Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   IUZ65_RS02050 Genome accession   NZ_CP087590
Coordinates   414294..415337 (+) Length   347 a.a.
NCBI ID   WP_195702142.1    Uniprot ID   -
Organism   Vibrio sp. VB16     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 409294..420337
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IUZ65_RS02020 (IUZ65_002020) hemW 410016..411164 (-) 1149 WP_195704973.1 radical SAM family heme chaperone HemW -
  IUZ65_RS02025 (IUZ65_002025) - 411189..411788 (-) 600 WP_195702137.1 XTP/dITP diphosphatase -
  IUZ65_RS02030 (IUZ65_002030) yggU 411827..412114 (-) 288 WP_195702138.1 DUF167 family protein YggU -
  IUZ65_RS02035 (IUZ65_002035) - 412114..412668 (-) 555 WP_195702139.1 YggT family protein -
  IUZ65_RS02040 (IUZ65_002040) proC 412710..413528 (-) 819 WP_195702140.1 pyrroline-5-carboxylate reductase -
  IUZ65_RS02045 (IUZ65_002045) - 413559..414269 (-) 711 WP_195702141.1 YggS family pyridoxal phosphate-dependent enzyme -
  IUZ65_RS02050 (IUZ65_002050) pilT 414294..415337 (+) 1044 WP_195702142.1 type IV pilus twitching motility protein PilT Machinery gene
  IUZ65_RS02055 (IUZ65_002055) pilU 415342..416463 (+) 1122 WP_195702143.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  IUZ65_RS02060 (IUZ65_002060) ruvX 416513..416944 (-) 432 WP_195702144.1 Holliday junction resolvase RuvX -
  IUZ65_RS02065 (IUZ65_002065) - 417006..417569 (-) 564 WP_195702145.1 YqgE/AlgH family protein -
  IUZ65_RS02070 (IUZ65_002070) gshB 417588..418535 (-) 948 WP_195702146.1 glutathione synthase -
  IUZ65_RS02075 (IUZ65_002075) rsmE 418570..419301 (-) 732 WP_195702147.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  IUZ65_RS02080 (IUZ65_002080) - 419420..419920 (-) 501 WP_195702148.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38514.40 Da        Isoelectric Point: 6.3180

>NTDB_id=630214 IUZ65_RS02050 WP_195702142.1 414294..415337(+) (pilT) [Vibrio sp. VB16]
MDITELLDFSVKHNASDLHLSAGVAPMIRIDGDVRKLGIPPLSHSEVHHLLFEIMTDAQRSEFEEKLEVDFSYEIPQIGR
FRINAFNQRRGCSAVLRTIPMDIPTLEELDAPSIFSEISEMEKGLILVTGPTGSGKSTTLAAMVNHINRNFNKHILTIED
PIEFVHSNIKCLINQREVHKDTHSFKAALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKSMVRSMLSESLKTVIAQKLLKKIGGGRVACHEIMMGTPAIRNLIREDKVAQMYSIIQTSSSVGMRTMEQSA
KQLLAKNIVDIDEVNKKIEMEAMSFGS

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=630214 IUZ65_RS02050 WP_195702142.1 414294..415337(+) (pilT) [Vibrio sp. VB16]
ATGGATATCACTGAGCTATTAGATTTTAGTGTAAAACATAATGCCTCAGATCTACATCTTTCTGCTGGTGTTGCCCCTAT
GATACGCATTGATGGCGATGTTAGGAAGCTAGGTATTCCTCCTTTAAGTCATTCTGAGGTGCATCACCTGTTATTTGAAA
TAATGACGGATGCACAAAGAAGTGAATTTGAAGAGAAGCTTGAAGTCGATTTTTCGTATGAGATTCCACAAATAGGCCGA
TTCCGCATCAATGCTTTTAATCAAAGACGTGGGTGCTCAGCTGTTTTGCGCACCATCCCAATGGATATCCCTACGCTAGA
AGAGTTAGATGCACCAAGTATATTTAGTGAAATTTCCGAAATGGAAAAAGGCTTGATATTGGTCACTGGGCCAACGGGTT
CTGGAAAATCAACAACGTTAGCGGCAATGGTTAATCATATCAATCGTAACTTTAATAAGCATATATTAACCATCGAAGAC
CCAATTGAATTTGTGCATTCAAATATTAAGTGTTTAATTAATCAACGTGAGGTGCATAAAGACACCCATAGCTTTAAGGC
CGCGTTAAGGTCGGCACTGCGTGAAGACCCAGATGTCATTCTTGTTGGTGAATTAAGGGACCAAGAAACCATTAGTTTGG
CGTTGACGGCTGCAGAAACGGGTCATTTGGTATTTGGTACTTTGCATACAAGCTCAGCGGCAAAAACGATTGATCGAATT
ATTGATGTTTTCCCAGGAAGTGACAAATCGATGGTTCGGTCAATGTTGTCTGAATCTCTTAAGACCGTTATTGCACAAAA
ATTATTGAAAAAAATTGGTGGTGGCCGAGTTGCATGTCACGAAATTATGATGGGCACACCCGCAATCCGAAATCTTATCC
GAGAAGATAAAGTTGCCCAAATGTATTCCATTATTCAAACGAGCTCTTCCGTTGGTATGAGAACAATGGAACAGAGTGCG
AAGCAGCTGTTGGCGAAGAATATCGTCGACATTGATGAAGTAAATAAGAAAATTGAGATGGAGGCGATGTCGTTTGGGTC
ATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

81.159

99.424

0.807
  pilT Vibrio cholerae strain A1552

81.159

99.424

0.807
  pilT Acinetobacter baylyi ADP1

69.486

95.389

0.663
  pilT Pseudomonas aeruginosa PAK

66.765

97.983

0.654
  pilT Pseudomonas stutzeri DSM 10701

67.062

97.118

0.651
  pilT Acinetobacter baumannii D1279779

68.389

94.813

0.648
  pilT Acinetobacter baumannii strain A118

68.389

94.813

0.648
  pilT Acinetobacter nosocomialis M2

68.085

94.813

0.646
  pilT Legionella pneumophila strain Lp02

67.173

94.813

0.637
  pilT Legionella pneumophila strain ERS1305867

67.173

94.813

0.637
  pilT Neisseria meningitidis 8013

60.231

100

0.602
  pilT Neisseria gonorrhoeae MS11

59.942

100

0.599
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.102

96.254

0.473
  pilU Vibrio cholerae strain A1552

42.462

93.66

0.398
  pilU Pseudomonas stutzeri DSM 10701

40.367

94.236

0.38
  pilU Acinetobacter baylyi ADP1

40.81

92.507

0.378