Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LSQ66_RS01235 Genome accession   NZ_CP088952
Coordinates   259423..260466 (-) Length   347 a.a.
NCBI ID   WP_231768006.1    Uniprot ID   -
Organism   Massilia endophytica strain DM-R-R2A-13     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 254423..265466
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LSQ66_RS24790 - 254731..255120 (+) 390 WP_407659617.1 lysozyme inhibitor LprI family protein -
  LSQ66_RS01220 (LSQ66_01220) - 255172..257670 (-) 2499 WP_231768003.1 EAL domain-containing protein -
  LSQ66_RS01225 (LSQ66_01225) - 257792..258277 (-) 486 WP_231768004.1 glutathione peroxidase -
  LSQ66_RS01230 (LSQ66_01230) pilU 258277..259413 (-) 1137 WP_231768005.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LSQ66_RS01235 (LSQ66_01235) pilT 259423..260466 (-) 1044 WP_231768006.1 type IV pilus twitching motility protein PilT Machinery gene
  LSQ66_RS01240 (LSQ66_01240) - 260521..261228 (+) 708 WP_231768007.1 YggS family pyridoxal phosphate-dependent enzyme -
  LSQ66_RS01245 (LSQ66_01245) proC 261231..262079 (+) 849 WP_231768008.1 pyrroline-5-carboxylate reductase -
  LSQ66_RS01250 (LSQ66_01250) - 262194..262559 (-) 366 WP_231768009.1 YqjD family protein -
  LSQ66_RS01255 (LSQ66_01255) ubiA 262598..263473 (-) 876 WP_231768010.1 4-hydroxybenzoate octaprenyltransferase -
  LSQ66_RS01260 (LSQ66_01260) - 263507..264448 (-) 942 WP_231768011.1 hydrogen peroxide-inducible genes activator -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38079.77 Da        Isoelectric Point: 6.7269

>NTDB_id=634096 LSQ66_RS01235 WP_231768006.1 259423..260466(-) (pilT) [Massilia endophytica strain DM-R-R2A-13]
MDISELLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINLPPLEHKDVHGMIYDIMNDGQRKAYEEMLECDFSFAIPGLAR
FRVNAYNQERGASAVMRTIPSKILTLEELNAPKIFAEFALKPRGLVLVTGPTGSGKSTTLAAMINHLNENEYGHILTIED
PIEFVHESKKCLINQREVGPHTLSFNNALRSALREDPDAILVGELRDLETIRLALSAAETGHLVFGTLHTSSAAKTIDRI
VDVFPAEEKEMVRAMLSESLQAVISQTLLKTKDGSGRVAAHEIMVGTPAIRNLIREAKIAQMYSAIQTGSNVGMQTLDQN
LTDLVRRNVISSAAARNAAKIPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=634096 LSQ66_RS01235 WP_231768006.1 259423..260466(-) (pilT) [Massilia endophytica strain DM-R-R2A-13]
ATGGACATCTCCGAACTACTCGCATTCTCCGTCAAGAACAAGGCATCCGACCTCCACCTCTCGGCCGGCCTGCCGCCCAT
GATCCGCGTGCACGGCGACGTCCGCCGCATCAACCTGCCACCGCTTGAGCACAAGGACGTGCACGGCATGATCTATGACA
TCATGAACGACGGCCAGCGCAAGGCCTACGAGGAAATGCTGGAGTGCGACTTCTCCTTCGCCATTCCCGGCCTGGCGCGC
TTCCGTGTGAACGCCTACAACCAGGAGCGCGGCGCCTCGGCGGTGATGCGGACCATTCCGTCCAAGATCCTGACGCTGGA
AGAGCTGAACGCACCGAAGATCTTCGCCGAATTCGCCCTCAAGCCGCGCGGCCTGGTGCTCGTTACCGGCCCCACCGGCT
CGGGCAAATCGACCACGCTGGCGGCCATGATCAACCACCTGAACGAGAACGAGTACGGCCACATCCTCACCATCGAGGAC
CCGATCGAGTTCGTGCACGAATCGAAGAAGTGCCTGATCAACCAGCGGGAAGTCGGCCCGCACACCCTCTCCTTCAACAA
CGCGCTGCGCTCCGCGCTGCGTGAAGACCCGGACGCCATCCTGGTCGGCGAGCTGCGCGACCTGGAAACCATCCGCCTCG
CCTTGTCCGCCGCCGAAACGGGCCACCTGGTGTTCGGCACCCTGCACACCTCCTCCGCCGCCAAGACCATCGACCGTATC
GTCGACGTGTTCCCGGCCGAGGAAAAGGAGATGGTGCGCGCCATGCTGTCCGAATCGCTGCAGGCCGTCATCTCGCAAAC
GCTGCTGAAGACGAAGGACGGTTCCGGCCGCGTGGCCGCCCACGAAATCATGGTGGGCACGCCCGCGATCCGCAACCTGA
TCCGCGAGGCGAAGATCGCGCAGATGTATTCCGCCATCCAGACCGGCAGCAATGTCGGCATGCAGACGCTGGACCAGAAC
CTCACGGACCTCGTGCGCCGCAACGTGATCTCGTCCGCCGCCGCCCGCAACGCGGCCAAGATCCCCGAAAACTTCCCTGG
CTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

77.391

99.424

0.769
  pilT Acinetobacter baumannii D1279779

77.391

99.424

0.769
  pilT Acinetobacter baumannii strain A118

77.391

99.424

0.769
  pilT Pseudomonas aeruginosa PAK

77.391

99.424

0.769
  pilT Pseudomonas stutzeri DSM 10701

75.362

99.424

0.749
  pilT Acinetobacter baylyi ADP1

74.783

99.424

0.744
  pilT Legionella pneumophila strain Lp02

75.221

97.695

0.735
  pilT Legionella pneumophila strain ERS1305867

75.221

97.695

0.735
  pilT Neisseria gonorrhoeae MS11

68.986

99.424

0.686
  pilT Neisseria meningitidis 8013

68.986

99.424

0.686
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.277

95.677

0.663
  pilT Vibrio cholerae strain A1552

69.277

95.677

0.663
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.174

99.424

0.519
  pilU Acinetobacter baylyi ADP1

41.908

99.712

0.418
  pilU Vibrio cholerae strain A1552

41.298

97.695

0.403
  pilU Pseudomonas stutzeri DSM 10701

41.493

96.542

0.401