Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LPH35_RS02865 Genome accession   NZ_CP087711
Coordinates   629913..631013 (-) Length   366 a.a.
NCBI ID   WP_152109238.1    Uniprot ID   -
Organism   Plesiomonas shigelloides strain 7A     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 624913..636013
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LPH35_RS02835 rsmE 625372..626106 (+) 735 WP_010864547.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  LPH35_RS02840 gshB 626116..627069 (+) 954 WP_047708158.1 glutathione synthase -
  LPH35_RS02845 - 627283..627846 (+) 564 WP_116546775.1 YqgE/AlgH family protein -
  LPH35_RS02850 ruvX 627871..628290 (+) 420 WP_010864551.1 Holliday junction resolvase RuvX -
  LPH35_RS02855 npr 628307..628582 (-) 276 WP_047708160.1 PTS phosphocarrier protein NPr -
  LPH35_RS02860 pilU 628820..629929 (-) 1110 WP_230432757.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LPH35_RS02865 pilT 629913..631013 (-) 1101 WP_152109238.1 type IV pilus twitching motility protein PilT Machinery gene
  LPH35_RS02870 - 631036..631743 (+) 708 WP_230432758.1 YggS family pyridoxal phosphate-dependent enzyme -
  LPH35_RS02875 proC 631826..632644 (+) 819 WP_230432759.1 pyrroline-5-carboxylate reductase -
  LPH35_RS02880 - 632673..633227 (+) 555 WP_010864557.1 YggT family protein -
  LPH35_RS02885 yggU 633239..633526 (+) 288 WP_084976536.1 DUF167 family protein YggU -
  LPH35_RS02890 - 633595..634098 (+) 504 WP_230432760.1 DUF4426 domain-containing protein -
  LPH35_RS02895 - 634278..634883 (+) 606 WP_064978167.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 366 a.a.        Molecular weight: 40435.10 Da        Isoelectric Point: 6.7432

>NTDB_id=631145 LPH35_RS02865 WP_152109238.1 629913..631013(-) (pilT) [Plesiomonas shigelloides strain 7A]
MDITELLAFSVKHNASDLHLSAGNPPLIRVDGEVRRVNMPVLDGREVRRLVYDIMNDVQRRDFETEMELDFSFELNGVGR
FRVNAYQQQRGISAALRTIPTDIPTLEQLNAPEVFRNIAMQPRGLVLVTGPTGSGKSTTLAAMLNYVNQHMHHHILTIED
PIEFIHTSNKCLINQREVHRHTHSFNNALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
VDVFPGAEKDMVRSMLSESLKAVISQVLLRKEGGGRVAAHEIMLGTPAIRNLIREDKIHQIYSILQTSSRQGMQTLEQAL
NVLTSQGVVSYEEAQRKMNSLMDGGIQNGNTAGATGAGAMGYGFAG

Nucleotide


Download         Length: 1101 bp        

>NTDB_id=631145 LPH35_RS02865 WP_152109238.1 629913..631013(-) (pilT) [Plesiomonas shigelloides strain 7A]
ATGGACATAACAGAACTGTTGGCTTTTAGTGTAAAACATAATGCCTCGGATCTGCACCTATCTGCCGGTAACCCACCGCT
GATCCGCGTCGACGGGGAAGTGCGTCGGGTCAATATGCCGGTCCTGGATGGCCGTGAAGTGCGCCGTCTGGTGTATGACA
TTATGAACGATGTTCAGCGCCGTGACTTTGAAACTGAGATGGAACTGGACTTTTCGTTCGAACTGAACGGGGTTGGCCGT
TTCCGGGTTAATGCTTATCAGCAGCAACGGGGAATTTCGGCGGCACTGCGTACCATACCTACAGATATACCAACACTGGA
GCAGTTAAACGCACCGGAAGTGTTTCGGAATATTGCCATGCAGCCGCGTGGACTGGTATTGGTCACCGGCCCGACCGGCT
CGGGGAAGTCGACCACCTTGGCGGCGATGCTCAATTATGTCAATCAGCACATGCATCACCACATTCTGACCATTGAAGAT
CCGATCGAATTTATCCACACCAGTAACAAGTGCCTGATTAACCAGCGTGAGGTGCATCGGCATACCCACAGTTTTAATAA
TGCACTGCGCTCGGCGCTGCGTGAAGACCCCGATGTGATTCTGGTGGGTGAGCTGCGCGATCTGGAAACCATTCGTCTGG
CACTGACTGCCGCCGAAACCGGCCACTTGGTGTTTGGCACCCTGCACACCAGCTCGGCGGCCAAAACCATTGACCGTATT
GTGGATGTGTTCCCCGGCGCGGAAAAAGACATGGTGCGCTCGATGCTGTCTGAATCGCTCAAAGCGGTTATCTCGCAGGT
ATTGCTGCGTAAAGAAGGCGGCGGACGTGTGGCGGCGCACGAGATCATGCTGGGAACGCCAGCGATTCGTAACTTGATCC
GTGAAGATAAAATTCACCAGATCTATTCGATTTTGCAGACCAGCTCGCGCCAAGGTATGCAGACGCTGGAGCAGGCGCTG
AATGTGCTGACCAGTCAGGGCGTGGTCAGCTACGAAGAGGCGCAGCGCAAGATGAACTCGTTGATGGATGGCGGGATCCA
AAACGGCAATACCGCGGGAGCTACCGGTGCAGGAGCGATGGGCTATGGTTTCGCTGGATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

73.373

92.35

0.678
  pilT Vibrio cholerae strain A1552

73.373

92.35

0.678
  pilT Pseudomonas stutzeri DSM 10701

71.217

92.077

0.656
  pilT Acinetobacter nosocomialis M2

71.343

91.53

0.653
  pilT Acinetobacter baumannii D1279779

71.045

91.53

0.65
  pilT Acinetobacter baumannii strain A118

71.045

91.53

0.65
  pilT Pseudomonas aeruginosa PAK

70.623

92.077

0.65
  pilT Acinetobacter baylyi ADP1

70.448

91.53

0.645
  pilT Neisseria meningitidis 8013

64.793

92.35

0.598
  pilT Neisseria gonorrhoeae MS11

64.497

92.35

0.596
  pilT Legionella pneumophila strain Lp02

65.269

91.257

0.596
  pilT Legionella pneumophila strain ERS1305867

65.269

91.257

0.596
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.025

98.087

0.481
  pilU Vibrio cholerae strain A1552

43.023

93.989

0.404
  pilU Pseudomonas stutzeri DSM 10701

43.284

91.53

0.396
  pilU Acinetobacter baylyi ADP1

42.274

93.716

0.396