Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LTQ02_RS11445 Genome accession   NZ_CP089205
Coordinates   2559222..2560259 (-) Length   345 a.a.
NCBI ID   WP_004735478.1    Uniprot ID   A0A1R3EAA6
Organism   Vibrio splendidus strain 4_C08b     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2554222..2565259
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LTQ02_RS11415 (LTQ02_11415) rsmE 2554908..2555639 (+) 732 WP_017077778.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  LTQ02_RS11420 (LTQ02_11420) gshB 2555649..2556599 (+) 951 WP_004735482.1 glutathione synthase -
  LTQ02_RS11425 (LTQ02_11425) - 2556716..2557279 (+) 564 WP_017095267.1 YqgE/AlgH family protein -
  LTQ02_RS11430 (LTQ02_11430) ruvX 2557357..2557779 (+) 423 WP_004735480.1 Holliday junction resolvase RuvX -
  LTQ02_RS11435 (LTQ02_11435) - 2557878..2558039 (+) 162 WP_243578769.1 hypothetical protein -
  LTQ02_RS11440 (LTQ02_11440) pilU 2558098..2559207 (-) 1110 WP_004735479.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LTQ02_RS11445 (LTQ02_11445) pilT 2559222..2560259 (-) 1038 WP_004735478.1 type IV pilus twitching motility protein PilT Machinery gene
  LTQ02_RS11450 (LTQ02_11450) - 2560286..2560990 (+) 705 WP_243578768.1 YggS family pyridoxal phosphate-dependent enzyme -
  LTQ02_RS11455 (LTQ02_11455) proC 2561075..2561893 (+) 819 WP_108167423.1 pyrroline-5-carboxylate reductase -
  LTQ02_RS11460 (LTQ02_11460) - 2561969..2562526 (+) 558 WP_004735475.1 YggT family protein -
  LTQ02_RS11465 (LTQ02_11465) yggU 2562556..2562846 (+) 291 WP_243578767.1 DUF167 family protein YggU -
  LTQ02_RS11470 (LTQ02_11470) - 2562875..2563306 (+) 432 WP_004735472.1 DUF4426 domain-containing protein -
  LTQ02_RS11475 (LTQ02_11475) - 2563356..2563957 (+) 602 Protein_2241 XTP/dITP diphosphatase -
  LTQ02_RS11480 (LTQ02_11480) hemW 2563957..2565144 (+) 1188 WP_243585237.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38317.83 Da        Isoelectric Point: 6.0512

>NTDB_id=635755 LTQ02_RS11445 WP_004735478.1 2559222..2560259(-) (pilT) [Vibrio splendidus strain 4_C08b]
MDITELLDFSVKHNASDLHLSAGVSPMVRIDGEVRKLGIPALSHADVHRLVFEIMSDSQRGEFEEKLEVDFSFELPNVGR
FRVNAFNQSRGCSAVFRTIPVEIPTLEQLGAPEIFERISNYEKGLVLVTGPTGSGKSTTLAAMVDYVNRNHNKHILTIED
PIEFVHTNNKCLINQREVHRDTHSFKAALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLRSVIAQKLLKRVGGGRVACHEIMMATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNA
KQLMAQGLVDSEEVEKKIEIETSMF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=635755 LTQ02_RS11445 WP_004735478.1 2559222..2560259(-) (pilT) [Vibrio splendidus strain 4_C08b]
ATGGATATCACTGAGTTACTAGATTTTAGTGTAAAGCATAACGCGTCAGATCTACATCTTTCTGCGGGTGTATCTCCAAT
GGTACGTATAGATGGTGAAGTAAGGAAGCTTGGAATACCAGCTTTGAGTCATGCTGATGTGCATCGTTTGGTTTTTGAGA
TCATGAGTGATTCACAACGCGGTGAATTTGAAGAAAAACTGGAAGTCGACTTCTCTTTTGAATTACCCAACGTTGGTCGT
TTCCGTGTTAATGCTTTTAACCAATCTCGTGGCTGCTCTGCTGTGTTCCGTACCATTCCAGTAGAGATTCCCACCCTTGA
ACAGCTAGGGGCTCCTGAGATCTTTGAAAGAATATCTAATTACGAAAAAGGGCTAGTATTGGTCACAGGGCCGACTGGTT
CAGGTAAGTCGACAACTTTGGCAGCGATGGTGGATTACGTTAACCGTAACCATAATAAGCATATATTGACGATTGAAGAC
CCGATCGAATTTGTTCATACCAACAACAAATGCTTAATCAACCAACGAGAAGTGCATCGCGATACTCACAGCTTCAAAGC
GGCATTGCGTAGTGCGTTGCGTGAAGATCCAGACGTAATCCTTGTCGGCGAACTTCGTGACCAAGAGACGATCAGCTTAG
CGCTAACCGCAGCAGAAACGGGTCACTTAGTTTTTGGTACCTTGCATACTAGCTCTGCGGCAAAAACCATAGACCGTATT
ATCGATGTATTCCCGGGTAGCGACAAAGACATGGTGCGTTCAATGTTGTCTGAATCGTTACGTTCGGTGATTGCCCAGAA
GCTGTTAAAGCGTGTTGGTGGCGGTCGTGTGGCTTGTCATGAAATCATGATGGCGACACCTGCGATTAGAAACTTGATCC
GTGAAGACAAGGTCGCGCAGATGTATTCGATCATTCAAACGGGCGCGGCACACGGTATGCAGACCATGGAGCAAAATGCG
AAGCAGCTGATGGCACAAGGCTTAGTTGATTCAGAAGAGGTCGAGAAAAAGATCGAAATTGAAACCTCAATGTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1R3EAA6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

88.696

100

0.887
  pilT Vibrio cholerae strain A1552

88.696

100

0.887
  pilT Acinetobacter baumannii D1279779

72.59

96.232

0.699
  pilT Acinetobacter baumannii strain A118

72.59

96.232

0.699
  pilT Acinetobacter nosocomialis M2

72.289

96.232

0.696
  pilT Pseudomonas aeruginosa PAK

69.412

98.551

0.684
  pilT Acinetobacter baylyi ADP1

71.515

95.652

0.684
  pilT Pseudomonas stutzeri DSM 10701

69.139

97.681

0.675
  pilT Legionella pneumophila strain Lp02

65.507

100

0.655
  pilT Legionella pneumophila strain ERS1305867

65.507

100

0.655
  pilT Neisseria meningitidis 8013

62.609

100

0.626
  pilT Neisseria gonorrhoeae MS11

62.319

100

0.623
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.147

98.841

0.496
  pilU Vibrio cholerae strain A1552

42.769

94.203

0.403
  pilU Pseudomonas stutzeri DSM 10701

40.12

96.812

0.388
  pilU Acinetobacter baylyi ADP1

39.688

92.754

0.368