Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   LUM42_RS02285 Genome accession   NZ_CP089603
Coordinates   472114..473220 (+) Length   368 a.a.
NCBI ID   WP_004724258.1    Uniprot ID   -
Organism   Vibrio furnissii strain VFN3     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 467114..478220
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LUM42_RS02250 (LUM42_02250) - 467465..468064 (-) 600 WP_232658421.1 XTP/dITP diphosphatase -
  LUM42_RS02255 (LUM42_02255) - 468088..468519 (-) 432 WP_232658422.1 DUF4426 domain-containing protein -
  LUM42_RS02260 (LUM42_02260) yggU 468577..468870 (-) 294 WP_416068940.1 DUF167 family protein YggU -
  LUM42_RS02265 (LUM42_02265) - 468867..469424 (-) 558 WP_004724262.1 YggT family protein -
  LUM42_RS02270 (LUM42_02270) proC 469476..470294 (-) 819 WP_232658423.1 pyrroline-5-carboxylate reductase -
  LUM42_RS02275 (LUM42_02275) - 470332..471039 (-) 708 WP_158108025.1 YggS family pyridoxal phosphate-dependent enzyme -
  LUM42_RS02280 (LUM42_02280) pilT 471065..472102 (+) 1038 WP_004724259.1 type IV pilus twitching motility protein PilT Machinery gene
  LUM42_RS02285 (LUM42_02285) pilU 472114..473220 (+) 1107 WP_004724258.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LUM42_RS02290 (LUM42_02290) ruvX 473317..473742 (-) 426 WP_055467261.1 Holliday junction resolvase RuvX -
  LUM42_RS02295 (LUM42_02295) - 473805..474368 (-) 564 WP_232658424.1 YqgE/AlgH family protein -
  LUM42_RS02300 (LUM42_02300) gshB 474407..475363 (-) 957 WP_004724255.1 glutathione synthase -
  LUM42_RS02305 (LUM42_02305) rsmE 475376..476107 (-) 732 WP_004724254.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  LUM42_RS02310 (LUM42_02310) - 476215..476916 (-) 702 WP_232658425.1 endonuclease -
  LUM42_RS02315 (LUM42_02315) - 477055..477555 (-) 501 WP_014205820.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41357.36 Da        Isoelectric Point: 6.4509

>NTDB_id=638340 LUM42_RS02285 WP_004724258.1 472114..473220(+) (pilU) [Vibrio furnissii strain VFN3]
MQLEECLQGMMTHKASDLYITVGAPVLYRVDGELRAHGDALNEAQVRTLLAAMMDDTRHADYLATREANFAIVRDLGRFR
VSAFFQRELPGAVIRRIETQIPTFDELKLPPVLKDLSIAKRGLVLVVGATGSGKSTTMAAMTGYRNMHRTGHILTVEDPI
EFVHEHQRCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKEQREQFLFDLSMNLKGVVGQQLIRDKNGQGRHGVFEVLLNSPRVSDIIRRGELHELKNTMAKSKEVGMQTFDQALY
QLVVEHKISEQDALHSADSANDLRLMLKTQRGDVTSSGSLDHVQIDME

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=638340 LUM42_RS02285 WP_004724258.1 472114..473220(+) (pilU) [Vibrio furnissii strain VFN3]
ATGCAACTTGAGGAGTGCCTGCAAGGCATGATGACGCATAAAGCGTCGGATCTGTATATCACTGTGGGCGCGCCCGTGTT
GTATCGGGTCGATGGTGAACTGCGCGCCCACGGCGACGCGCTGAATGAAGCGCAGGTCAGGACGCTGTTGGCCGCGATGA
TGGATGACACGCGCCATGCCGACTACTTGGCGACGCGTGAAGCCAACTTTGCCATTGTGCGTGATTTAGGGCGATTTCGC
GTTAGCGCTTTTTTTCAGCGCGAGCTGCCCGGCGCAGTGATCCGCCGTATTGAAACGCAAATTCCCACGTTTGATGAACT
CAAACTGCCACCCGTGTTGAAAGATCTCTCGATTGCCAAACGCGGTTTGGTGTTGGTGGTGGGGGCGACTGGATCGGGGA
AATCGACCACCATGGCCGCCATGACCGGGTATCGCAATATGCATCGCACCGGGCATATTCTCACCGTTGAAGATCCGATT
GAATTTGTCCATGAACATCAGCGCTGCATCGTGACGCAGCGTGAAGTGGGGTTGGACACGGAAAGTTATGAAGTGGCGCT
GAAGAATTCGCTGCGTCAGGCACCGGACATGATTTTGATCGGCGAGATCCGCAGTCGGGAAACCATGGAATACGCGATGA
CGTTTGCCGAAACGGGGCATTTGTGCATGGCCACTTTGCACGCCAACAACGCCAACCAAGCGCTTGAGCGGATTTTACAT
CTGGTGCCGAAAGAGCAGCGTGAGCAATTTCTGTTTGATTTGTCGATGAACCTCAAAGGGGTTGTCGGCCAGCAACTGAT
CCGCGATAAAAACGGCCAAGGGCGTCACGGGGTGTTTGAAGTGCTGCTCAACAGCCCGCGGGTGTCGGACATCATTCGTC
GCGGCGAGCTGCATGAGTTGAAAAACACCATGGCGAAATCAAAAGAAGTCGGCATGCAGACGTTTGATCAGGCGCTGTAT
CAGTTGGTGGTTGAGCACAAGATCAGCGAGCAAGATGCGCTGCACAGTGCGGATTCGGCCAATGATCTGCGCTTGATGCT
CAAGACGCAGCGGGGCGATGTGACCAGCAGCGGGTCGCTGGATCATGTGCAAATCGACATGGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(73-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

85.87

100

0.859
  pilU Pseudomonas stutzeri DSM 10701

57.143

95.109

0.543
  pilU Acinetobacter baylyi ADP1

54.416

95.38

0.519
  pilT Acinetobacter baylyi ADP1

41.642

92.663

0.386
  pilT Legionella pneumophila strain Lp02

41.593

92.12

0.383
  pilT Legionella pneumophila strain ERS1305867

41.593

92.12

0.383
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.077

88.315

0.38
  pilT Acinetobacter baumannii D1279779

41.003

92.12

0.378
  pilT Acinetobacter baumannii strain A118

41.003

92.12

0.378
  pilT Acinetobacter nosocomialis M2

41.003

92.12

0.378
  pilT Pseudomonas aeruginosa PAK

40.708

92.12

0.375
  pilT Pseudomonas stutzeri DSM 10701

39.233

92.12

0.361