Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   LPH35_RS02860 Genome accession   NZ_CP087711
Coordinates   628820..629929 (-) Length   369 a.a.
NCBI ID   WP_230432757.1    Uniprot ID   -
Organism   Plesiomonas shigelloides strain 7A     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 623820..634929
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LPH35_RS02825 - 624472..624681 (-) 210 WP_010864545.1 DUF1656 domain-containing protein -
  LPH35_RS02830 - 624678..625190 (-) 513 WP_010864546.1 MarR family winged helix-turn-helix transcriptional regulator -
  LPH35_RS02835 rsmE 625372..626106 (+) 735 WP_010864547.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  LPH35_RS02840 gshB 626116..627069 (+) 954 WP_047708158.1 glutathione synthase -
  LPH35_RS02845 - 627283..627846 (+) 564 WP_116546775.1 YqgE/AlgH family protein -
  LPH35_RS02850 ruvX 627871..628290 (+) 420 WP_010864551.1 Holliday junction resolvase RuvX -
  LPH35_RS02855 npr 628307..628582 (-) 276 WP_047708160.1 PTS phosphocarrier protein NPr -
  LPH35_RS02860 pilU 628820..629929 (-) 1110 WP_230432757.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LPH35_RS02865 pilT 629913..631013 (-) 1101 WP_152109238.1 type IV pilus twitching motility protein PilT Machinery gene
  LPH35_RS02870 - 631036..631743 (+) 708 WP_230432758.1 YggS family pyridoxal phosphate-dependent enzyme -
  LPH35_RS02875 proC 631826..632644 (+) 819 WP_230432759.1 pyrroline-5-carboxylate reductase -
  LPH35_RS02880 - 632673..633227 (+) 555 WP_010864557.1 YggT family protein -
  LPH35_RS02885 yggU 633239..633526 (+) 288 WP_084976536.1 DUF167 family protein YggU -
  LPH35_RS02890 - 633595..634098 (+) 504 WP_230432760.1 DUF4426 domain-containing protein -
  LPH35_RS02895 - 634278..634883 (+) 606 WP_064978167.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41533.60 Da        Isoelectric Point: 5.8959

>NTDB_id=631144 LPH35_RS02860 WP_230432757.1 628820..629929(-) (pilU) [Plesiomonas shigelloides strain 7A]
MVSLDELLQEMLRQQASDLYVSVDVPAQLRINHELHPLGEALSIETVTELVAQAMGAERFLQFCQQKEGNMALVRPFARF
RVSGFWQRELPGMVIRHIRTYIPTIEELGLPMLTGELAMTRKGLILVVGATGSGKTTTLAAMLGYRNEFDHSHILTVEDP
IEFIHPHKRCLITQREVGMDTESYEVALRNALRQAPDVIQVGEIRTRDTLQHAIGFSETGHLCIATLHANNASQALERMV
HMIPEGSREAFLFDLSNNLRAIIGQQLVPGITPGRRLLVHEILINTPRVTDLLRKNQLYELRDVMASNSEIGMCTFDQSL
YGLYRRGLISYTDALHYAESANDLRLRIRSSGDEPFSPNTFDDVTVDLS

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=631144 LPH35_RS02860 WP_230432757.1 628820..629929(-) (pilU) [Plesiomonas shigelloides strain 7A]
ATGGTTTCGCTGGATGAGTTATTGCAGGAGATGCTGCGCCAGCAGGCGTCGGATCTGTATGTTTCGGTCGATGTGCCCGC
GCAGCTGCGCATCAATCATGAGTTGCACCCACTCGGTGAGGCGCTGTCGATTGAAACGGTTACCGAGCTGGTGGCGCAGG
CGATGGGCGCCGAGCGCTTCTTACAGTTTTGTCAGCAAAAAGAGGGCAACATGGCGCTGGTGCGTCCGTTTGCCCGTTTT
CGGGTTTCCGGCTTTTGGCAGCGCGAATTGCCGGGTATGGTGATCCGTCATATTCGTACCTATATTCCGACCATTGAAGA
GCTGGGCTTACCGATGTTAACCGGTGAGCTGGCGATGACGCGCAAAGGTTTGATTTTGGTGGTCGGCGCTACCGGCAGCG
GGAAAACCACCACGCTGGCAGCAATGCTCGGCTATCGCAACGAGTTTGACCATTCGCATATTCTGACGGTGGAAGACCCG
ATTGAATTTATCCATCCGCATAAACGCTGTTTGATCACTCAGCGTGAAGTGGGGATGGATACCGAAAGCTACGAGGTAGC
CTTGCGTAACGCGCTGCGCCAAGCTCCGGATGTGATTCAGGTCGGTGAGATCCGCACCCGCGATACGCTGCAACACGCGA
TTGGCTTTTCCGAAACCGGCCATCTGTGTATCGCCACCTTACACGCCAATAACGCCAGCCAAGCACTGGAGCGAATGGTT
CACATGATCCCTGAAGGCAGCCGCGAAGCTTTCTTGTTTGATTTATCCAACAACTTGCGCGCCATTATCGGTCAGCAGCT
AGTGCCGGGGATCACCCCTGGACGCCGTTTGCTGGTGCACGAGATTTTAATCAACACGCCGCGCGTGACCGATTTGCTGC
GTAAAAATCAGTTGTATGAGCTGCGTGACGTAATGGCCAGTAACAGCGAAATTGGCATGTGCACCTTTGACCAATCGCTG
TATGGCTTGTATCGGCGCGGGCTGATTAGCTATACCGATGCGCTGCACTATGCGGAGTCAGCTAACGATTTGCGTTTGCG
GATCCGCAGTAGCGGGGATGAGCCGTTCTCACCGAACACCTTCGATGATGTGACGGTGGATTTAAGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(119-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

54.768

99.458

0.545
  pilU Pseudomonas stutzeri DSM 10701

51.275

95.664

0.491
  pilU Acinetobacter baylyi ADP1

46.742

95.664

0.447
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.344

99.187

0.39