Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LPA45_RS14395 Genome accession   NZ_CP088083
Coordinates   3078824..3079867 (-) Length   347 a.a.
NCBI ID   WP_116318086.1    Uniprot ID   -
Organism   Cupriavidus sp. Agwp_2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3073824..3084867
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LPA45_RS14365 (LPA45_14370) - 3074762..3075139 (+) 378 WP_116318080.1 CidA/LrgA family protein -
  LPA45_RS14370 (LPA45_14375) - 3075136..3075861 (+) 726 WP_116318081.1 LrgB family protein -
  LPA45_RS14375 (LPA45_14380) - 3075947..3076318 (+) 372 WP_199414524.1 VOC family protein -
  LPA45_RS14380 (LPA45_14385) - 3076334..3077023 (+) 690 WP_116318083.1 YafY family protein -
  LPA45_RS14385 (LPA45_14390) - 3077026..3077520 (-) 495 WP_317920414.1 glutathione peroxidase -
  LPA45_RS14390 (LPA45_14395) pilU 3077596..3078735 (-) 1140 WP_116318085.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LPA45_RS14395 (LPA45_14400) pilT 3078824..3079867 (-) 1044 WP_116318086.1 type IV pilus twitching motility protein PilT Machinery gene
  LPA45_RS14400 (LPA45_14405) - 3079935..3080624 (+) 690 WP_317920412.1 YggS family pyridoxal phosphate-dependent enzyme -
  LPA45_RS14405 (LPA45_14410) proC 3080664..3081497 (+) 834 WP_116318088.1 pyrroline-5-carboxylate reductase -
  LPA45_RS14410 (LPA45_14415) ubiA 3082484..3083341 (-) 858 WP_317922947.1 4-hydroxybenzoate octaprenyltransferase -
  LPA45_RS14415 (LPA45_14420) - 3083483..3083968 (-) 486 WP_010815054.1 Dps family protein -
  LPA45_RS14420 (LPA45_14425) - 3084062..3084256 (-) 195 WP_116318090.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38405.20 Da        Isoelectric Point: 6.9772

>NTDB_id=632625 LPA45_RS14395 WP_116318086.1 3078824..3079867(-) (pilT) [Cupriavidus sp. Agwp_2]
MDIAQLLAFAVKNKASDLHLSSDMPPMVRIHGDMRRINVAAMAHKDVHAMVYDIMSDTQRKIYEERLEIDFSFEIAGLSR
FRVNAYNTQRGAAAVFRTIPSKVLTLEELRAPAVFADLCMKPRGLVLVTGPTGSGKSTTLAAMVDHRNENDMGHILTVED
PIEFVHNSKKSLINQRELGPHTHSFANALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRV
VDVFPPEEKDMVRTMLSESLEAVISQTLLKTRDGNGRTAAHEIMIATPAIRHLIRENKIAQMYSMMQTSSGLGMQTLDQC
LSDLIKRSAISYNDARAIAKNPDAFMG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=632625 LPA45_RS14395 WP_116318086.1 3078824..3079867(-) (pilT) [Cupriavidus sp. Agwp_2]
ATGGACATCGCGCAGCTATTGGCTTTCGCCGTCAAGAACAAGGCGTCCGATCTTCATCTGTCATCGGACATGCCGCCGAT
GGTCCGGATCCACGGCGACATGCGCCGGATCAATGTCGCGGCGATGGCGCACAAGGATGTCCACGCCATGGTGTACGACA
TCATGAGCGACACCCAGCGCAAGATCTACGAAGAACGGCTGGAGATCGACTTCTCGTTCGAGATCGCCGGCCTGTCGCGC
TTCCGGGTCAATGCCTACAACACCCAGCGCGGCGCCGCCGCGGTGTTCCGTACCATTCCCTCCAAGGTCCTGACGCTGGA
AGAGCTGCGCGCGCCGGCGGTGTTCGCCGACCTGTGCATGAAACCGCGCGGGCTGGTGCTGGTGACCGGGCCGACCGGCT
CGGGCAAGTCAACCACGCTGGCGGCGATGGTCGACCATCGCAATGAAAACGACATGGGTCACATCCTGACGGTGGAAGAC
CCGATCGAATTCGTCCACAACTCCAAGAAGAGCCTGATCAACCAGCGCGAGCTGGGGCCGCACACGCATTCGTTCGCCAA
TGCGCTGCGCTCGGCGCTGCGCGAGGACCCGGACGTGATTCTTGTGGGCGAGTTGCGCGACCTGGAGACCATCCGCCTGG
CGCTGACCGCGGCCGAGACCGGCCACCTGGTCTTCGGCACGCTGCACACCAGCTCGGCGGCCAAGACCATCGACCGCGTG
GTCGACGTGTTCCCGCCGGAAGAGAAGGACATGGTGCGCACCATGCTGTCGGAATCGCTGGAAGCGGTGATCTCGCAGAC
ACTGCTGAAGACCCGCGACGGCAACGGCCGCACTGCCGCGCACGAGATCATGATCGCCACGCCGGCGATCCGGCACCTGA
TCCGCGAGAACAAGATCGCGCAGATGTACTCGATGATGCAGACCAGCAGCGGGCTGGGCATGCAGACGCTGGACCAGTGC
CTGTCGGACCTGATCAAGCGCAGCGCCATCAGCTACAACGACGCGCGCGCCATCGCGAAGAACCCCGACGCGTTCATGGG
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

71.676

99.712

0.715
  pilT Acinetobacter baumannii D1279779

71.676

99.712

0.715
  pilT Acinetobacter baumannii strain A118

71.676

99.712

0.715
  pilT Pseudomonas stutzeri DSM 10701

70.435

99.424

0.7
  pilT Acinetobacter baylyi ADP1

69.565

99.424

0.692
  pilT Pseudomonas aeruginosa PAK

69.565

99.424

0.692
  pilT Neisseria gonorrhoeae MS11

67.826

99.424

0.674
  pilT Neisseria meningitidis 8013

67.826

99.424

0.674
  pilT Legionella pneumophila strain Lp02

66.667

99.424

0.663
  pilT Legionella pneumophila strain ERS1305867

66.667

99.424

0.663
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

65.282

97.118

0.634
  pilT Vibrio cholerae strain A1552

65.282

97.118

0.634
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.565

99.424

0.493
  pilU Acinetobacter baylyi ADP1

39.826

99.135

0.395
  pilU Vibrio cholerae strain A1552

40.597

96.542

0.392
  pilU Pseudomonas stutzeri DSM 10701

40.299

96.542

0.389