Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   LOC50_RS11315 Genome accession   NZ_CP087995
Coordinates   2397459..2398589 (-) Length   376 a.a.
NCBI ID   WP_036980184.1    Uniprot ID   -
Organism   Pseudoalteromonas sp. SCSIO 43095     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2392459..2403589
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LOC50_RS11285 (LOC50_11275) - 2392983..2393876 (+) 894 WP_036980180.1 hypothetical protein -
  LOC50_RS11290 (LOC50_11280) - 2393954..2394423 (+) 470 Protein_2161 hypothetical protein -
  LOC50_RS11295 (LOC50_11285) rsmE 2394495..2395229 (+) 735 WP_069439861.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  LOC50_RS11300 (LOC50_11290) gshB 2395296..2396246 (+) 951 WP_013465847.1 glutathione synthase -
  LOC50_RS11305 (LOC50_11295) - 2396382..2396939 (+) 558 WP_013465848.1 YqgE/AlgH family protein -
  LOC50_RS11310 (LOC50_11300) ruvX 2396957..2397406 (+) 450 WP_013465849.1 Holliday junction resolvase RuvX -
  LOC50_RS11315 (LOC50_11305) pilU 2397459..2398589 (-) 1131 WP_036980184.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LOC50_RS11320 (LOC50_11310) pilT 2398599..2399639 (-) 1041 WP_013465851.1 type IV pilus twitching motility protein PilT Machinery gene
  LOC50_RS11325 (LOC50_11315) - 2399642..2400355 (+) 714 WP_069439860.1 YggS family pyridoxal phosphate-dependent enzyme -
  LOC50_RS11330 (LOC50_11320) proC 2400373..2401194 (+) 822 WP_036980188.1 pyrroline-5-carboxylate reductase -
  LOC50_RS11335 (LOC50_11325) - 2401221..2401754 (+) 534 WP_069439859.1 YggT family protein -
  LOC50_RS11340 (LOC50_11330) - 2401812..2402255 (+) 444 WP_036980192.1 DUF4426 domain-containing protein -
  LOC50_RS11345 (LOC50_11335) - 2402258..2402857 (+) 600 WP_069439858.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41732.74 Da        Isoelectric Point: 6.2895

>NTDB_id=632185 LOC50_RS11315 WP_036980184.1 2397459..2398589(-) (pilU) [Pseudoalteromonas sp. SCSIO 43095]
MTLSLNHFLQIMVDKKGSDLFVSSQLPISAKINGELTPLNDDKLTDEQALELVESAMSEKQKAEFHNTKECNFAVATSEG
RFRISAFWQRDRAGMVIRRIVTKIPDVKELGLPSALTDVIMSKRGLVLFVGGTGTGKSTSLAALIGYRNRNQRGHILTIE
DPIEFVHEHQKSIITQREVGLDTESFESALKSSLRQAPDVILIGEIRSQETMEYALSFAETGHLCVATLHANNANQAIDR
IMHLVPKEKHDKLKYDLALNLRAIIAQQLIPTSDGEGRVAAIEVLLNTPMVSELIKNGNIGGIKETMAKSTEMGMQTFDQ
ALFELYRQQRINYADALHHADSPNDLRLMIKLRNNEQQGAGFLQGVTIDGLDDKGI

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=632185 LOC50_RS11315 WP_036980184.1 2397459..2398589(-) (pilU) [Pseudoalteromonas sp. SCSIO 43095]
ATGACTTTATCTTTAAATCACTTTTTACAAATAATGGTCGACAAAAAAGGCTCTGATTTATTTGTATCGAGCCAACTTCC
TATTAGCGCTAAAATTAACGGCGAGTTAACTCCTCTTAATGACGATAAATTAACAGATGAGCAAGCGCTGGAATTAGTCG
AATCAGCGATGAGTGAAAAACAAAAAGCAGAGTTTCATAACACTAAAGAATGTAACTTTGCGGTTGCAACTAGCGAAGGG
CGCTTTCGTATTTCGGCTTTTTGGCAACGCGACAGAGCGGGCATGGTTATTCGCCGAATCGTCACTAAAATTCCCGACGT
TAAAGAGCTTGGATTACCTTCTGCATTGACCGATGTGATTATGTCAAAGCGCGGACTCGTGTTATTTGTGGGTGGTACGG
GAACCGGTAAATCAACGTCTTTGGCGGCACTTATTGGGTATAGAAATCGCAATCAACGTGGCCATATTTTAACCATAGAA
GATCCGATTGAATTTGTGCATGAGCATCAAAAAAGTATTATTACCCAACGTGAAGTGGGTTTAGATACTGAAAGCTTTGA
ATCAGCACTTAAAAGCTCTTTGCGCCAAGCGCCTGATGTTATTTTAATTGGTGAAATTCGTTCGCAAGAAACCATGGAAT
ATGCGTTGAGCTTTGCAGAAACGGGTCATTTATGTGTTGCCACCCTGCATGCTAACAATGCTAACCAAGCGATTGACCGT
ATTATGCATTTGGTACCAAAAGAAAAGCACGATAAGCTTAAATATGACTTAGCATTAAATTTACGCGCTATCATTGCTCA
GCAACTTATTCCGACCTCTGATGGGGAAGGGCGTGTTGCTGCCATTGAGGTATTACTTAATACGCCAATGGTCAGTGAGC
TAATTAAAAACGGTAATATTGGTGGTATTAAAGAAACCATGGCCAAATCAACAGAGATGGGCATGCAAACCTTTGACCAA
GCGCTGTTTGAGCTTTACAGGCAGCAACGTATTAATTATGCCGATGCGCTGCATCATGCGGATTCGCCAAATGACTTACG
CTTGATGATTAAACTACGTAATAACGAGCAGCAAGGCGCAGGCTTTTTGCAAGGTGTGACCATTGATGGCCTTGATGACA
AAGGCATATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(14-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

57.6

99.734

0.574
  pilU Acinetobacter baylyi ADP1

57.294

100

0.574
  pilU Vibrio cholerae strain A1552

56.403

97.606

0.551
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.944

95.745

0.402