Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   LSO60_RS03605 Genome accession   NZ_CP089051
Coordinates   720493..721611 (-) Length   372 a.a.
NCBI ID   WP_004990996.1    Uniprot ID   A0A3F3L4S7
Organism   Acinetobacter ursingii strain RIVM_C010559     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 715493..726611
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LSO60_RS03575 (LSO60_03575) - 716227..717075 (+) 849 WP_263512888.1 class II glutamine amidotransferase -
  LSO60_RS03580 (LSO60_03580) - 717411..717860 (+) 450 WP_004991008.1 bacteriohemerythrin -
  LSO60_RS03585 (LSO60_03585) - 718023..719108 (+) 1086 WP_263512889.1 hypothetical protein -
  LSO60_RS03590 (LSO60_03590) - 719105..719425 (+) 321 WP_005002835.1 RnfH family protein -
  LSO60_RS03595 (LSO60_03595) - 719469..719867 (-) 399 WP_004991001.1 outer membrane protein assembly factor BamE -
  LSO60_RS03600 (LSO60_03600) fur 719979..720419 (+) 441 WP_004990998.1 ferric iron uptake transcriptional regulator -
  LSO60_RS03605 (LSO60_03605) pilU 720493..721611 (-) 1119 WP_004990996.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LSO60_RS03610 (LSO60_03610) pilT 721639..722676 (-) 1038 WP_262437553.1 type IV pilus twitching motility protein PilT Machinery gene
  LSO60_RS03615 (LSO60_03615) - 722800..723486 (+) 687 WP_263512890.1 YggS family pyridoxal phosphate-dependent enzyme -
  LSO60_RS03620 (LSO60_03620) - 723637..724482 (+) 846 WP_263512891.1 undecaprenyl-diphosphate phosphatase -
  LSO60_RS03625 (LSO60_03625) - 724440..725132 (-) 693 WP_263512892.1 HAD family hydrolase -
  LSO60_RS03630 (LSO60_03630) - 725213..725998 (-) 786 WP_263512893.1 transglutaminase family protein -

Sequence

Protein


Download         Length: 372 a.a.        Molecular weight: 41822.00 Da        Isoelectric Point: 6.0604

>NTDB_id=634809 LSO60_RS03605 WP_004990996.1 720493..721611(-) (pilU) [Acinetobacter ursingii strain RIVM_C010559]
MDFNDLLNLMIEQQASDLFVTAGVEPSMKINGQIVPVSKTKLNAEVVGQLIQSIMSDKQRKEFDESRECNFAIVNREKTA
RFRVSAFQQRDMPGMVLRRIETIIPTMDELRLPPILKDLAMTKRGIIIFVGATGTGKSTSLASMIGYRNQNSKGHIITVE
DPIEFIHEHAGCIVTQREVGIDTDSFDVALKNTLRQAPDVILIGEIRSREVMDYAIAFAETGHLVLATLHANNANQALDR
IIHFFESDRHNQLYMDLSLNLKAMVAQQLIPTPDGNSRRAAIEILINTPLLADYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLSIKLSEEGAEQLLNASRNITFDGQ

Nucleotide


Download         Length: 1119 bp        

>NTDB_id=634809 LSO60_RS03605 WP_004990996.1 720493..721611(-) (pilU) [Acinetobacter ursingii strain RIVM_C010559]
ATGGATTTTAATGATCTTCTCAATCTGATGATTGAACAACAAGCATCAGACCTGTTTGTCACCGCAGGTGTGGAACCTTC
GATGAAAATTAATGGTCAGATTGTCCCAGTGTCTAAGACTAAGCTGAATGCTGAGGTCGTCGGACAACTGATTCAGTCCA
TTATGTCTGATAAGCAACGCAAGGAATTTGACGAGAGCAGAGAGTGTAACTTTGCGATTGTAAATCGGGAAAAAACCGCA
CGTTTTCGTGTCAGTGCATTTCAGCAGCGTGATATGCCAGGCATGGTATTACGTCGTATCGAAACGATTATCCCGACAAT
GGATGAATTACGCTTACCGCCCATTCTTAAAGATCTGGCCATGACCAAACGTGGCATTATTATCTTTGTTGGGGCAACCG
GTACGGGTAAATCCACTTCATTGGCATCCATGATTGGTTATCGAAATCAGAACTCCAAAGGTCATATCATCACGGTTGAA
GATCCAATCGAGTTCATTCATGAACACGCTGGTTGTATTGTGACTCAACGTGAAGTCGGTATTGATACAGACTCTTTCGA
TGTAGCTCTGAAAAACACACTACGACAAGCCCCTGATGTGATTTTGATTGGTGAGATTCGTTCACGTGAGGTCATGGACT
ACGCCATTGCCTTTGCTGAAACTGGACATCTGGTCTTAGCGACATTACACGCCAACAACGCCAACCAAGCATTGGATCGT
ATTATTCATTTCTTTGAAAGTGATCGCCACAATCAGCTCTATATGGATCTCTCGCTCAATCTAAAAGCAATGGTGGCACA
GCAATTGATTCCAACACCAGATGGCAATTCACGTCGTGCTGCGATTGAAATTCTGATCAATACACCATTACTTGCTGACT
ATATTCGTAAAGGTGAAATTCATGAAATCAAAGATTTAATGAAACGTTCACGTGAACTCGGCATGCAAACCTTTGATCAG
GCTTTATTTGATTTATATAAAGCAGGTCAAATTACCTATAAAGATGCCTTAAAGCATGCCGACTCACCAAACGATCTACG
CCTCAGTATCAAACTTTCAGAGGAAGGTGCAGAACAACTCTTAAATGCCAGCCGTAATATTACTTTCGATGGTCAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3F3L4S7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

76.984

100

0.782
  pilU Pseudomonas stutzeri DSM 10701

69.811

99.731

0.696
  pilU Vibrio cholerae strain A1552

55.84

94.355

0.527
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.484

97.849

0.406
  pilT Legionella pneumophila strain Lp02

40.828

90.86

0.371
  pilT Legionella pneumophila strain ERS1305867

40.828

90.86

0.371
  pilT Pseudomonas aeruginosa PAK

39.306

93.011

0.366
  pilT Acinetobacter baumannii strain A118

39.067

92.204

0.36
  pilT Acinetobacter baumannii D1279779

39.067

92.204

0.36
  pilT Acinetobacter baylyi ADP1

39.067

92.204

0.36
  pilT Acinetobacter nosocomialis M2

39.067

92.204

0.36