Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   LTQ02_RS11440 Genome accession   NZ_CP089205
Coordinates   2558098..2559207 (-) Length   369 a.a.
NCBI ID   WP_004735479.1    Uniprot ID   A0A1R3EZW5
Organism   Vibrio splendidus strain 4_C08b     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2553098..2564207
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LTQ02_RS11410 (LTQ02_11410) - 2553367..2554713 (-) 1347 WP_017095265.1 hypothetical protein -
  LTQ02_RS11415 (LTQ02_11415) rsmE 2554908..2555639 (+) 732 WP_017077778.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  LTQ02_RS11420 (LTQ02_11420) gshB 2555649..2556599 (+) 951 WP_004735482.1 glutathione synthase -
  LTQ02_RS11425 (LTQ02_11425) - 2556716..2557279 (+) 564 WP_017095267.1 YqgE/AlgH family protein -
  LTQ02_RS11430 (LTQ02_11430) ruvX 2557357..2557779 (+) 423 WP_004735480.1 Holliday junction resolvase RuvX -
  LTQ02_RS11435 (LTQ02_11435) - 2557878..2558039 (+) 162 WP_243578769.1 hypothetical protein -
  LTQ02_RS11440 (LTQ02_11440) pilU 2558098..2559207 (-) 1110 WP_004735479.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LTQ02_RS11445 (LTQ02_11445) pilT 2559222..2560259 (-) 1038 WP_004735478.1 type IV pilus twitching motility protein PilT Machinery gene
  LTQ02_RS11450 (LTQ02_11450) - 2560286..2560990 (+) 705 WP_243578768.1 YggS family pyridoxal phosphate-dependent enzyme -
  LTQ02_RS11455 (LTQ02_11455) proC 2561075..2561893 (+) 819 WP_108167423.1 pyrroline-5-carboxylate reductase -
  LTQ02_RS11460 (LTQ02_11460) - 2561969..2562526 (+) 558 WP_004735475.1 YggT family protein -
  LTQ02_RS11465 (LTQ02_11465) yggU 2562556..2562846 (+) 291 WP_243578767.1 DUF167 family protein YggU -
  LTQ02_RS11470 (LTQ02_11470) - 2562875..2563306 (+) 432 WP_004735472.1 DUF4426 domain-containing protein -
  LTQ02_RS11475 (LTQ02_11475) - 2563356..2563957 (+) 602 Protein_2241 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41351.25 Da        Isoelectric Point: 5.8700

>NTDB_id=635754 LTQ02_RS11440 WP_004735479.1 2558098..2559207(-) (pilU) [Vibrio splendidus strain 4_C08b]
MELNQILEGMLSQKASDLYITVDAPILFRVDGELRPQGEKLNAAQVAQLLDAMMDQDRRDEYQQTREANFAIVRDFGRFR
VSAFFQRELPGAVIRRIETNIPTFEQLKLPDVLQDLSIAKRGLVLVVGATGSGKSTSMAAMTGYRNTNRSGHILTVEDPI
EFVHEHKKCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKEQKEQFLFDLSMNLRGVVAQQLIRDKNGSGRHGVFEILLNSPRVSDLIRRGELHELKATMAKSKEIGMQTFDQALY
DLVLAGKISEEDAFHSADSANDLRLMLKTRRGDDDYGTGALAGVKIDMG

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=635754 LTQ02_RS11440 WP_004735479.1 2558098..2559207(-) (pilU) [Vibrio splendidus strain 4_C08b]
ATGGAATTGAATCAAATCCTTGAAGGGATGCTATCTCAAAAAGCATCGGATCTTTATATCACCGTTGATGCGCCTATCCT
GTTTCGTGTCGATGGTGAACTGCGACCTCAAGGCGAGAAATTGAATGCGGCTCAGGTTGCTCAGTTACTTGATGCGATGA
TGGATCAAGACCGACGCGATGAATATCAGCAAACGCGAGAGGCTAATTTTGCCATCGTACGTGATTTTGGTCGTTTTCGT
GTGAGTGCGTTTTTTCAGCGAGAGCTACCAGGAGCGGTCATTCGTCGTATCGAAACTAACATCCCAACCTTTGAGCAATT
AAAGCTTCCTGATGTACTGCAAGACCTGTCAATCGCAAAGCGTGGATTGGTGCTGGTGGTTGGTGCTACGGGCTCGGGTA
AATCAACCTCAATGGCTGCGATGACGGGCTATCGTAATACCAATCGTTCAGGGCATATTTTGACGGTTGAAGACCCAATT
GAATTTGTTCACGAACATAAAAAATGTATCGTAACTCAACGTGAGGTTGGGCTAGACACCGAGAGCTATGAAGTTGCGCT
GAAGAACTCTTTACGCCAAGCACCTGATATGATCTTGATTGGCGAAATCCGTAGCCGTGAAACCATGGAATACGCGATGA
CCTTTGCTGAAACGGGCCACTTGTGTATGGCAACACTGCATGCGAATAATGCCAACCAAGCGTTAGAACGTATTCTTCAC
TTGGTGCCGAAAGAACAGAAAGAGCAGTTCTTGTTTGATCTGTCGATGAACCTGCGTGGTGTGGTCGCTCAGCAATTAAT
CCGAGATAAGAATGGCAGCGGTCGCCATGGCGTGTTTGAGATTCTACTTAACAGCCCGCGAGTGTCTGACTTGATTCGTC
GTGGTGAGTTGCATGAACTAAAAGCGACTATGGCTAAATCGAAAGAGATTGGTATGCAGACCTTTGACCAAGCGTTGTAT
GACTTGGTGCTTGCAGGCAAGATCAGTGAAGAAGACGCATTCCACAGTGCCGATTCCGCGAATGACTTACGCTTAATGCT
AAAAACCAGACGTGGTGATGATGACTACGGAACCGGTGCTTTGGCTGGCGTGAAGATTGATATGGGTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(6-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1R3EZW5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

84.511

99.729

0.843
  pilU Pseudomonas stutzeri DSM 10701

56.065

100

0.564
  pilU Acinetobacter baylyi ADP1

54.416

95.122

0.518
  pilT Acinetobacter baumannii strain A118

41.813

92.683

0.388
  pilT Acinetobacter baumannii D1279779

41.813

92.683

0.388
  pilT Acinetobacter nosocomialis M2

41.813

92.683

0.388
  pilT Legionella pneumophila strain ERS1305867

42.183

91.87

0.388
  pilT Legionella pneumophila strain Lp02

42.183

91.87

0.388
  pilT Acinetobacter baylyi ADP1

42.169

89.973

0.379
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.21

87.805

0.379
  pilT Pseudomonas aeruginosa PAK

41.791

90.786

0.379
  pilT Pseudomonas stutzeri DSM 10701

40.299

90.786

0.366
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.419

90.515

0.366
  pilT Vibrio cholerae strain A1552

40.419

90.515

0.366
  pilT Neisseria gonorrhoeae MS11

38.841

93.496

0.363
  pilT Neisseria meningitidis 8013

38.841

93.496

0.363