Detailed information NTG0634213

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: LRO84_RS14205
• Genome accession: NZ_CP088955
• Coordinates: 3018197..3019315 (+)
• Length: 372 a.a.
• NCBI ID: WP_004641663.1
• Uniprot ID: A0A0B2UBU9
• Organism: Acinetobacter calcoaceticus strain ARu19
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3013197..3024315

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LRO84_RS14195 (LRO84_14195)	-	3016321..3017013 (-)	693	WP_231761772.1	YggS family pyridoxal phosphate-dependent enzyme	-
LRO84_RS14200 (LRO84_14200)	pilT	3017140..3018177 (+)	1038	WP_003653239.1	type IV pilus twitching motility protein PilT	Machinery gene
LRO84_RS14205 (LRO84_14205)	pilU	3018197..3019315 (+)	1119	WP_004641663.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
LRO84_RS14210 (LRO84_14210)	fur	3019385..3019822 (-)	438	WP_003653242.1	ferric iron uptake transcriptional regulator	-
LRO84_RS14215 (LRO84_14215)	-	3019934..3020332 (+)	399	WP_002121740.1	outer membrane protein assembly factor BamE	-
LRO84_RS14220 (LRO84_14220)	-	3020400..3020711 (-)	312	WP_005048580.1	RnfH family protein	-
LRO84_RS14225 (LRO84_14225)	-	3020711..3021778 (-)	1068	WP_198077856.1	hypothetical protein	-
LRO84_RS14230 (LRO84_14230)	-	3021930..3022385 (-)	456	WP_017388663.1	bacteriohemerythrin	-
LRO84_RS14235 (LRO84_14235)	-	3022637..3023485 (-)	849	WP_231761773.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41873.07 Da
• Isoelectric Point: 6.6400

>NTDB_id=634213 LRO84_RS14205 WP_004641663.1 3018197..3019315(+) (pilU) [Acinetobacter calcoaceticus strain ARu19]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGDVIGQLLHSIMSEKQRKEFADTRECNFAIMNRDKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISYRNHNTKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLAEEGPDQIAGTNQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=634213 LRO84_RS14205 WP_004641663.1 3018197..3019315(+) (pilU) [Acinetobacter calcoaceticus strain ARu19]
ATGGATTTTAATGACCTACTCAACCTCATGGTTGAAAAAAAATCATCCGATCTTTTTATTACAGATGGTGTTGCACCATC
TATGAAAATTAACGGGCAAATTGTTCCAATTTCAAAAAATAGTCTATCTGGGGACGTTATTGGTCAACTTTTACATTCCA
TCATGAGCGAAAAACAACGCAAAGAATTTGCAGACACTCGTGAATGTAACTTTGCCATTATGAACCGTGATAAAACGGCC
CGCTTTCGTGTGAGCGCTTTTCAGCAGCGTGACATGCCTGGCATGGTGCTACGCCGAATTGAAACTAAAATTCCTTCCAT
TGATGACTTGCAGTTACCGCCCGTTCTTAAAGATTTATCAATGACCAAACGCGGCATCATTATTTTTGTTGGCGCAACAG
GTACTGGTAAATCGACTTCATTGGCTTCAATGATCAGCTATCGCAATCACAATACTAAAGGTCATATCATTACTATTGAA
GACCCAATCGAGTTTATTCATGAACATGCAGGCTGCATCATTACTCAACGTGAAGTCGGGATCGATACAGACTCATTTGA
AATTGCCTTAAAGAATACTTTGCGACAAGCACCAGATGTCATTTTAATTGGTGAGATTCGCTCTCGTGAAGTTATGGACT
ACGCGATTGGCTTTGCTGAAACAGGTCACCTTGTATTGGCTACGATGCACGCTAACAACGCCAACCAAGCGCTCGATCGT
ATTATTCACTTCTTTGAAAGTGACCGCCACAGTCAGCTTTACATGGACTTATCGCTTAACTTAAAGGCCATGATTGCACA
GCAACTTATTCCAACACCAGATGGCAATTCACGTCGTGCAGCCATTGAGATTTTGATTAACTCGCCATTAATTTCTGACT
ACATTCGTAAAGGCGAAATTCATGAAATTAAAGATTTAATGAAGCGTTCGCGTGAACTGGGTATGCAAACCTTTGACCAA
GCGTTGTTTGATCTCTATAAAGCAGGCCAAATTACCTATAAAGATGCATTAAAACATGCCGACTCACCGAATGATTTGCG
TTTGACCATTAAGCTTGCCGAAGAAGGACCTGATCAAATTGCAGGCACAAATCAGCATTTAACCTTCGACCGACAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (32-270)

3D structure

• AlphaFold DB: A0A0B2UBU9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.132	100	0.763
pilU	Pseudomonas stutzeri DSM 10701	67.655	99.731	0.675
pilU	Vibrio cholerae strain A1552	54.054	99.462	0.538
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	40.659	97.849	0.398
pilT	Legionella pneumophila strain Lp02	39.645	90.86	0.36
pilT	Legionella pneumophila strain ERS1305867	39.645	90.86	0.36