Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   LQ772_RS04230 Genome accession   NZ_CP088251
Coordinates   915738..916904 (+) Length   388 a.a.
NCBI ID   WP_231324244.1    Uniprot ID   -
Organism   Frateuria edaphi strain 5GH9-34     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 910738..921904
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LQ772_RS04200 (LQ772_04200) pdxH 910981..911568 (+) 588 WP_231324233.1 pyridoxamine 5'-phosphate oxidase -
  LQ772_RS04205 (LQ772_04205) - 911565..912005 (+) 441 WP_231324235.1 secondary thiamine-phosphate synthase enzyme YjbQ -
  LQ772_RS04210 (LQ772_04210) - 912005..912805 (+) 801 WP_231324237.1 ABC transporter substrate-binding protein -
  LQ772_RS04215 (LQ772_04215) - 912872..913447 (-) 576 WP_231324239.1 YggT family protein -
  LQ772_RS04220 (LQ772_04220) proC 913444..914277 (-) 834 WP_231324240.1 pyrroline-5-carboxylate reductase -
  LQ772_RS04225 (LQ772_04225) pilT 914640..915677 (+) 1038 WP_231324242.1 type IV pilus twitching motility protein PilT Machinery gene
  LQ772_RS04230 (LQ772_04230) pilU 915738..916904 (+) 1167 WP_231324244.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LQ772_RS04235 (LQ772_04235) - 917060..917443 (-) 384 WP_231324246.1 BON domain-containing protein -
  LQ772_RS04240 (LQ772_04240) - 917715..919490 (-) 1776 WP_231324248.1 hypothetical protein -
  LQ772_RS04245 (LQ772_04245) - 919587..920513 (-) 927 WP_231324249.1 aspartate carbamoyltransferase catalytic subunit -
  LQ772_RS04250 (LQ772_04250) ruvX 920568..920999 (-) 432 WP_231324251.1 Holliday junction resolvase RuvX -
  LQ772_RS04255 (LQ772_04255) - 921037..921609 (-) 573 WP_231324253.1 YqgE/AlgH family protein -

Sequence

Protein


Download         Length: 388 a.a.        Molecular weight: 43472.84 Da        Isoelectric Point: 6.9194

>NTDB_id=633517 LQ772_RS04230 WP_231324244.1 915738..916904(+) (pilU) [Frateuria edaphi strain 5GH9-34]
MSDFDFTSFLKLMVHKKASDLFVTAGVAPSMKLQGRVVPITQSPLSVQQARDMVLNIMTPAQREEFEKTHECQFAISAQG
VGRFRVSCFYQRNCVGMVLRRIESKIPTFEELTLPPVLKTLSMTKRGIIIFVGATGSGKSTSLAAMVGYRNQNSTGHIIT
IEDPIEYVHKHEGCIITQRELGIDTDSWDNALKNTLRQAPDVILIGEVRTRETMEYAINFSETGHLCLCTLHANNANQAI
DRILHFFPEDRRQQLFMDLSLNLKGIVAQQLIPTPDGKARRVAVEVLLGTPLVQDYIRQGEIHKIKEVMKDSVNLGMRTF
DQSLVELYHAGEISYEDALRHADSSNEVRLRIKLAQGGDAHTLSQGLEGVELEDTRDNSQFGGGMLRR

Nucleotide


Download         Length: 1167 bp        

>NTDB_id=633517 LQ772_RS04230 WP_231324244.1 915738..916904(+) (pilU) [Frateuria edaphi strain 5GH9-34]
ATGAGCGATTTCGACTTCACCTCCTTCCTCAAGCTGATGGTGCACAAGAAGGCATCGGACCTGTTCGTCACCGCCGGCGT
GGCGCCGTCGATGAAGCTGCAGGGCCGCGTCGTGCCGATCACGCAGAGCCCGCTGTCCGTGCAGCAGGCGCGCGACATGG
TGCTCAACATCATGACGCCGGCGCAGCGCGAGGAGTTCGAGAAGACCCACGAGTGCCAGTTCGCGATCTCAGCGCAGGGC
GTGGGCCGCTTCCGCGTGTCGTGCTTCTACCAGCGCAACTGCGTGGGAATGGTGCTGCGCCGGATCGAGTCGAAGATCCC
GACCTTCGAGGAGCTCACGCTGCCGCCGGTGCTCAAGACGCTGTCGATGACCAAGCGCGGCATCATCATCTTCGTCGGCG
CCACCGGTTCGGGTAAGTCGACCTCGCTGGCCGCGATGGTGGGTTACCGCAACCAGAATTCGACCGGCCACATCATCACG
ATCGAAGACCCGATCGAGTACGTGCACAAGCACGAGGGCTGCATCATTACCCAGCGCGAGCTCGGCATCGACACCGACAG
CTGGGACAACGCGCTGAAGAACACCCTGCGCCAGGCGCCCGACGTGATCCTGATCGGCGAGGTGCGTACCCGCGAGACGA
TGGAGTACGCGATCAACTTCTCCGAGACCGGACACCTGTGCCTGTGCACGCTGCATGCGAACAACGCCAACCAGGCGATC
GACCGCATCCTGCATTTCTTCCCCGAAGACCGGCGCCAGCAGTTGTTCATGGACCTGTCGCTGAACCTCAAGGGCATCGT
GGCGCAGCAGCTGATCCCCACGCCCGACGGCAAGGCGCGGCGCGTGGCAGTCGAGGTGCTGCTGGGCACGCCGCTGGTGC
AGGACTACATCCGCCAGGGCGAGATCCACAAGATCAAGGAAGTGATGAAGGACTCGGTCAACCTCGGCATGCGCACGTTC
GACCAGAGCCTGGTCGAGCTCTATCACGCCGGCGAGATCAGCTACGAGGACGCACTTCGCCACGCCGACAGCTCCAACGA
GGTGCGCTTGCGCATCAAGCTGGCCCAGGGCGGCGATGCGCACACCTTGTCCCAGGGTCTGGAAGGCGTAGAGCTGGAAG
ATACCCGCGACAACAGCCAGTTCGGCGGCGGCATGCTCCGGCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

64.533

96.649

0.624
  pilU Acinetobacter baylyi ADP1

62.745

92.01

0.577
  pilU Vibrio cholerae strain A1552

53.371

91.753

0.49
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.062

98.969

0.387