Detailed information NTG0633945

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: LRK32_RS04240
• Genome accession: NZ_CP088920
• Coordinates: 950977..952143 (+)
• Length: 388 a.a.
• NCBI ID: WP_015446994.1
• Uniprot ID: -
• Organism: Rhodanobacter denitrificans strain FW104-10F02
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 945977..957143

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LRK32_RS04215 (LRK32_04215)	-	946097..946537 (+)	441	WP_015446991.1	secondary thiamine-phosphate synthase enzyme YjbQ	-
LRK32_RS04220 (LRK32_04220)	-	946542..947366 (+)	825	WP_037115662.1	cobalamin-binding protein	-
LRK32_RS04225 (LRK32_04225)	-	948021..948596 (-)	576	WP_007507063.1	YggT family protein	-
LRK32_RS04230 (LRK32_04230)	proC	948593..949417 (-)	825	WP_015446993.1	pyrroline-5-carboxylate reductase	-
LRK32_RS04235 (LRK32_04235)	pilT	949786..950823 (+)	1038	WP_007507061.1	type IV pilus twitching motility protein PilT	Machinery gene
LRK32_RS04240 (LRK32_04240)	pilU	950977..952143 (+)	1167	WP_015446994.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
LRK32_RS04245 (LRK32_04245)	-	952210..952587 (-)	378	WP_015446995.1	BON domain-containing protein	-
LRK32_RS04250 (LRK32_04250)	-	952940..955150 (-)	2211	WP_231378422.1	TonB-dependent siderophore receptor	-
LRK32_RS04255 (LRK32_04255)	-	955296..955988 (+)	693	WP_015446997.1	response regulator transcription factor	-

Sequence

Protein

• Length: 388 a.a.
• Molecular weight: 43408.83 Da
• Isoelectric Point: 6.7735

>NTDB_id=633945 LRK32_RS04240 WP_015446994.1 950977..952143(+) (pilU) [Rhodanobacter denitrificans strain FW104-10F02]
MSDFDFTSFLKLMVHKKASDLFITAGVAPSMKVQGRIVPITQSPLSVQQARDMVLNVMTPGQREEFEKTHECQFAISAQG
VGRFRVSCFYQRNCVGMVLRRIESKIPTIEELTLPPVIKQLAMTKRGIIVFVGATGSGKSTSLASMIGYRNQNSTGHIIT
IEDPIEYVHKHEGCIITQREVGIDTDSWDNALKNTLRQAPDVIMIGEIRTREGMDHAIAFAETGHLVLCTLHANNANQAM
DRILHFFPEDRRQQLLMDLSLNLKGIVAQQLIPTPDGKARRVAVEILLGTPLVQDYIRQGEIHKLKEVMKESTLLGMKTF
DQSLVELYHAGEISYEDALRYADSSNEVRLRIKLAQGGDAHTLSQGLEGVELEESRDAQNFSSNFLNR

Nucleotide

• Length: 1167 bp

>NTDB_id=633945 LRK32_RS04240 WP_015446994.1 950977..952143(+) (pilU) [Rhodanobacter denitrificans strain FW104-10F02]
ATGAGCGACTTCGATTTCACCTCATTCCTGAAGCTGATGGTGCACAAGAAGGCCTCCGACTTGTTCATCACGGCGGGCGT
CGCCCCCTCGATGAAAGTGCAGGGCCGGATCGTGCCGATCACGCAGAGCCCGCTCAGCGTGCAGCAGGCGCGCGACATGG
TGCTCAACGTGATGACGCCGGGCCAGCGCGAGGAGTTCGAGAAGACCCATGAGTGCCAGTTCGCGATCTCCGCGCAAGGC
GTGGGCCGTTTCCGCGTGTCGTGCTTCTACCAGCGCAACTGCGTGGGTATGGTGCTGCGCCGGATCGAGTCGAAGATCCC
CACCATCGAGGAACTGACGCTGCCGCCGGTGATCAAGCAACTGGCGATGACCAAGCGCGGCATCATCGTGTTCGTCGGCG
CCACCGGCTCGGGCAAATCGACCTCGCTGGCGTCGATGATCGGCTACCGCAACCAGAACTCGACCGGTCATATCATCACG
ATCGAGGACCCGATCGAGTACGTGCACAAGCACGAAGGCTGCATCATCACCCAGCGCGAGGTCGGCATCGACACCGACAG
CTGGGACAACGCGCTGAAGAACACCCTGCGCCAGGCCCCGGACGTGATCATGATCGGCGAAATCCGCACCCGCGAGGGCA
TGGACCACGCGATCGCCTTTGCCGAAACCGGTCACCTGGTGCTGTGCACGCTGCACGCGAACAACGCCAACCAGGCGATG
GACCGCATCCTGCACTTCTTCCCCGAGGACCGCCGCCAGCAACTGCTGATGGACCTGTCGCTCAACCTGAAGGGCATCGT
GGCGCAGCAGCTGATCCCCACGCCCGACGGCAAGGCGCGCCGGGTCGCGGTGGAGATTCTGCTGGGCACGCCGCTGGTGC
AGGACTACATCCGCCAGGGCGAGATCCACAAGCTGAAGGAGGTGATGAAGGAGTCCACTCTGCTCGGCATGAAGACCTTC
GACCAGAGCCTGGTCGAGCTGTACCACGCCGGCGAGATCAGCTACGAGGACGCGCTGCGCTACGCCGACAGCTCCAACGA
GGTGCGCCTGCGCATCAAGCTGGCCCAGGGCGGCGACGCGCATACCCTGTCGCAAGGCCTGGAAGGCGTCGAGCTGGAGG
AAAGCCGCGACGCGCAGAACTTCAGCAGCAATTTCCTCAACCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (116-272)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	66.578	96.392	0.642
pilU	Acinetobacter baylyi ADP1	63.483	91.753	0.582
pilU	Vibrio cholerae strain A1552	52.809	91.753	0.485
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.129	95.876	0.394