Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LQ772_RS04225 Genome accession   NZ_CP088251
Coordinates   914640..915677 (+) Length   345 a.a.
NCBI ID   WP_231324242.1    Uniprot ID   -
Organism   Frateuria edaphi strain 5GH9-34     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 909640..920677
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LQ772_RS04195 (LQ772_04195) - 910111..910941 (-) 831 WP_231324231.1 kinase -
  LQ772_RS04200 (LQ772_04200) pdxH 910981..911568 (+) 588 WP_231324233.1 pyridoxamine 5'-phosphate oxidase -
  LQ772_RS04205 (LQ772_04205) - 911565..912005 (+) 441 WP_231324235.1 secondary thiamine-phosphate synthase enzyme YjbQ -
  LQ772_RS04210 (LQ772_04210) - 912005..912805 (+) 801 WP_231324237.1 ABC transporter substrate-binding protein -
  LQ772_RS04215 (LQ772_04215) - 912872..913447 (-) 576 WP_231324239.1 YggT family protein -
  LQ772_RS04220 (LQ772_04220) proC 913444..914277 (-) 834 WP_231324240.1 pyrroline-5-carboxylate reductase -
  LQ772_RS04225 (LQ772_04225) pilT 914640..915677 (+) 1038 WP_231324242.1 type IV pilus twitching motility protein PilT Machinery gene
  LQ772_RS04230 (LQ772_04230) pilU 915738..916904 (+) 1167 WP_231324244.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LQ772_RS04235 (LQ772_04235) - 917060..917443 (-) 384 WP_231324246.1 BON domain-containing protein -
  LQ772_RS04240 (LQ772_04240) - 917715..919490 (-) 1776 WP_231324248.1 hypothetical protein -
  LQ772_RS04245 (LQ772_04245) - 919587..920513 (-) 927 WP_231324249.1 aspartate carbamoyltransferase catalytic subunit -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38553.26 Da        Isoelectric Point: 6.7297

>NTDB_id=633516 LQ772_RS04225 WP_231324242.1 914640..915677(+) (pilT) [Frateuria edaphi strain 5GH9-34]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPPLEHKQVHSLIYDIMSDKQRRDYEEFYETDFSFEIPGLAR
FRVNAFNQNRGAGAVFRTIPSEVLTLEDLGAPRIFRELIEQPQGLILVTGPTGSGKSTTLAAMVDHINKNEYGHMLTVED
PIEFVHTSQKCLVNQREVHRDTHGFNEALRSALREDPDYILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAGEKPMVRSMLSESLRAVISQTLLKKVGGGRIAAHEIMVAVPAIRNLIREDKVAQMYSAIQTGQQYGMQTLDQIL
QDLVKRGLVARQEAIGYAKNKDIFK

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=633516 LQ772_RS04225 WP_231324242.1 914640..915677(+) (pilT) [Frateuria edaphi strain 5GH9-34]
ATGGACATCGCCGAACTGTTGGCCTTTTCGGTCAAGAACAAGGCCTCGGACCTGCATCTTTCGGCCGGCCTGCCGCCGAT
GATCCGCGTGGACGGCGACGTGCGTCGCATCAACATCCCGCCGCTGGAGCACAAGCAGGTCCATTCGCTGATCTACGACA
TCATGTCGGACAAGCAGCGGCGCGACTACGAAGAGTTCTACGAGACCGACTTCTCGTTCGAGATTCCCGGCCTGGCGCGC
TTCCGCGTCAACGCGTTCAACCAGAACCGCGGCGCCGGCGCGGTGTTCCGCACGATTCCATCCGAAGTGCTGACCCTGGA
AGACCTCGGCGCGCCGCGCATCTTCCGCGAGCTGATCGAACAGCCGCAGGGGCTGATCCTGGTCACCGGCCCGACCGGTT
CGGGCAAGTCGACCACGCTGGCGGCGATGGTCGACCACATCAACAAGAACGAATACGGGCACATGCTCACGGTCGAAGAT
CCGATCGAGTTCGTGCATACCTCGCAGAAGTGCCTGGTCAACCAGCGCGAGGTGCATCGCGACACCCACGGCTTCAACGA
GGCGTTGCGCTCGGCGCTGCGCGAAGATCCCGACTACATCCTGGTCGGCGAGTTGCGCGACCTGGAAACCATCCGCCTGG
CGCTGACCGCCGCGGAAACCGGTCACCTGGTGTTCGGCACCCTGCACACCAGCTCGGCGGCCAAGACCATCGACCGCATC
ATCGACGTGTTCCCGGCCGGCGAGAAGCCGATGGTGCGCTCGATGCTCTCCGAATCGTTGCGCGCGGTGATTTCCCAGAC
GCTGCTCAAGAAGGTCGGCGGCGGGCGAATCGCGGCGCACGAGATCATGGTCGCGGTCCCTGCGATCCGCAACCTGATCC
GCGAGGACAAGGTCGCGCAGATGTACTCGGCGATCCAGACCGGCCAGCAGTACGGCATGCAGACGCTGGACCAGATCCTG
CAGGATCTCGTCAAGCGCGGCCTGGTCGCGCGGCAGGAAGCCATCGGGTATGCCAAGAACAAGGACATTTTCAAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

79.07

99.71

0.788
  pilT Acinetobacter baumannii D1279779

79.07

99.71

0.788
  pilT Acinetobacter baumannii strain A118

79.07

99.71

0.788
  pilT Pseudomonas aeruginosa PAK

78.198

99.71

0.78
  pilT Pseudomonas stutzeri DSM 10701

77.907

99.71

0.777
  pilT Acinetobacter baylyi ADP1

77.035

99.71

0.768
  pilT Legionella pneumophila strain Lp02

74.709

99.71

0.745
  pilT Legionella pneumophila strain ERS1305867

74.709

99.71

0.745
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.171

96.522

0.687
  pilT Vibrio cholerae strain A1552

71.171

96.522

0.687
  pilT Neisseria meningitidis 8013

65.306

99.42

0.649
  pilT Neisseria gonorrhoeae MS11

65.015

99.42

0.646
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.303

95.652

0.481
  pilU Pseudomonas stutzeri DSM 10701

39.65

99.42

0.394
  pilU Vibrio cholerae strain A1552

39.359

99.42

0.391
  pilU Acinetobacter baylyi ADP1

39.118

98.551

0.386