Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LSO60_RS03610 Genome accession   NZ_CP089051
Coordinates   721639..722676 (-) Length   345 a.a.
NCBI ID   WP_262437553.1    Uniprot ID   A0AA46S5D6
Organism   Acinetobacter ursingii strain RIVM_C010559     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 716639..727676
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LSO60_RS03580 (LSO60_03580) - 717411..717860 (+) 450 WP_004991008.1 bacteriohemerythrin -
  LSO60_RS03585 (LSO60_03585) - 718023..719108 (+) 1086 WP_263512889.1 hypothetical protein -
  LSO60_RS03590 (LSO60_03590) - 719105..719425 (+) 321 WP_005002835.1 RnfH family protein -
  LSO60_RS03595 (LSO60_03595) - 719469..719867 (-) 399 WP_004991001.1 outer membrane protein assembly factor BamE -
  LSO60_RS03600 (LSO60_03600) fur 719979..720419 (+) 441 WP_004990998.1 ferric iron uptake transcriptional regulator -
  LSO60_RS03605 (LSO60_03605) pilU 720493..721611 (-) 1119 WP_004990996.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LSO60_RS03610 (LSO60_03610) pilT 721639..722676 (-) 1038 WP_262437553.1 type IV pilus twitching motility protein PilT Machinery gene
  LSO60_RS03615 (LSO60_03615) - 722800..723486 (+) 687 WP_263512890.1 YggS family pyridoxal phosphate-dependent enzyme -
  LSO60_RS03620 (LSO60_03620) - 723637..724482 (+) 846 WP_263512891.1 undecaprenyl-diphosphate phosphatase -
  LSO60_RS03625 (LSO60_03625) - 724440..725132 (-) 693 WP_263512892.1 HAD family hydrolase -
  LSO60_RS03630 (LSO60_03630) - 725213..725998 (-) 786 WP_263512893.1 transglutaminase family protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38374.01 Da        Isoelectric Point: 6.5052

>NTDB_id=634810 LSO60_RS03610 WP_262437553.1 721639..722676(-) (pilT) [Acinetobacter ursingii strain RIVM_C010559]
MDITELLAFSVKNGASDLHLSAGMPPMIRVDGEVRRINLPALEHKEVHRLVYDIMNDKQRRDFEEQLETDFSFEVPNIAR
FRVNAFNQNRGAGAVFRTIPSKVLTMDDLGLGQIFKDICDYPRGIVLVTGPTGSGKSTTLAAMLDYINDNRYDHILTVED
PIEFVHQSKKCLINQREVHRDTHGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAEEKDMVRAMLSESLQAVISQTLLKKNGGGRVAAHEIMIGIPAIRNLIRENKVAQMYSAIQTGANYGMTTLDQSL
KTLVSKGMISPQTARTVAKSPESFL

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=634810 LSO60_RS03610 WP_262437553.1 721639..722676(-) (pilT) [Acinetobacter ursingii strain RIVM_C010559]
ATGGATATCACAGAATTACTCGCCTTTTCGGTTAAAAACGGTGCATCAGATTTACATCTTTCCGCAGGAATGCCACCGAT
GATTCGTGTCGATGGTGAGGTTCGTCGTATTAATCTACCTGCACTGGAACACAAAGAAGTTCATCGCCTTGTTTATGACA
TTATGAATGATAAACAACGTCGTGACTTTGAAGAACAACTTGAAACTGACTTTTCCTTTGAAGTTCCAAACATTGCGCGT
TTTCGTGTCAATGCCTTTAACCAAAATCGTGGTGCAGGTGCAGTGTTTCGTACCATTCCGTCTAAAGTTCTCACCATGGA
TGATTTGGGTTTAGGACAAATCTTTAAGGATATCTGTGATTATCCACGTGGTATTGTACTGGTTACAGGCCCAACGGGTT
CGGGTAAATCAACCACGCTTGCCGCCATGCTAGATTATATCAATGACAATCGTTATGACCATATTTTAACCGTTGAAGAT
CCGATCGAATTTGTACATCAATCCAAAAAGTGCTTGATTAACCAACGTGAAGTACATCGCGATACTCATGGTTTTAATGA
AGCACTACGTTCAGCATTACGTGAAGACCCTGATATCATCCTTGTCGGTGAGATGCGCGACCTTGAAACCATTCGTTTGG
CTTTGACCGCCGCAGAAACGGGTCACTTGGTATTTGGTACATTGCATACCACATCTGCTGCAAAAACCATTGACCGTGTA
ATTGACGTATTCCCTGCTGAAGAAAAAGATATGGTTCGTGCCATGTTATCTGAATCTTTACAAGCCGTTATTTCTCAAAC
ATTATTGAAGAAAAATGGGGGTGGCCGTGTCGCAGCGCATGAGATTATGATCGGGATACCTGCGATCCGTAACTTAATCC
GTGAAAATAAAGTCGCGCAAATGTATTCTGCTATTCAGACAGGTGCCAACTATGGCATGACCACACTAGATCAAAGCCTC
AAAACATTGGTTTCAAAAGGCATGATCAGTCCACAAACTGCGCGAACAGTTGCCAAGTCACCCGAATCCTTCCTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

96.522

100

0.965
  pilT Acinetobacter baumannii D1279779

96.232

100

0.962
  pilT Acinetobacter baumannii strain A118

96.232

100

0.962
  pilT Acinetobacter baylyi ADP1

88.663

99.71

0.884
  pilT Pseudomonas aeruginosa PAK

83.721

99.71

0.835
  pilT Pseudomonas stutzeri DSM 10701

83.14

99.71

0.829
  pilT Legionella pneumophila strain Lp02

74.709

99.71

0.745
  pilT Legionella pneumophila strain ERS1305867

74.709

99.71

0.745
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.048

97.391

0.672
  pilT Vibrio cholerae strain A1552

69.048

97.391

0.672
  pilT Neisseria meningitidis 8013

67.246

100

0.672
  pilT Neisseria gonorrhoeae MS11

66.957

100

0.67
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.304

100

0.513
  pilU Vibrio cholerae strain A1552

41.349

98.841

0.409
  pilU Pseudomonas stutzeri DSM 10701

40.762

98.841

0.403
  pilU Acinetobacter baylyi ADP1

39.595

100

0.397