Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LRK32_RS04235 Genome accession   NZ_CP088920
Coordinates   949786..950823 (+) Length   345 a.a.
NCBI ID   WP_007507061.1    Uniprot ID   I4WZ44
Organism   Rhodanobacter denitrificans strain FW104-10F02     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 944786..955823
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LRK32_RS04210 (LRK32_04210) pdxH 945513..946100 (+) 588 WP_015446990.1 pyridoxamine 5'-phosphate oxidase -
  LRK32_RS04215 (LRK32_04215) - 946097..946537 (+) 441 WP_015446991.1 secondary thiamine-phosphate synthase enzyme YjbQ -
  LRK32_RS04220 (LRK32_04220) - 946542..947366 (+) 825 WP_037115662.1 cobalamin-binding protein -
  LRK32_RS04225 (LRK32_04225) - 948021..948596 (-) 576 WP_007507063.1 YggT family protein -
  LRK32_RS04230 (LRK32_04230) proC 948593..949417 (-) 825 WP_015446993.1 pyrroline-5-carboxylate reductase -
  LRK32_RS04235 (LRK32_04235) pilT 949786..950823 (+) 1038 WP_007507061.1 type IV pilus twitching motility protein PilT Machinery gene
  LRK32_RS04240 (LRK32_04240) pilU 950977..952143 (+) 1167 WP_015446994.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LRK32_RS04245 (LRK32_04245) - 952210..952587 (-) 378 WP_015446995.1 BON domain-containing protein -
  LRK32_RS04250 (LRK32_04250) - 952940..955150 (-) 2211 WP_231378422.1 TonB-dependent siderophore receptor -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38502.20 Da        Isoelectric Point: 6.8317

>NTDB_id=633944 LRK32_RS04235 WP_007507061.1 949786..950823(+) (pilT) [Rhodanobacter denitrificans strain FW104-10F02]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALEHKQVHSLIYDIMSDKQRRDYEEFYETDFSFEIPGLAR
FRVNAFNQNRGAGAVFRTIPSEVLTLEDLGCPRIFKELIEQPQGLILVTGPTGSGKSTTLAAMVDHINKNEYGHMLTIED
PIEFVHTSQKCLVNQREIHRDTLGFNEALRSALREDPDYILVGELRDLETIRLALTAAETGHLVFATVHTSSAAKTIDRI
IDVFPAGEKPMVRSMLSESLRAVISQSLLKKVGGGRIAAHEIMVGIPAIRNLIREDKVAQMYSSIQTGQQFGMQTLDQCL
QDLVKRGLVTRQQASAYAKNKDSFK

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=633944 LRK32_RS04235 WP_007507061.1 949786..950823(+) (pilT) [Rhodanobacter denitrificans strain FW104-10F02]
ATGGACATTGCCGAACTGCTTGCCTTCTCGGTGAAGAACAAGGCCTCGGACCTGCACTTGTCCGCCGGGCTGCCGCCGAT
GATTCGCGTCGATGGCGATGTCCGCCGGATCAACATCCCCGCGCTCGAACACAAGCAGGTGCACTCGCTGATCTACGACA
TCATGTCGGACAAGCAGCGGCGCGACTACGAGGAGTTCTACGAGACCGACTTCTCGTTCGAGATCCCCGGGCTGGCGCGC
TTCCGCGTCAATGCGTTCAACCAGAACCGCGGCGCCGGCGCGGTGTTCCGCACCATTCCCTCCGAGGTGCTGACGCTGGA
GGACCTGGGTTGCCCGCGCATCTTCAAGGAGCTGATCGAGCAGCCGCAGGGCCTGATCCTGGTGACCGGCCCGACCGGCT
CGGGCAAGTCGACCACGCTGGCGGCGATGGTCGACCACATCAACAAGAACGAATACGGGCACATGCTCACGATCGAGGAC
CCGATCGAGTTCGTGCACACCTCGCAGAAGTGCCTGGTCAACCAGCGCGAGATCCACCGCGACACGCTCGGCTTCAACGA
GGCGCTGCGTTCGGCGCTGCGCGAGGATCCCGACTACATCCTGGTCGGCGAGTTGCGCGACCTGGAGACCATCCGCCTGG
CGCTGACCGCGGCGGAAACCGGCCATCTGGTGTTCGCCACCGTGCACACCAGCTCGGCGGCGAAGACCATCGACCGCATC
ATCGACGTGTTCCCTGCCGGCGAGAAGCCGATGGTGCGTTCGATGCTGTCCGAAAGCCTGCGCGCGGTGATCTCGCAGTC
GCTGCTGAAGAAGGTCGGCGGTGGGCGCATCGCGGCGCACGAGATCATGGTCGGCATCCCGGCGATCCGCAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTACTCGTCGATTCAGACCGGCCAGCAGTTCGGCATGCAGACGCTGGACCAGTGCCTG
CAGGACCTGGTCAAGCGCGGCCTGGTGACGCGCCAGCAGGCGTCCGCCTACGCCAAGAACAAGGACAGCTTCAAGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB I4WZ44

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

78.488

99.71

0.783
  pilT Acinetobacter baumannii strain A118

78.488

99.71

0.783
  pilT Acinetobacter nosocomialis M2

78.488

99.71

0.783
  pilT Pseudomonas stutzeri DSM 10701

78.198

99.71

0.78
  pilT Pseudomonas aeruginosa PAK

77.907

99.71

0.777
  pilT Acinetobacter baylyi ADP1

77.326

99.71

0.771
  pilT Legionella pneumophila strain Lp02

74.419

99.71

0.742
  pilT Legionella pneumophila strain ERS1305867

74.419

99.71

0.742
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.048

97.391

0.672
  pilT Vibrio cholerae strain A1552

69.048

97.391

0.672
  pilT Neisseria meningitidis 8013

64.348

100

0.643
  pilT Neisseria gonorrhoeae MS11

64.058

100

0.641
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.116

100

0.481
  pilU Pseudomonas stutzeri DSM 10701

39.595

100

0.397
  pilU Acinetobacter baylyi ADP1

38.617

100

0.388
  pilU Vibrio cholerae strain A1552

38.728

100

0.388