Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CENROD_RS10170 Genome accession   NC_022576
Coordinates   2491452..2492495 (-) Length   347 a.a.
NCBI ID   WP_022775753.1    Uniprot ID   -
Organism   Candidatus Symbiobacter mobilis CR     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2486452..2497495
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CENROD_RS10150 (Cenrod_2264) - 2487224..2488153 (+) 930 WP_022775739.1 branched-chain amino acid ABC transporter permease -
  CENROD_RS10155 (Cenrod_2266) - 2488150..2489511 (+) 1362 WP_081699880.1 branched-chain amino acid ABC transporter permease -
  CENROD_RS10160 (Cenrod_2267) - 2489448..2490251 (+) 804 WP_151194619.1 ABC transporter ATP-binding protein -
  CENROD_RS10165 (Cenrod_2268) pilU 2490264..2491400 (-) 1137 WP_022775750.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  CENROD_RS10170 (Cenrod_2269) pilT 2491452..2492495 (-) 1044 WP_022775753.1 type IV pilus twitching motility protein PilT Machinery gene
  CENROD_RS10175 (Cenrod_2270) - 2492556..2492762 (+) 207 WP_022775756.1 Trm112 family protein -
  CENROD_RS10180 (Cenrod_2271) kdsB 2492898..2493692 (+) 795 WP_022775760.1 3-deoxy-manno-octulosonate cytidylyltransferase -
  CENROD_RS10185 (Cenrod_2273) gspK 2493773..2494828 (-) 1056 WP_022775763.1 type II secretion system minor pseudopilin GspK -
  CENROD_RS10190 (Cenrod_2274) - 2494828..2495514 (-) 687 WP_022775767.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  CENROD_RS10195 (Cenrod_2275) gspI 2495570..2495944 (-) 375 WP_022775771.1 type II secretion system minor pseudopilin GspI -
  CENROD_RS10200 (Cenrod_2276) - 2495941..2496483 (-) 543 WP_081699954.1 GspH/FimT family pseudopilin -
  CENROD_RS10205 (Cenrod_2277) gspG 2496597..2497034 (-) 438 WP_022775779.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38389.14 Da        Isoelectric Point: 6.8315

>NTDB_id=62868 CENROD_RS10170 WP_022775753.1 2491452..2492495(-) (pilT) [Candidatus Symbiobacter mobilis CR]
MDITQLLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINVDLLDHKQVHAMVYDIMNDSQRKTYEEYMEIDFSFEIEGLAR
FRVNAFNQNRGAGAVFRTIPSKILTLEQLNCPKIFSELALRPRGLVLVTGPTGSGKSTTLAAMVNYLNENEYGHILTVED
PIEFVHDSKKCLINQREVGPHTMSFAAALKSALREDPDAILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAEEKEMVRAMLSESLQAVISQTLCKTKDGQGRVAAHEIMLGTSAIRNLIREAKVAQMYSSIQTGNSVGMQTLDQN
LTDLVRRNVIAPAEARSKAKIPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=62868 CENROD_RS10170 WP_022775753.1 2491452..2492495(-) (pilT) [Candidatus Symbiobacter mobilis CR]
ATGGACATTACGCAGTTGCTGGCATTCAGCGTCAAGAACAAGGCTTCGGACTTGCACCTGTCCGCCGGGCTGCCGCCCAT
GATCCGCGTACATGGCGACGTGCGCCGAATCAATGTCGACTTGCTGGACCATAAGCAAGTCCACGCGATGGTCTACGACA
TCATGAACGACTCGCAGCGCAAGACCTACGAGGAATACATGGAAATCGACTTCTCGTTCGAAATCGAAGGGCTGGCGCGG
TTCCGGGTCAACGCCTTCAACCAGAACCGGGGGGCAGGCGCGGTGTTTCGGACGATTCCCAGCAAGATCCTCACGCTCGA
ACAGCTCAACTGCCCCAAGATCTTTTCCGAGCTGGCGCTGCGCCCGCGCGGGTTGGTCCTGGTTACAGGCCCGACGGGGT
CGGGCAAGTCCACCACCTTGGCGGCGATGGTGAACTACCTCAATGAAAACGAATACGGCCACATCCTCACGGTCGAAGAC
CCCATCGAATTCGTACACGATTCCAAAAAGTGCCTGATCAACCAGCGCGAAGTCGGCCCGCACACGATGAGCTTTGCCGC
TGCGCTCAAATCCGCCCTGCGCGAAGACCCCGACGCCATCCTCGTTGGCGAAATGCGCGACCTCGAAACGATCCGCTTGG
CCATGACCGCCGCCGAGACAGGGCATCTGGTGTTCGGCACGCTGCATACCTCCAGCGCCGCCAAGACCATCGACCGGATC
ATCGACGTCTTCCCCGCCGAAGAAAAGGAAATGGTTCGTGCCATGTTGTCCGAATCCCTGCAAGCGGTCATCTCCCAAAC
CCTGTGCAAGACCAAGGATGGCCAAGGGCGCGTCGCGGCGCACGAAATCATGCTGGGAACGAGCGCGATCCGCAACCTGA
TCCGCGAGGCCAAGGTTGCGCAGATGTACTCCAGTATCCAGACCGGCAATAGCGTAGGCATGCAGACGCTCGACCAAAAC
CTGACCGACCTCGTCCGGCGCAATGTCATCGCCCCGGCCGAGGCACGCTCGAAGGCCAAAATTCCCGAGAATTTCCCGGG
CTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

76.812

99.424

0.764
  pilT Acinetobacter baumannii strain A118

76.812

99.424

0.764
  pilT Acinetobacter nosocomialis M2

76.812

99.424

0.764
  pilT Pseudomonas aeruginosa PAK

76.232

99.424

0.758
  pilT Pseudomonas stutzeri DSM 10701

75.652

99.424

0.752
  pilT Acinetobacter baylyi ADP1

74.783

99.424

0.744
  pilT Legionella pneumophila strain Lp02

73.451

97.695

0.718
  pilT Legionella pneumophila strain ERS1305867

73.451

97.695

0.718
  pilT Neisseria gonorrhoeae MS11

67.536

99.424

0.671
  pilT Neisseria meningitidis 8013

67.536

99.424

0.671
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.449

98.271

0.663
  pilT Vibrio cholerae strain A1552

67.449

98.271

0.663
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.435

99.424

0.501
  pilU Vibrio cholerae strain A1552

42.985

96.542

0.415
  pilU Acinetobacter baylyi ADP1

41.329

99.712

0.412
  pilU Pseudomonas stutzeri DSM 10701

42.388

96.542

0.409

Multiple sequence alignment