Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LLE28_RS10215 Genome accession   NZ_CP106771
Coordinates   1947556..1948599 (+) Length   347 a.a.
NCBI ID   WP_229930592.1    Uniprot ID   -
Organism   Neisseria gonorrhoeae strain 10531     
Function   pseudopilus biogenesis (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1942556..1953599
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LLE28_RS10190 - 1944789..1944923 (-) 135 WP_258076284.1 hypothetical protein -
  LLE28_RS10195 dksA 1944938..1945354 (-) 417 WP_003692108.1 RNA polymerase-binding protein DksA -
  LLE28_RS10200 proC 1945503..1946294 (-) 792 WP_003694504.1 pyrroline-5-carboxylate reductase -
  LLE28_RS10205 - 1946343..1946750 (-) 408 WP_003690202.1 hypothetical protein -
  LLE28_RS10210 - 1946750..1947436 (-) 687 WP_003692103.1 YggS family pyridoxal phosphate-dependent enzyme -
  LLE28_RS10215 pilT 1947556..1948599 (+) 1044 WP_229930592.1 type IV pilus twitching motility protein PilT Machinery gene
  LLE28_RS10220 pilU 1948763..1949989 (+) 1227 WP_003692100.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LLE28_RS10225 yccS 1950106..1952256 (+) 2151 WP_003692098.1 YccS family putative transporter -
  LLE28_RS10230 - 1952738..1953022 (-) 285 WP_003692093.1 GIY-YIG nuclease family protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 37956.36 Da        Isoelectric Point: 6.8962

>NTDB_id=636737 LLE28_RS10215 WP_229930592.1 1947556..1948599(+) (pilT) [Neisseria gonorrhoeae strain 10531]
MQITDLLAFGAKNKASDLHLSSGISPMIRVHGDMRRINLPEMSAEEVGNMVTSVMNDHQRKIYQQNLEVDFSFELLNVAR
FRVNAFNTGRGPAAVFRTIPSTVLSLEELKAPSIFQKIAESPRGMVLVTGPTGSGKSTTLAAMINYINETQPAHILTIED
PIEFVHQSKKSLINQRELHQHTLSFANALSSALREDPDVILVGEMRDPETIGLALTAAETGHLVFGTLHTTGAAKTVDRI
VDVFPAGEKEMVRSMLSESLTAVISQNLLKTHDGNGRVASHEILIANPAVRNLIRENKITQINSVLQTGQASGMQTMDQS
LQSLVRQGLIAPEAARRRAQNSESMSF

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=636737 LLE28_RS10215 WP_229930592.1 1947556..1948599(+) (pilT) [Neisseria gonorrhoeae strain 10531]
ATGCAGATTACCGACTTACTCGCCTTCGGCGCTAAAAACAAAGCATCCGACCTTCACCTGAGTTCGGGCATATCCCCTAT
GATTCGGGTTCACGGCGACATGCGGCGCATCAACCTTCCCGAAATGAGCGCGGAAGAGGTCGGCAATATGGTAACTTCGG
TGATGAACGACCACCAGCGGAAAATCTACCAGCAAAACTTGGAAGTCGACTTCTCGTTCGAACTGCTCAACGTCGCCCGA
TTCCGCGTCAACGCCTTCAACACCGGCCGCGGCCCCGCCGCCGTATTCCGCACCATTCCCAGCACCGTCTTATCGCTGGA
AGAATTGAAAGCCCCGAGCATTTTCCAAAAAATCGCAGAATCGCCGCGCGGCATGGTATTGGTTACCGGCCCTACCGGTT
CGGGCAAATCGACCACGCTTGCCGCGATGATCAACTACATCAACGAAACCCAGCCGGCACACATCCTGACCATCGAAGAC
CCGATCGAATTCGTCCACCAAAGCAAAAAATCCCTGATTAACCAACGCGAGCTGCACCAGCACACCCTCAGCTTCGCCAA
CGCGCTGAGTTCCGCATTGCGCGAAGACCCCGACGTTATCCTTGTCGGCGAGATGCGCGACCCCGAAACCATCGGCTTGG
CACTGACCGCCGCCGAAACCGGACACTTGGTTTTCGGCACGCTGCACACGACCGGCGCGGCAAAAACCGTCGACCGTATC
GTGGACGTATTCCCCGCCGGAGAGAAAGAAATGGTGCGTTCCATGCTGTCCGAATCGCTGACCGCCGTCATCTCCCAAAA
CCTGCTGAAAACGCACGACGGCAACGGCCGTGTCGCCTCGCACGAAATCCTGATTGCCAACCCCGCCGTCCGCAACCTCA
TCCGCGAAAACAAAATCACGCAGATTAACTCCGTCCTGCAAACCGGGCAGGCGAGCGGTATGCAGACGATGGACCAATCG
CTGCAATCGCTGGTGCGCCAAGGGCTGATCGCACCGGAAGCCGCACGCAGACGCGCGCAAAACAGCGAAAGTATGAGTTT
CTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(12-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Neisseria gonorrhoeae MS11

99.712

100

0.997
  pilT Neisseria meningitidis 8013

98.847

100

0.988
  pilT Acinetobacter baumannii D1279779

67.536

99.424

0.671
  pilT Acinetobacter baumannii strain A118

67.536

99.424

0.671
  pilT Acinetobacter nosocomialis M2

67.536

99.424

0.671
  pilT Acinetobacter baylyi ADP1

66.957

99.424

0.666
  pilT Pseudomonas stutzeri DSM 10701

66.667

99.424

0.663
  pilT Pseudomonas aeruginosa PAK

66.087

99.424

0.657
  pilT Legionella pneumophila strain Lp02

64.516

98.271

0.634
  pilT Legionella pneumophila strain ERS1305867

64.516

98.271

0.634
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

63.939

95.101

0.608
  pilT Vibrio cholerae strain A1552

63.939

95.101

0.608
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.392

97.695

0.522
  pilU Pseudomonas stutzeri DSM 10701

40.29

99.424

0.401
  pilU Acinetobacter baylyi ADP1

38.793

100

0.389
  pilU Vibrio cholerae strain A1552

38.329

100

0.383