEDPIEFVHKHEGCIITQREVGIDTDSWDAALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLCTLHANNANQAM
DRIINFFPEDRRGQLLMDLSLNLKGVVAQQLIPTPDGKARRVAMEILLGTPLVQDYIRDGEIHKLKEVMKESVQLGMKTF
DQALFELYQAGEISYEDALRYADSQNEVRLRIKLAQGGDARTLAQGLDGVEVAEVR

Nucleotide

• Length: 1131 bp

>NTDB_id=639110 LVB77_RS17080 WP_232907275.1 3780859..3781989(-) (pilU) [Lysobacter sp. 5GHs7-4]
ATGAGCACCATCGACTTCACCTCGTTCCTCAAGCTGATGGCGCACCAGAAGGCCTCGGACTTGTTCATCACCGCCGGCAT
GCCGCCGTCGATGAAGGTCCACGGCAAGATCTCGCCGATCACCCAGAACCCGCTGACCCCGCAGCAGAGCCGCGACCTCG
TGCTCAACGTGATGAACCCTCAGCAGCGCGAGGAGTTCGAAAAGACCCACGAGTGCAACTTCGCCATCGGCGTGACCGGC
GTCGGCCGCTTCCGCGTGAGCTGCTTCTACCAGCGCAACCAGGTCGGCATGGTGCTGCGCCGCATCGAGACCAAGATCCC
GACCGTCGAAGAGCTGAACCTGCCGCCGATCATCAAGACGCTGGCGATGACCAAGCGCGGCATCATCATCTTCGTCGGCG
CCACCGGTACCGGTAAGTCGACCTCGCTGGCGGCGATGATCGGCTACCGCAACATGAATTCCAGCGGCCACATCATCACG
ATCGAAGACCCGATCGAGTTCGTGCACAAGCACGAGGGCTGCATCATCACCCAGCGCGAAGTCGGCATCGACACCGACAG
CTGGGACGCCGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCACCCGCGAGGGCA
TGGACCACGCCATCGCCTTCGCCGAAACCGGCCACCTGGTGCTGTGCACGTTGCACGCCAACAACGCCAACCAGGCCATG
GATCGCATCATCAACTTCTTCCCCGAGGACCGTCGCGGCCAGCTGCTGATGGACCTGTCGCTGAACCTCAAGGGCGTGGT
CGCGCAGCAGCTGATCCCGACCCCGGACGGCAAGGCGCGCCGGGTCGCGATGGAAATCCTGCTGGGCACGCCGCTGGTGC
AGGACTACATCCGCGACGGCGAAATCCACAAGCTCAAGGAAGTGATGAAGGAGTCCGTGCAGCTGGGCATGAAGACCTTC
GATCAGGCCCTGTTCGAGCTCTACCAGGCCGGCGAGATCAGCTACGAGGACGCGCTGCGTTACGCCGACTCGCAGAACGA
AGTGCGCCTGCGCATCAAGCTGGCCCAGGGCGGCGACGCGCGCACTCTGGCCCAGGGCCTGGACGGCGTCGAGGTCGCGG
AAGTGCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (118-272)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	68.12	97.606	0.665
pilU	Acinetobacter baylyi ADP1	64.146	94.947	0.609
pilU	Vibrio cholerae strain A1552	52.809	94.681	0.5
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.143	93.085	0.402
pilT	Acinetobacter baumannii strain A118	40.116	91.489	0.367
pilT	Acinetobacter nosocomialis M2	40.116	91.489	0.367
pilT	Acinetobacter baumannii D1279779	40.116	91.489	0.367
pilT	Pseudomonas aeruginosa PAK	40.896	89.096	0.364