Detailed information NTG0636738

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: LLE28_RS10220
• Genome accession: NZ_CP106771
• Coordinates: 1948763..1949989 (+)
• Length: 408 a.a.
• NCBI ID: WP_003692100.1
• Uniprot ID: Q5F5L4
• Organism: Neisseria gonorrhoeae strain 10531
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1943763..1954989

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LLE28_RS10190	-	1944789..1944923 (-)	135	WP_258076284.1	hypothetical protein	-
LLE28_RS10195	dksA	1944938..1945354 (-)	417	WP_003692108.1	RNA polymerase-binding protein DksA	-
LLE28_RS10200	proC	1945503..1946294 (-)	792	WP_003694504.1	pyrroline-5-carboxylate reductase	-
LLE28_RS10205	-	1946343..1946750 (-)	408	WP_003690202.1	hypothetical protein	-
LLE28_RS10210	-	1946750..1947436 (-)	687	WP_003692103.1	YggS family pyridoxal phosphate-dependent enzyme	-
LLE28_RS10215	pilT	1947556..1948599 (+)	1044	WP_229930592.1	type IV pilus twitching motility protein PilT	Machinery gene
LLE28_RS10220	pilU	1948763..1949989 (+)	1227	WP_003692100.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
LLE28_RS10225	yccS	1950106..1952256 (+)	2151	WP_003692098.1	YccS family putative transporter	-
LLE28_RS10230	-	1952738..1953022 (-)	285	WP_003692093.1	GIY-YIG nuclease family protein	-

Sequence

Protein

• Length: 408 a.a.
• Molecular weight: 45698.33 Da
• Isoelectric Point: 7.2909

>NTDB_id=636738 LLE28_RS10220 WP_003692100.1 1948763..1949989(+) (pilU) [Neisseria gonorrhoeae strain 10531]
MNTDNLHDILDETVQVYSQKKQSRSETPAEIGTHFHPLLDRLCETAEAQNASDILISKGFPPSLKINSALTPQPQKALTG
EETAAIAASTMNAEQSEIFRRDGEINYSVQSRSGTRYRANAYYSQGSAGLVLRRINHVIPQMRELGLPEKLKDLAVAPRG
LLIIVGPTGSGKSTTMATMLEHRNKTLPGHIVTIEDPIEFIYKPRRCIFTQREIGVDTINWQTAVQNAMRQSPDVVCIGE
VRSRESMEYAMQLAQTGHLCIFTLHANTAPQSLERILNFYPKEQHNQILIDIALNLTGIICQRLALKKDKTGRTAVVDLL
INTPAIQDFILKGDLMNISKIMETAKTDGMQTMDQNLFELYRHGIISYEEALRQSVSANNLRLHIQLHKEGKTPELLYDR
VNGLNLIS

Nucleotide

• Length: 1227 bp

>NTDB_id=636738 LLE28_RS10220 WP_003692100.1 1948763..1949989(+) (pilU) [Neisseria gonorrhoeae strain 10531]
ATGAATACCGATAACCTGCACGACATCTTGGACGAAACGGTTCAAGTGTATTCTCAAAAAAAACAAAGCCGATCCGAAAC
CCCGGCCGAAATCGGCACACACTTCCACCCGCTGCTCGACCGCCTGTGCGAAACCGCAGAAGCACAAAACGCGTCCGACA
TCCTTATCAGCAAAGGATTCCCGCCCTCGTTGAAAATCAACAGCGCATTAACCCCGCAGCCGCAAAAGGCTCTGACGGGC
GAGGAAACCGCCGCCATCGCCGCATCGACGATGAACGCCGAACAATCGGAAATATTCCGGCGCGACGGCGAAATCAACTA
CTCCGTCCAATCGCGCAGCGGCACGCGCTACCGCGCCAACGCCTACTACAGCCAAGGCAGCGCAGGTTTGGTTTTGCGGC
GCATCAACCACGTCATCCCGCAAATGCGGGAATTGGGCTTGCCCGAAAAACTCAAAGACCTCGCCGTCGCACCGCGCGGG
CTGCTGATTATCGTCGGGCCTACCGGTTCGGGCAAATCCACCACGATGGCGACTATGCTCGAACACCGCAACAAAACCCT
GCCCGGCCATATCGTTACCATCGAAGACCCGATTGAGTTTATCTACAAACCGCGCCGCTGCATCTTTACCCAGCGCGAAA
TCGGCGTCGACACCATAAACTGGCAGACGGCGGTACAAAACGCTATGCGCCAATCCCCAGACGTGGTCTGTATCGGCGAA
GTCCGCAGCAGGGAAAGTATGGAATACGCGATGCAGCTCGCCCAAACCGGCCACCTGTGCATTTTTACGCTCCACGCCAA
CACCGCGCCGCAGTCGCTCGAACGCATACTCAACTTCTACCCCAAAGAACAGCACAACCAAATACTGATTGACATCGCCC
TCAATCTGACCGGCATCATCTGCCAACGCCTCGCCCTCAAAAAAGACAAAACGGGCAGGACGGCGGTTGTCGACTTGCTC
ATCAACACGCCCGCCATCCAGGACTTCATCCTGAAGGGCGACCTGATGAACATCAGTAAAATCATGGAAACCGCCAAAAC
CGACGGAATGCAGACGATGGACCAAAACCTTTTCGAACTGTACCGTCACGGCATCATCAGTTACGAAGAAGCCCTGCGCC
AGTCCGTTTCCGCCAACAACCTGCGATTGCACATCCAACTGCACAAAGAAGGCAAAACGCCCGAACTCCTTTACGACAGG
GTCAACGGTCTCAACCTCATTTCCTGA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (49-305)

3D structure

• AlphaFold DB: Q5F5L4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	44.875	88.48	0.397
pilU	Pseudomonas stutzeri DSM 10701	45.225	87.255	0.395
pilU	Vibrio cholerae strain A1552	47.024	82.353	0.387