Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LRO84_RS14200 Genome accession   NZ_CP088955
Coordinates   3017140..3018177 (+) Length   345 a.a.
NCBI ID   WP_003653239.1    Uniprot ID   A0A0B2UAY6
Organism   Acinetobacter calcoaceticus strain ARu19     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3012140..3023177
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LRO84_RS14190 (LRO84_14190) - 3012524..3016120 (+) 3597 WP_231761771.1 AAA family ATPase -
  LRO84_RS14195 (LRO84_14195) - 3016321..3017013 (-) 693 WP_231761772.1 YggS family pyridoxal phosphate-dependent enzyme -
  LRO84_RS14200 (LRO84_14200) pilT 3017140..3018177 (+) 1038 WP_003653239.1 type IV pilus twitching motility protein PilT Machinery gene
  LRO84_RS14205 (LRO84_14205) pilU 3018197..3019315 (+) 1119 WP_004641663.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LRO84_RS14210 (LRO84_14210) fur 3019385..3019822 (-) 438 WP_003653242.1 ferric iron uptake transcriptional regulator -
  LRO84_RS14215 (LRO84_14215) - 3019934..3020332 (+) 399 WP_002121740.1 outer membrane protein assembly factor BamE -
  LRO84_RS14220 (LRO84_14220) - 3020400..3020711 (-) 312 WP_005048580.1 RnfH family protein -
  LRO84_RS14225 (LRO84_14225) - 3020711..3021778 (-) 1068 WP_198077856.1 hypothetical protein -
  LRO84_RS14230 (LRO84_14230) - 3021930..3022385 (-) 456 WP_017388663.1 bacteriohemerythrin -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38348.92 Da        Isoelectric Point: 6.7269

>NTDB_id=634212 LRO84_RS14200 WP_003653239.1 3017140..3018177(+) (pilT) [Acinetobacter calcoaceticus strain ARu19]
MDITELLAFSVKNGASDLHLSAGMPPMIRVDGEVRRINLPALDHKDVHRLVYDIMNDKQRRDYEEKLETDFSFEVPNVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGLGQIFKDICDYPRGIVLVTGPTGSGKSTTLAAMMDYINENRYDHILTVED
PIEFVHQSKKCLINQREVHRDTHGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAEEKDMVRAMLSESLQAVISQTLLKKNGGGRVAAHEIMIGIPAIRNLIRENKVAQMYSSIQTGANHGMTTLDQSL
KGLVSRGVISPQTARTAAKQPESFL

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=634212 LRO84_RS14200 WP_003653239.1 3017140..3018177(+) (pilT) [Acinetobacter calcoaceticus strain ARu19]
ATGGATATTACAGAGCTACTCGCTTTCTCTGTGAAAAATGGCGCGTCCGATTTACACTTATCTGCTGGCATGCCACCAAT
GATTCGCGTGGATGGTGAAGTCCGCCGCATTAACTTACCAGCTTTAGACCACAAAGATGTTCACCGTCTGGTCTATGACA
TCATGAATGATAAACAACGCCGTGACTATGAAGAAAAGCTCGAAACAGACTTTTCTTTTGAAGTTCCCAATGTTGCGCGT
TTTCGTGTCAATGCATTTAACCAGAACCGCGGTGCGGGCGCAGTGTTTCGTACCATTCCATCAAAAGTACTCACCATGGA
GGATTTAGGCTTAGGGCAAATCTTTAAAGATATTTGTGATTACCCACGTGGCATTGTGTTAGTTACAGGACCAACAGGTT
CGGGGAAATCAACCACGCTTGCAGCGATGATGGACTATATCAACGAAAATCGTTATGACCATATTTTAACTGTTGAAGAC
CCAATCGAATTTGTACACCAGTCGAAGAAATGTTTGATTAACCAGCGTGAAGTGCATCGTGACACACATGGCTTTAATGA
AGCACTACGTTCGGCACTGCGTGAAGACCCCGATATTATTTTGGTCGGTGAGATGCGTGACCTTGAAACCATTCGTTTAG
CACTTACCGCTGCTGAAACTGGTCACTTGGTATTTGGTACACTCCATACGACTTCAGCTGCAAAAACCATTGACCGTGTG
ATTGACGTATTCCCTGCCGAAGAAAAAGACATGGTTCGTGCCATGTTGTCAGAATCTTTACAGGCTGTTATTTCGCAAAC
CCTACTTAAAAAGAACGGCGGTGGGCGTGTCGCAGCACATGAAATCATGATTGGTATTCCTGCCATTCGTAACTTAATTC
GTGAAAATAAAGTCGCACAAATGTATTCATCAATTCAGACTGGTGCAAATCATGGCATGACGACACTCGACCAAAGCTTA
AAAGGTCTAGTTTCTCGTGGTGTGATTAGTCCACAAACTGCACGTACTGCTGCAAAACAGCCTGAATCATTCCTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0B2UAY6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

99.13

100

0.991
  pilT Acinetobacter baumannii D1279779

98.841

100

0.988
  pilT Acinetobacter baumannii strain A118

98.841

100

0.988
  pilT Acinetobacter baylyi ADP1

88.953

99.71

0.887
  pilT Pseudomonas stutzeri DSM 10701

84.012

99.71

0.838
  pilT Pseudomonas aeruginosa PAK

83.14

99.71

0.829
  pilT Legionella pneumophila strain Lp02

75.581

99.71

0.754
  pilT Legionella pneumophila strain ERS1305867

75.581

99.71

0.754
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.429

97.391

0.696
  pilT Vibrio cholerae strain A1552

71.429

97.391

0.696
  pilT Neisseria meningitidis 8013

68.116

100

0.681
  pilT Neisseria gonorrhoeae MS11

67.826

100

0.678
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.174

100

0.522
  pilU Vibrio cholerae strain A1552

41.593

98.261

0.409
  pilU Pseudomonas stutzeri DSM 10701

40.597

97.101

0.394
  pilU Acinetobacter baylyi ADP1

39.017

100

0.391