Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   IUZ65_RS02055 Genome accession   NZ_CP087590
Coordinates   415342..416463 (+) Length   373 a.a.
NCBI ID   WP_195702143.1    Uniprot ID   -
Organism   Vibrio sp. VB16     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 410342..421463
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IUZ65_RS02025 (IUZ65_002025) - 411189..411788 (-) 600 WP_195702137.1 XTP/dITP diphosphatase -
  IUZ65_RS02030 (IUZ65_002030) yggU 411827..412114 (-) 288 WP_195702138.1 DUF167 family protein YggU -
  IUZ65_RS02035 (IUZ65_002035) - 412114..412668 (-) 555 WP_195702139.1 YggT family protein -
  IUZ65_RS02040 (IUZ65_002040) proC 412710..413528 (-) 819 WP_195702140.1 pyrroline-5-carboxylate reductase -
  IUZ65_RS02045 (IUZ65_002045) - 413559..414269 (-) 711 WP_195702141.1 YggS family pyridoxal phosphate-dependent enzyme -
  IUZ65_RS02050 (IUZ65_002050) pilT 414294..415337 (+) 1044 WP_195702142.1 type IV pilus twitching motility protein PilT Machinery gene
  IUZ65_RS02055 (IUZ65_002055) pilU 415342..416463 (+) 1122 WP_195702143.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  IUZ65_RS02060 (IUZ65_002060) ruvX 416513..416944 (-) 432 WP_195702144.1 Holliday junction resolvase RuvX -
  IUZ65_RS02065 (IUZ65_002065) - 417006..417569 (-) 564 WP_195702145.1 YqgE/AlgH family protein -
  IUZ65_RS02070 (IUZ65_002070) gshB 417588..418535 (-) 948 WP_195702146.1 glutathione synthase -
  IUZ65_RS02075 (IUZ65_002075) rsmE 418570..419301 (-) 732 WP_195702147.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  IUZ65_RS02080 (IUZ65_002080) - 419420..419920 (-) 501 WP_195702148.1 SprT family zinc-dependent metalloprotease -
  IUZ65_RS02085 (IUZ65_002085) - 419980..420774 (-) 795 WP_195702149.1 DUF2189 domain-containing protein -

Sequence

Protein


Download         Length: 373 a.a.        Molecular weight: 41669.69 Da        Isoelectric Point: 5.6487

>NTDB_id=630215 IUZ65_RS02055 WP_195702143.1 415342..416463(+) (pilU) [Vibrio sp. VB16]
MDLNKAVDINTLLYEMSVNKASDIYITVDSPCLLRIDGQLRAIGEKLNKESVSFLLNALMDDEKKSEFDRTKEANFAIVR
NSGRYRVSAFFQRELPGAVIRRIETEIPTCDELMLPEIMKELSVAKRGLVLIVGSTGSGKSTTMAAMTGYRNQNRSGHIL
TVEDPIEFVHEHDQCIVTQREVGVDTESYEVALKNSLRQAPDMILIGEIRSRETMEFAMTFAETGHLCMATLHANNANQA
LERILNLVPKDHKEQFLFDLSSNLKGIIGQQLIIDKHGQGRHGVFEVLLNTPRVSDLIRRGDLHELKPTMVKSAESGMRT
FDQSLYQLVVEGKITEVDALHSADSANDLRLMMKTNRGELDSSALSGVKVDMG

Nucleotide


Download         Length: 1122 bp        

>NTDB_id=630215 IUZ65_RS02055 WP_195702143.1 415342..416463(+) (pilU) [Vibrio sp. VB16]
ATGGATCTTAACAAGGCTGTTGATATTAATACTCTTCTGTATGAGATGAGCGTCAATAAAGCGTCAGATATATACATTAC
GGTCGACTCGCCTTGCCTGCTGCGTATTGACGGGCAATTGCGTGCCATCGGAGAAAAACTGAATAAAGAGAGCGTCTCTT
TTTTGCTTAATGCTCTGATGGATGATGAAAAAAAATCGGAATTTGATCGAACCAAAGAAGCGAACTTTGCGATAGTACGC
AATAGTGGCCGATATCGTGTGAGTGCTTTTTTTCAACGAGAGCTGCCTGGTGCTGTGATAAGAAGAATCGAGACAGAAAT
CCCTACCTGTGATGAATTGATGCTCCCAGAGATAATGAAAGAGCTTTCCGTTGCAAAAAGAGGACTCGTTTTGATTGTCG
GTTCTACTGGTTCAGGGAAATCGACCACCATGGCAGCAATGACTGGATATCGAAATCAGAACAGAAGCGGTCATATTTTG
ACAGTGGAAGACCCAATTGAATTTGTTCATGAACATGACCAATGCATTGTTACGCAAAGGGAGGTTGGTGTTGATACTGA
AAGCTATGAAGTGGCATTAAAAAACTCACTTCGCCAAGCACCAGATATGATCTTAATTGGTGAGATTCGTAGTCGTGAAA
CTATGGAATTTGCCATGACATTTGCCGAAACAGGCCATCTATGTATGGCGACATTGCATGCAAACAATGCGAATCAAGCG
TTAGAACGCATTCTTAATCTGGTTCCTAAAGACCATAAAGAACAGTTTTTATTTGACCTATCGAGCAACTTGAAGGGCAT
AATTGGTCAACAGTTGATTATAGATAAACATGGACAAGGCCGACATGGTGTTTTTGAGGTATTACTTAATACACCAAGAG
TTTCCGATCTTATTCGGCGCGGTGATCTTCATGAACTGAAACCAACAATGGTTAAATCAGCAGAATCAGGTATGAGGACA
TTTGACCAGTCGCTTTACCAACTTGTGGTAGAAGGCAAGATCACAGAAGTTGATGCACTACACAGTGCTGATTCTGCAAA
TGATTTGCGACTAATGATGAAGACAAATAGGGGCGAACTCGACAGTAGTGCATTGTCAGGTGTAAAGGTAGATATGGGGT
AA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(16-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

74.659

98.391

0.735
  pilU Pseudomonas stutzeri DSM 10701

54.178

99.464

0.539
  pilU Acinetobacter baylyi ADP1

52.991

94.102

0.499
  pilT Legionella pneumophila strain Lp02

40.708

90.885

0.37
  pilT Legionella pneumophila strain ERS1305867

40.708

90.885

0.37
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.942

91.957

0.367
  pilT Vibrio cholerae strain A1552

41.088

88.74

0.365
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.088

88.74

0.365
  pilT Acinetobacter baumannii D1279779

40.118

90.885

0.365
  pilT Acinetobacter baumannii strain A118

40.118

90.885

0.365
  pilT Acinetobacter nosocomialis M2

40.118

90.885

0.365
  pilT Pseudomonas aeruginosa PAK

39.823

90.885

0.362
  pilT Acinetobacter baylyi ADP1

40.909

88.472

0.362