Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   DYST_RS10000 Genome accession   NZ_CP089414
Coordinates   2327766..2328806 (-) Length   346 a.a.
NCBI ID   WP_102302101.1    Uniprot ID   -
Organism   Dyella terrae strain Ely Copper Mine     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2322766..2333806
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DYST_RS09980 (DYST_02044) - 2324014..2324757 (-) 744 WP_239951631.1 MHYT domain-containing protein -
  DYST_RS09985 (DYST_02045) - 2324822..2325628 (-) 807 WP_239951633.1 hypothetical protein -
  DYST_RS09990 - 2326315..2326440 (+) 126 WP_239951634.1 BON domain-containing protein -
  DYST_RS09995 (DYST_02047) pilU 2326529..2327695 (-) 1167 WP_102302102.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  DYST_RS10000 (DYST_02048) pilT 2327766..2328806 (-) 1041 WP_102302101.1 type IV pilus twitching motility protein PilT Machinery gene
  DYST_RS10005 (DYST_02049) proC 2329118..2329942 (+) 825 WP_239951635.1 pyrroline-5-carboxylate reductase -
  DYST_RS10010 (DYST_02050) - 2329939..2330514 (+) 576 WP_239951636.1 YggT family protein -
  DYST_RS24000 (DYST_02051) - 2330610..2331317 (+) 708 WP_275666956.1 hypothetical protein -
  DYST_RS24005 (DYST_02052) - 2331269..2332681 (+) 1413 WP_275666957.1 M13 family metallopeptidase -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38511.03 Da        Isoelectric Point: 6.7293

>NTDB_id=636862 DYST_RS10000 WP_102302101.1 2327766..2328806(-) (pilT) [Dyella terrae strain Ely Copper Mine]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALDHKQVHSLIYDIMSDKQRRDYEEFLEVDFSFEIPGLAR
FRVNAFNQNRGSGGVFRTIPSEVLTLEDLAAPSIFRELIEQPQGLILVTGPTGSGKSTTLAAMVDHINKNEYGHILTIED
PIEFVHTSQKCLINQREVHRDTHGFNEALRSALREDPDYVLVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTVDRI
IDVFPAGEKPMVRSMLSESLRAVISQSLLKKVGGGRIAAHEIMVGIPAIRNLIREDKVAQMYSSIQTGQQYGMQTLDQNL
QDLVKRGLVTRQQASSYARNKDIFKG

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=636862 DYST_RS10000 WP_102302101.1 2327766..2328806(-) (pilT) [Dyella terrae strain Ely Copper Mine]
ATGGATATCGCCGAACTGCTTGCCTTCTCGGTGAAGAACAAGGCCTCGGACTTACACTTGTCCGCCGGTCTGCCACCGAT
GATCCGCGTGGACGGCGACGTTCGCCGCATCAACATCCCCGCGCTGGACCACAAGCAGGTCCATTCGCTGATCTACGACA
TCATGTCGGACAAGCAGCGACGCGACTACGAAGAATTTCTCGAGGTCGACTTTTCGTTCGAAATTCCCGGGCTGGCGCGT
TTTCGTGTCAATGCGTTCAACCAGAACCGTGGCTCGGGCGGCGTGTTCCGTACCATTCCCTCGGAAGTGCTCACGCTGGA
AGACCTTGCGGCACCGTCCATATTCCGCGAACTGATCGAGCAGCCGCAGGGCCTGATCCTGGTGACCGGTCCGACGGGTT
CGGGTAAGTCGACCACGCTGGCGGCGATGGTGGATCACATCAACAAGAACGAATACGGCCACATCCTCACCATCGAGGAT
CCGATCGAATTCGTGCATACCTCGCAGAAGTGCCTGATCAACCAGCGCGAAGTGCACCGCGACACGCACGGCTTCAACGA
AGCCTTGCGCTCGGCGCTCCGCGAAGATCCGGACTATGTGCTGGTGGGTGAGTTGCGCGACTTGGAAACCATCCGCCTGG
CGTTGACCGCCGCGGAAACCGGACACTTGGTGTTCGGTACGCTGCACACCAGTTCGGCGGCCAAGACCGTCGACCGTATC
ATCGACGTGTTCCCTGCCGGCGAAAAGCCGATGGTGCGCTCGATGCTGTCCGAATCGTTGCGCGCTGTGATCTCGCAGTC
GCTGCTGAAGAAAGTGGGCGGTGGTCGTATCGCGGCGCACGAGATCATGGTGGGCATCCCGGCCATCCGCAACCTGATTC
GCGAGGACAAGGTGGCGCAGATGTATTCGTCCATCCAGACTGGCCAGCAGTACGGCATGCAGACGCTCGACCAGAACTTG
CAGGACCTGGTGAAGCGTGGCCTGGTGACGCGTCAGCAGGCATCGAGTTACGCGCGCAACAAGGACATCTTTAAGGGCTA
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

77.616

99.422

0.772
  pilT Acinetobacter baumannii strain A118

77.616

99.422

0.772
  pilT Acinetobacter nosocomialis M2

77.616

99.422

0.772
  pilT Pseudomonas stutzeri DSM 10701

77.326

99.422

0.769
  pilT Acinetobacter baylyi ADP1

76.744

99.422

0.763
  pilT Pseudomonas aeruginosa PAK

75.872

99.422

0.754
  pilT Legionella pneumophila strain Lp02

72.674

99.422

0.723
  pilT Legionella pneumophila strain ERS1305867

72.674

99.422

0.723
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.429

97.11

0.694
  pilT Vibrio cholerae strain A1552

71.429

97.11

0.694
  pilT Neisseria meningitidis 8013

66.181

99.133

0.656
  pilT Neisseria gonorrhoeae MS11

65.889

99.133

0.653
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.151

95.665

0.48
  pilU Acinetobacter baylyi ADP1

40

98.266

0.393
  pilU Pseudomonas stutzeri DSM 10701

39.823

97.977

0.39
  pilU Vibrio cholerae strain A1552

39.403

96.821

0.382