Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LQ771_RS03545 Genome accession   NZ_CP088252
Coordinates   775692..776729 (+) Length   345 a.a.
NCBI ID   WP_231351008.1    Uniprot ID   -
Organism   Frateuria soli strain 5GH9-11     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 770692..781729
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LQ771_RS03515 (LQ771_03515) - 771103..771933 (-) 831 WP_231351002.1 kinase -
  LQ771_RS03520 (LQ771_03520) pdxH 771973..772560 (+) 588 WP_231351003.1 pyridoxamine 5'-phosphate oxidase -
  LQ771_RS03525 (LQ771_03525) - 772557..772997 (+) 441 WP_231351004.1 secondary thiamine-phosphate synthase enzyme YjbQ -
  LQ771_RS03530 (LQ771_03530) - 772997..773794 (+) 798 WP_231351005.1 ABC transporter substrate-binding protein -
  LQ771_RS03535 (LQ771_03535) - 773924..774499 (-) 576 WP_231351006.1 YggT family protein -
  LQ771_RS03540 (LQ771_03540) proC 774496..775329 (-) 834 WP_231351007.1 pyrroline-5-carboxylate reductase -
  LQ771_RS03545 (LQ771_03545) pilT 775692..776729 (+) 1038 WP_231351008.1 type IV pilus twitching motility protein PilT Machinery gene
  LQ771_RS03550 (LQ771_03550) pilU 776864..778030 (+) 1167 WP_231351009.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LQ771_RS03555 (LQ771_03555) - 778230..778613 (-) 384 WP_231351010.1 BON domain-containing protein -
  LQ771_RS03560 (LQ771_03560) - 778886..780661 (-) 1776 WP_231351011.1 hypothetical protein -
  LQ771_RS03565 (LQ771_03565) - 780757..781683 (-) 927 WP_231351841.1 aspartate carbamoyltransferase catalytic subunit -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38586.20 Da        Isoelectric Point: 6.7297

>NTDB_id=633537 LQ771_RS03545 WP_231351008.1 775692..776729(+) (pilT) [Frateuria soli strain 5GH9-11]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPPLEHKQVHSLIYDIMSDKQRRDYEEFYETDFSFEIPGLAR
FRVNAFNQNRGAGAVFRTIPSEVLTLEDLGAPRIFRELIEQPQGLILVTGPTGSGKSTTLAAMVDHINKNEYGHMLTVED
PIEFVHTSQKCLVNQREVHRDTHGFNEALRSALREDPDYILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPSGEKPMVRSMLSESLRAVISQTLLKKVGGGRIAAHEIMVGIPAIRNLIREDKVAQMYSSIQTGQQYGMQTLDQNL
QDLVKRGLVARQEAIGYAKNKDIFK

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=633537 LQ771_RS03545 WP_231351008.1 775692..776729(+) (pilT) [Frateuria soli strain 5GH9-11]
ATGGACATCGCCGAACTGTTGGCCTTTTCGGTCAAGAACAAGGCCTCGGACCTGCATCTTTCGGCCGGCTTGCCGCCGAT
GATCCGCGTCGACGGCGACGTGCGTCGCATCAACATTCCGCCGCTGGAGCACAAGCAGGTCCATTCGCTGATCTACGACA
TCATGTCGGACAAGCAGCGGCGCGACTACGAGGAGTTCTACGAGACCGACTTCTCCTTCGAGATCCCCGGCCTGGCGCGC
TTCCGCGTCAACGCGTTCAACCAGAACCGCGGCGCCGGCGCGGTGTTCCGCACCATTCCCTCCGAGGTGCTGACGCTGGA
AGACCTCGGCGCACCGCGCATCTTCCGCGAGCTGATCGAGCAGCCGCAGGGCCTGATTCTGGTGACCGGCCCGACCGGCT
CGGGCAAGTCGACCACGCTGGCGGCGATGGTCGACCACATCAACAAGAACGAATACGGGCACATGCTCACGGTCGAGGAT
CCGATCGAGTTCGTGCACACCTCGCAGAAGTGCCTGGTCAACCAGCGCGAGGTGCATCGCGACACCCACGGATTCAACGA
GGCGCTCCGCTCGGCGCTGCGCGAGGACCCCGACTACATCCTGGTCGGCGAGCTGCGCGACCTGGAAACCATCCGCCTGG
CGCTGACCGCCGCGGAAACCGGCCACCTGGTGTTCGGCACCCTGCATACCAGCTCGGCGGCCAAGACCATCGACCGCATC
ATCGACGTGTTCCCTTCCGGCGAGAAGCCGATGGTGCGCTCGATGCTGTCCGAATCGCTGCGCGCGGTGATCTCGCAGAC
GCTGCTGAAGAAGGTCGGCGGCGGACGTATCGCGGCGCACGAGATCATGGTCGGCATCCCCGCCATCCGCAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTACTCGTCCATCCAGACCGGGCAGCAGTACGGCATGCAGACGCTGGACCAGAACCTG
CAGGACCTGGTCAAGCGCGGCCTGGTCGCGCGGCAGGAGGCGATCGGCTATGCCAAGAACAAGGACATATTCAAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

79.07

99.71

0.788
  pilT Acinetobacter baumannii D1279779

79.07

99.71

0.788
  pilT Acinetobacter baumannii strain A118

79.07

99.71

0.788
  pilT Pseudomonas stutzeri DSM 10701

78.198

99.71

0.78
  pilT Pseudomonas aeruginosa PAK

77.907

99.71

0.777
  pilT Acinetobacter baylyi ADP1

77.616

99.71

0.774
  pilT Legionella pneumophila strain Lp02

74.709

99.71

0.745
  pilT Legionella pneumophila strain ERS1305867

74.709

99.71

0.745
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.171

96.522

0.687
  pilT Vibrio cholerae strain A1552

71.171

96.522

0.687
  pilT Neisseria meningitidis 8013

64.723

99.42

0.643
  pilT Neisseria gonorrhoeae MS11

64.431

99.42

0.641
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50

95.652

0.478
  pilU Pseudomonas stutzeri DSM 10701

39.359

99.42

0.391
  pilU Vibrio cholerae strain A1552

39.359

99.42

0.391
  pilU Acinetobacter baylyi ADP1

38.824

98.551

0.383