Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   LSQ66_RS01230 Genome accession   NZ_CP088952
Coordinates   258277..259413 (-) Length   378 a.a.
NCBI ID   WP_231768005.1    Uniprot ID   -
Organism   Massilia endophytica strain DM-R-R2A-13     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 253277..264413
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LSQ66_RS01210 (LSQ66_01210) - 253395..253919 (-) 525 WP_231768001.1 DUF4142 domain-containing protein -
  LSQ66_RS01215 (LSQ66_01215) - 253968..254435 (-) 468 WP_231768002.1 YaiI/YqxD family protein -
  LSQ66_RS24790 - 254731..255120 (+) 390 WP_407659617.1 lysozyme inhibitor LprI family protein -
  LSQ66_RS01220 (LSQ66_01220) - 255172..257670 (-) 2499 WP_231768003.1 EAL domain-containing protein -
  LSQ66_RS01225 (LSQ66_01225) - 257792..258277 (-) 486 WP_231768004.1 glutathione peroxidase -
  LSQ66_RS01230 (LSQ66_01230) pilU 258277..259413 (-) 1137 WP_231768005.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LSQ66_RS01235 (LSQ66_01235) pilT 259423..260466 (-) 1044 WP_231768006.1 type IV pilus twitching motility protein PilT Machinery gene
  LSQ66_RS01240 (LSQ66_01240) - 260521..261228 (+) 708 WP_231768007.1 YggS family pyridoxal phosphate-dependent enzyme -
  LSQ66_RS01245 (LSQ66_01245) proC 261231..262079 (+) 849 WP_231768008.1 pyrroline-5-carboxylate reductase -
  LSQ66_RS01250 (LSQ66_01250) - 262194..262559 (-) 366 WP_231768009.1 YqjD family protein -
  LSQ66_RS01255 (LSQ66_01255) ubiA 262598..263473 (-) 876 WP_231768010.1 4-hydroxybenzoate octaprenyltransferase -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41703.15 Da        Isoelectric Point: 6.4213

>NTDB_id=634095 LSQ66_RS01230 WP_231768005.1 258277..259413(-) (pilU) [Massilia endophytica strain DM-R-R2A-13]
MERDQASKFMFDLLRLMVSKKGSDLFITAGFPPAMKIDGKMTPVSQQALTPAHTADLARSIMNDKQTASFEATKEANFAI
APGDLGRFRVSAFVQMGSVGMVLRVINSSIPKLEDLGLPEVLKDVIMAKRGLVIMVGATGSGKSTTLAAMVGYRNANSYG
HIITIEDPVEFVHPHGNCVVTQREVGVDTDDWEIALKNTLRQAPDVIQIGEIRDRQTMDHAIAFAETGHLCLATLHANSS
NQALDRIINFFPEERRQQLLMDLSLNLKGMISQRLIPLKEAKGRAVAIEIMLNSPLIADLIFKGEVHEIKELMKKSRELG
MQTFDQSLFDLYEAGKISYEDALRNADSVNDLRLAIKLESKDARNRDLAAGTEHLGLV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=634095 LSQ66_RS01230 WP_231768005.1 258277..259413(-) (pilU) [Massilia endophytica strain DM-R-R2A-13]
ATGGAACGCGACCAGGCATCCAAATTCATGTTCGACCTGCTGCGCCTCATGGTCAGCAAAAAAGGCTCGGACCTTTTCAT
CACCGCCGGCTTCCCGCCCGCGATGAAAATCGACGGCAAGATGACGCCCGTATCGCAGCAGGCCCTCACGCCCGCGCACA
CGGCGGACCTGGCCCGTTCGATCATGAACGACAAGCAGACCGCCAGCTTCGAGGCAACGAAAGAAGCCAACTTCGCCATC
GCCCCCGGCGACCTGGGACGCTTCCGCGTTTCCGCCTTCGTGCAGATGGGTTCCGTGGGCATGGTGCTGCGCGTGATTAA
CAGCTCCATTCCGAAGCTGGAAGACCTGGGCCTGCCCGAGGTGCTGAAGGACGTGATCATGGCCAAGCGCGGCCTGGTTA
TCATGGTGGGCGCCACGGGCTCCGGCAAATCGACCACGCTGGCGGCCATGGTGGGCTACCGCAACGCCAACAGCTACGGC
CATATCATCACGATCGAGGACCCGGTGGAATTCGTTCACCCGCACGGCAACTGCGTGGTGACCCAGCGCGAAGTGGGCGT
GGACACGGATGACTGGGAAATCGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTCATCCAGATCGGCGAGATCCGCG
ACCGCCAGACCATGGACCACGCCATCGCCTTCGCCGAAACCGGCCACCTCTGCCTGGCCACGCTGCACGCGAACAGCTCC
AACCAGGCCCTGGACCGCATCATCAACTTCTTCCCCGAAGAGCGCCGCCAGCAGCTGCTGATGGACCTTTCCCTGAACCT
GAAAGGCATGATCTCCCAGCGCCTGATTCCGCTCAAGGAAGCCAAGGGCCGCGCGGTCGCCATCGAGATCATGCTGAACT
CCCCGCTGATCGCGGACCTGATCTTCAAGGGCGAAGTCCACGAGATCAAGGAGTTGATGAAGAAGTCGCGCGAACTGGGC
ATGCAGACCTTCGACCAGTCGCTTTTCGACCTCTACGAAGCGGGCAAGATCTCCTACGAAGACGCGCTGCGCAACGCGGA
CTCCGTCAACGACCTGCGCCTGGCCATCAAGCTCGAAAGCAAGGACGCGCGCAACCGCGACCTCGCGGCCGGCACCGAAC
ACCTGGGCCTCGTGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(53-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

64.756

92.328

0.598
  pilU Acinetobacter baylyi ADP1

59.77

92.063

0.55
  pilU Vibrio cholerae strain A1552

57.225

91.534

0.524
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.687

88.624

0.378
  pilT Pseudomonas aeruginosa PAK

42.687

88.624

0.378
  pilT Acinetobacter nosocomialis M2

42.296

87.566

0.37
  pilT Acinetobacter baylyi ADP1

42.296

87.566

0.37
  pilT Acinetobacter baumannii D1279779

42.296

87.566

0.37
  pilT Acinetobacter baumannii strain A118

42.296

87.566

0.37
  pilT Pseudomonas stutzeri DSM 10701

41.493

88.624

0.368