Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   LPA45_RS14390 Genome accession   NZ_CP088083
Coordinates   3077596..3078735 (-) Length   379 a.a.
NCBI ID   WP_116318085.1    Uniprot ID   -
Organism   Cupriavidus sp. Agwp_2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3072596..3083735
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LPA45_RS14360 (LPA45_14365) glcF 3073423..3074676 (+) 1254 WP_116318079.1 glycolate oxidase subunit GlcF -
  LPA45_RS14365 (LPA45_14370) - 3074762..3075139 (+) 378 WP_116318080.1 CidA/LrgA family protein -
  LPA45_RS14370 (LPA45_14375) - 3075136..3075861 (+) 726 WP_116318081.1 LrgB family protein -
  LPA45_RS14375 (LPA45_14380) - 3075947..3076318 (+) 372 WP_199414524.1 VOC family protein -
  LPA45_RS14380 (LPA45_14385) - 3076334..3077023 (+) 690 WP_116318083.1 YafY family protein -
  LPA45_RS14385 (LPA45_14390) - 3077026..3077520 (-) 495 WP_317920414.1 glutathione peroxidase -
  LPA45_RS14390 (LPA45_14395) pilU 3077596..3078735 (-) 1140 WP_116318085.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LPA45_RS14395 (LPA45_14400) pilT 3078824..3079867 (-) 1044 WP_116318086.1 type IV pilus twitching motility protein PilT Machinery gene
  LPA45_RS14400 (LPA45_14405) - 3079935..3080624 (+) 690 WP_317920412.1 YggS family pyridoxal phosphate-dependent enzyme -
  LPA45_RS14405 (LPA45_14410) proC 3080664..3081497 (+) 834 WP_116318088.1 pyrroline-5-carboxylate reductase -
  LPA45_RS14410 (LPA45_14415) ubiA 3082484..3083341 (-) 858 WP_317922947.1 4-hydroxybenzoate octaprenyltransferase -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 42200.43 Da        Isoelectric Point: 6.5767

>NTDB_id=632624 LPA45_RS14390 WP_116318085.1 3077596..3078735(-) (pilU) [Cupriavidus sp. Agwp_2]
MLDRESAAKYINDLLELMVSNRGSDLFITSDFPPAIKVDGKITPVSQQPLNPTQALGLVRSVMNERQVQDFDTSRECNFA
ITAPKAGRFRVSAFIQQGKAGMVVRTINTRIPSVADLDLPPTLHDIVMSKRGLVIVTGATGSGKSTTLAAMLDHRNAQSY
GHIITIEDPIEYVHAHQNCIVTQREVGIDTESWHVALKNTLRQAPDVILIGEIRDRETMEYAMQYAETGHLCLATLHANN
ANQAIDRVVNFFPEEKRQQLLIDLSLNLKAMVSQRLLPRAGRKGRVPAVEIMIGTPLVADLIFKGEIHELKEVIKKSREQ
GMISFDQALFDLYEEGKITYEDALRNADSLNDLRLMIKLHSARARDTDLGAGTEHLNVI

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=632624 LPA45_RS14390 WP_116318085.1 3077596..3078735(-) (pilU) [Cupriavidus sp. Agwp_2]
ATGCTCGATCGCGAATCCGCCGCCAAGTACATCAACGACCTGCTGGAGCTGATGGTCAGCAACCGCGGCTCGGACCTGTT
CATCACTTCCGACTTCCCGCCGGCGATCAAGGTGGACGGCAAGATCACGCCGGTGTCGCAGCAGCCGCTGAACCCGACGC
AGGCGCTGGGCCTGGTGCGCTCGGTGATGAACGAGCGGCAGGTGCAGGATTTCGATACCAGCCGCGAATGCAACTTCGCC
ATCACCGCGCCCAAGGCCGGGCGCTTCCGCGTGTCGGCGTTTATCCAGCAGGGCAAGGCCGGCATGGTGGTGCGGACCAT
CAATACGCGGATTCCGTCCGTGGCGGACCTGGACCTGCCCCCGACGCTGCACGACATCGTGATGTCCAAGCGCGGGCTGG
TGATCGTGACCGGCGCGACCGGCTCGGGCAAGTCGACCACGCTGGCTGCCATGCTCGACCACCGCAATGCGCAGTCCTAT
GGCCACATCATCACCATCGAGGATCCGATCGAATACGTGCACGCGCACCAGAACTGCATCGTCACGCAGCGCGAGGTCGG
CATCGATACGGAATCCTGGCACGTGGCGCTGAAGAACACGCTGCGCCAGGCGCCCGACGTGATCCTGATCGGCGAAATCC
GCGACCGCGAGACCATGGAATACGCGATGCAGTACGCCGAAACCGGCCACCTGTGCCTGGCCACGCTGCACGCCAACAAT
GCCAACCAGGCGATCGACCGCGTGGTCAACTTCTTCCCCGAGGAAAAGCGCCAGCAGCTGCTGATCGACCTGTCGCTGAA
CCTGAAGGCGATGGTGTCGCAGCGCCTGCTGCCGCGCGCCGGGCGCAAGGGCCGCGTGCCGGCGGTGGAAATCATGATCG
GCACGCCGCTGGTGGCAGACCTGATCTTCAAGGGCGAGATCCACGAGCTGAAGGAAGTCATCAAGAAATCGCGCGAGCAG
GGCATGATCTCGTTCGACCAGGCGCTGTTCGACCTGTACGAGGAGGGCAAGATCACCTATGAGGATGCGCTGCGCAACGC
AGATTCGCTCAATGACCTGCGCCTGATGATCAAGCTGCACAGCGCGCGCGCCAGGGACACCGACCTCGGCGCAGGCACCG
AGCACCTGAACGTCATATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(54-278)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

63.218

91.821

0.58
  pilU Acinetobacter baylyi ADP1

57.714

92.348

0.533
  pilU Vibrio cholerae strain A1552

52.893

95.778

0.507
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.104

88.918

0.401
  pilT Pseudomonas stutzeri DSM 10701

43.323

88.918

0.385
  pilT Pseudomonas aeruginosa PAK

42.73

88.918

0.38
  pilT Acinetobacter baumannii D1279779

43.243

87.863

0.38
  pilT Acinetobacter nosocomialis M2

43.243

87.863

0.38
  pilT Acinetobacter baumannii strain A118

43.243

87.863

0.38
  pilT Legionella pneumophila strain Lp02

42.643

87.863

0.375
  pilT Acinetobacter baylyi ADP1

42.643

87.863

0.375
  pilT Legionella pneumophila strain ERS1305867

42.643

87.863

0.375
  pilT Neisseria meningitidis 8013

40.588

89.71

0.364