Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LUM42_RS02280 Genome accession   NZ_CP089603
Coordinates   471065..472102 (+) Length   345 a.a.
NCBI ID   WP_004724259.1    Uniprot ID   -
Organism   Vibrio furnissii strain VFN3     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 466065..477102
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LUM42_RS02245 (LUM42_02245) hemW 466293..467465 (-) 1173 WP_158166165.1 radical SAM family heme chaperone HemW -
  LUM42_RS02250 (LUM42_02250) - 467465..468064 (-) 600 WP_232658421.1 XTP/dITP diphosphatase -
  LUM42_RS02255 (LUM42_02255) - 468088..468519 (-) 432 WP_232658422.1 DUF4426 domain-containing protein -
  LUM42_RS02260 (LUM42_02260) yggU 468577..468870 (-) 294 WP_416068940.1 DUF167 family protein YggU -
  LUM42_RS02265 (LUM42_02265) - 468867..469424 (-) 558 WP_004724262.1 YggT family protein -
  LUM42_RS02270 (LUM42_02270) proC 469476..470294 (-) 819 WP_232658423.1 pyrroline-5-carboxylate reductase -
  LUM42_RS02275 (LUM42_02275) - 470332..471039 (-) 708 WP_158108025.1 YggS family pyridoxal phosphate-dependent enzyme -
  LUM42_RS02280 (LUM42_02280) pilT 471065..472102 (+) 1038 WP_004724259.1 type IV pilus twitching motility protein PilT Machinery gene
  LUM42_RS02285 (LUM42_02285) pilU 472114..473220 (+) 1107 WP_004724258.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LUM42_RS02290 (LUM42_02290) ruvX 473317..473742 (-) 426 WP_055467261.1 Holliday junction resolvase RuvX -
  LUM42_RS02295 (LUM42_02295) - 473805..474368 (-) 564 WP_232658424.1 YqgE/AlgH family protein -
  LUM42_RS02300 (LUM42_02300) gshB 474407..475363 (-) 957 WP_004724255.1 glutathione synthase -
  LUM42_RS02305 (LUM42_02305) rsmE 475376..476107 (-) 732 WP_004724254.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  LUM42_RS02310 (LUM42_02310) - 476215..476916 (-) 702 WP_232658425.1 endonuclease -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38233.79 Da        Isoelectric Point: 6.4499

>NTDB_id=638339 LUM42_RS02280 WP_004724259.1 471065..472102(+) (pilT) [Vibrio furnissii strain VFN3]
MDISELLEFSVKHNASDLHLSAGVPPMVRIDGEVRKLGVPAFTHADVHRLIFEIMNDAQRSEYEEKLEVDFSFELPDIGR
FRVNAFHQSRGASAVFRTIPTTIPTLEQLHAPDIFNKIANYEKGLVLVTGPTGSGKSTTLAAMVDYINATHNKHVLTIED
PIEFVHSNNKCLINQREVHRDTHSFKNALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLRAVIAQKLLKRLGGGRVACHEIMLATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNA
KQLIAQGLVDPAEVQKKIELEMSAF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=638339 LUM42_RS02280 WP_004724259.1 471065..472102(+) (pilT) [Vibrio furnissii strain VFN3]
ATGGATATCTCTGAGTTACTGGAATTTAGTGTAAAGCATAATGCGTCAGATCTACATCTTTCTGCGGGCGTTCCTCCGAT
GGTGCGTATTGATGGCGAGGTGAGAAAGCTTGGTGTGCCTGCGTTTACACACGCTGACGTGCATCGATTAATTTTTGAAA
TCATGAATGATGCCCAGCGCAGTGAGTACGAAGAAAAGCTGGAAGTGGACTTTTCCTTTGAATTACCGGATATCGGTCGT
TTTCGTGTCAACGCTTTCCATCAGTCGCGCGGTGCATCCGCGGTTTTTCGGACCATTCCAACGACGATTCCAACGCTAGA
GCAACTGCACGCGCCGGACATCTTCAACAAAATCGCCAATTACGAGAAAGGGCTGGTGTTGGTGACGGGGCCTACGGGCT
CGGGTAAATCGACCACATTGGCCGCGATGGTTGATTACATTAACGCCACCCACAACAAGCATGTGCTGACCATCGAAGAT
CCGATTGAGTTTGTGCACAGCAACAACAAATGTTTGATCAACCAACGTGAAGTGCACCGCGACACGCACAGTTTTAAAAA
TGCGCTGCGCAGTGCGCTGCGTGAAGATCCGGATGTGATTCTGGTCGGTGAGCTGCGCGATCAAGAGACCATCAGCTTAG
CGCTGACGGCGGCGGAAACTGGCCATTTGGTGTTTGGGACGCTGCACACCAGTTCGGCGGCGAAAACCATTGACCGCATC
ATTGACGTGTTTCCGGGCAGTGATAAAGACATGGTGCGTTCGATGTTATCAGAGTCGCTGCGCGCCGTGATTGCGCAGAA
ACTGCTCAAACGTCTCGGCGGTGGGCGGGTGGCGTGTCATGAAATCATGTTGGCGACACCGGCGATTCGTAACTTGATCC
GCGAAGACAAAGTCGCGCAGATGTATTCGATCATTCAGACGGGCGCGGCGCATGGCATGCAAACCATGGAACAGAACGCC
AAGCAGTTGATTGCACAAGGGCTGGTGGATCCGGCGGAAGTGCAGAAGAAAATTGAACTGGAAATGTCCGCATTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

93.333

100

0.933
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

93.333

100

0.933
  pilT Acinetobacter baumannii D1279779

72.205

95.942

0.693
  pilT Acinetobacter baumannii strain A118

72.205

95.942

0.693
  pilT Acinetobacter baylyi ADP1

72.121

95.652

0.69
  pilT Acinetobacter nosocomialis M2

71.903

95.942

0.69
  pilT Pseudomonas stutzeri DSM 10701

68.843

97.681

0.672
  pilT Pseudomonas aeruginosa PAK

67.059

98.551

0.661
  pilT Legionella pneumophila strain Lp02

67.781

95.362

0.646
  pilT Legionella pneumophila strain ERS1305867

67.781

95.362

0.646
  pilT Neisseria meningitidis 8013

63.235

98.551

0.623
  pilT Neisseria gonorrhoeae MS11

62.941

98.551

0.62
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.774

92.174

0.496
  pilU Pseudomonas stutzeri DSM 10701

42.47

96.232

0.409
  pilU Vibrio cholerae strain A1552

42.769

94.203

0.403
  pilU Acinetobacter baylyi ADP1

40.312

92.754

0.374