Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LOC50_RS11320 Genome accession   NZ_CP087995
Coordinates   2398599..2399639 (-) Length   346 a.a.
NCBI ID   WP_013465851.1    Uniprot ID   A0AA37S294
Organism   Pseudoalteromonas sp. SCSIO 43095     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2393599..2404639
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LOC50_RS11290 (LOC50_11280) - 2393954..2394423 (+) 470 Protein_2161 hypothetical protein -
  LOC50_RS11295 (LOC50_11285) rsmE 2394495..2395229 (+) 735 WP_069439861.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  LOC50_RS11300 (LOC50_11290) gshB 2395296..2396246 (+) 951 WP_013465847.1 glutathione synthase -
  LOC50_RS11305 (LOC50_11295) - 2396382..2396939 (+) 558 WP_013465848.1 YqgE/AlgH family protein -
  LOC50_RS11310 (LOC50_11300) ruvX 2396957..2397406 (+) 450 WP_013465849.1 Holliday junction resolvase RuvX -
  LOC50_RS11315 (LOC50_11305) pilU 2397459..2398589 (-) 1131 WP_036980184.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LOC50_RS11320 (LOC50_11310) pilT 2398599..2399639 (-) 1041 WP_013465851.1 type IV pilus twitching motility protein PilT Machinery gene
  LOC50_RS11325 (LOC50_11315) - 2399642..2400355 (+) 714 WP_069439860.1 YggS family pyridoxal phosphate-dependent enzyme -
  LOC50_RS11330 (LOC50_11320) proC 2400373..2401194 (+) 822 WP_036980188.1 pyrroline-5-carboxylate reductase -
  LOC50_RS11335 (LOC50_11325) - 2401221..2401754 (+) 534 WP_069439859.1 YggT family protein -
  LOC50_RS11340 (LOC50_11330) - 2401812..2402255 (+) 444 WP_036980192.1 DUF4426 domain-containing protein -
  LOC50_RS11345 (LOC50_11335) - 2402258..2402857 (+) 600 WP_069439858.1 XTP/dITP diphosphatase -
  LOC50_RS11350 (LOC50_11340) hemW 2402854..2403990 (+) 1137 WP_069439857.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38244.49 Da        Isoelectric Point: 6.2312

>NTDB_id=632186 LOC50_RS11320 WP_013465851.1 2398599..2399639(-) (pilT) [Pseudoalteromonas sp. SCSIO 43095]
MDITELLAFSVQHKASDLHLSSGVSPMIRVDGDVRRINIPALGDKEVSSLVYDIMNDNQRKDYEQNLEVDFSFEVPNLAR
FRVNAFNSNRGPAAVFRTIPSDVLTLEDLGAPEIFKQISDNPRGLVLVTGPTGSGKSTTLAAMVDYINQTKHHHVLTIED
PIEFVHDNKLSLINQREVHRDTHSFSNALRSALREDPDVILVGELRDLETIRLAMTAAETGHLVFGTLHTTSAPKTIDRI
IDVFPGEEKDMIRSMLSESLRAVISQTLIKKVGGGRVAAHEIMLATPAIRNLIREDKIAQMYSSIQTGASQGMQTMDQCL
TSLVNRGIITHSAAYTKAQDKTQFSA

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=632186 LOC50_RS11320 WP_013465851.1 2398599..2399639(-) (pilT) [Pseudoalteromonas sp. SCSIO 43095]
ATGGATATCACCGAATTATTGGCATTTAGTGTGCAACATAAAGCATCAGATTTGCACTTATCATCAGGGGTATCACCTAT
GATACGTGTTGATGGCGATGTGCGACGTATTAATATACCAGCACTTGGAGATAAAGAAGTCAGCAGTCTGGTTTATGACA
TTATGAACGATAACCAGCGTAAGGACTATGAGCAAAATCTCGAAGTGGATTTTTCTTTTGAAGTCCCTAATTTAGCACGT
TTTCGTGTAAATGCCTTTAACTCTAATCGTGGCCCTGCTGCTGTGTTTCGTACTATCCCGAGTGACGTACTCACTCTTGA
AGATTTAGGCGCACCTGAAATATTTAAACAAATTTCAGATAACCCACGAGGCCTAGTGTTAGTCACAGGCCCTACAGGTT
CAGGTAAGTCGACCACGCTTGCGGCGATGGTCGATTACATTAACCAAACTAAGCATCATCATGTGCTGACTATTGAAGAT
CCTATCGAATTTGTTCATGATAATAAACTCAGCTTAATTAATCAGCGGGAAGTTCATCGTGATACACATAGCTTTTCTAA
TGCACTACGCAGCGCACTTCGTGAAGACCCTGATGTTATTTTGGTTGGTGAGCTACGAGACTTAGAAACTATTCGCCTTG
CTATGACCGCTGCGGAAACGGGTCACTTGGTGTTTGGTACTTTGCATACGACCTCTGCACCTAAAACCATTGACCGTATT
ATTGATGTATTCCCTGGCGAAGAAAAAGATATGATCCGCTCTATGCTCTCTGAGTCATTACGTGCAGTTATTTCGCAAAC
ACTGATAAAAAAAGTAGGGGGAGGGCGAGTGGCTGCACATGAAATTATGTTGGCAACGCCTGCTATTCGTAATTTAATCC
GTGAAGATAAAATTGCACAAATGTACTCATCAATTCAAACCGGTGCGTCGCAAGGCATGCAAACCATGGATCAATGTTTA
ACAAGCTTAGTTAACCGTGGCATTATCACTCATTCAGCGGCATATACAAAAGCGCAAGATAAGACACAGTTTAGCGCTTA
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

76.453

99.422

0.76
  pilT Pseudomonas aeruginosa PAK

75.872

99.422

0.754
  pilT Acinetobacter nosocomialis M2

75.872

99.422

0.754
  pilT Acinetobacter baumannii D1279779

75.872

99.422

0.754
  pilT Acinetobacter baumannii strain A118

75.872

99.422

0.754
  pilT Acinetobacter baylyi ADP1

75.291

99.422

0.749
  pilT Legionella pneumophila strain Lp02

71.802

99.422

0.714
  pilT Legionella pneumophila strain ERS1305867

71.802

99.422

0.714
  pilT Neisseria gonorrhoeae MS11

69.942

100

0.699
  pilT Neisseria meningitidis 8013

69.942

100

0.699
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

73.252

95.087

0.697
  pilT Vibrio cholerae strain A1552

73.252

95.087

0.697
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.312

100

0.523
  pilU Vibrio cholerae strain A1552

42.154

93.931

0.396
  pilU Acinetobacter baylyi ADP1

38.824

98.266

0.382
  pilU Pseudomonas stutzeri DSM 10701

38.643

97.977

0.379