Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   CENROD_RS10165 Genome accession   NC_022576
Coordinates   2490264..2491400 (-) Length   378 a.a.
NCBI ID   WP_022775750.1    Uniprot ID   U5N9X5
Organism   Candidatus Symbiobacter mobilis CR     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2485264..2496400
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CENROD_RS10150 (Cenrod_2264) - 2487224..2488153 (+) 930 WP_022775739.1 branched-chain amino acid ABC transporter permease -
  CENROD_RS10155 (Cenrod_2266) - 2488150..2489511 (+) 1362 WP_081699880.1 branched-chain amino acid ABC transporter permease -
  CENROD_RS10160 (Cenrod_2267) - 2489448..2490251 (+) 804 WP_151194619.1 ABC transporter ATP-binding protein -
  CENROD_RS10165 (Cenrod_2268) pilU 2490264..2491400 (-) 1137 WP_022775750.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  CENROD_RS10170 (Cenrod_2269) pilT 2491452..2492495 (-) 1044 WP_022775753.1 type IV pilus twitching motility protein PilT Machinery gene
  CENROD_RS10175 (Cenrod_2270) - 2492556..2492762 (+) 207 WP_022775756.1 Trm112 family protein -
  CENROD_RS10180 (Cenrod_2271) kdsB 2492898..2493692 (+) 795 WP_022775760.1 3-deoxy-manno-octulosonate cytidylyltransferase -
  CENROD_RS10185 (Cenrod_2273) gspK 2493773..2494828 (-) 1056 WP_022775763.1 type II secretion system minor pseudopilin GspK -
  CENROD_RS10190 (Cenrod_2274) - 2494828..2495514 (-) 687 WP_022775767.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  CENROD_RS10195 (Cenrod_2275) gspI 2495570..2495944 (-) 375 WP_022775771.1 type II secretion system minor pseudopilin GspI -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41975.43 Da        Isoelectric Point: 7.4174

>NTDB_id=62867 CENROD_RS10165 WP_022775750.1 2490264..2491400(-) (pilU) [Candidatus Symbiobacter mobilis CR]
MERDQASTFITDLLKLMLGRGGSDLFLTADFPPAIKVDGKVTKVSPQPLNGGHTVALARSIMNDKQVAEFERTKECNFAI
APPGLGRFRVNAFMQQNKVGMVLRTIPTTLPSIDTLKLPPILKDIVNTKRGLCIMVGATGSGKSTTLAAMVDWRNETTYG
HIITIEDPVEFVHPHKNCVITQREVGIDTESWGAALKNTLRQAPDVILMGEIRDRETMEHAVTFAETGHMCLATLHANSA
NQALDRVINFFPEERRTQLLMDLSLNLRAMVSQRLVPRQDGKGRVAIIEIMLNSPLVSDLIFKGQVAEIKEVMKKSRNIG
MQTFDQALFDAYESNLITYEDALRNADSVNDLRLQIKLNSQRGRSQDLSAGTETMNII

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=62867 CENROD_RS10165 WP_022775750.1 2490264..2491400(-) (pilU) [Candidatus Symbiobacter mobilis CR]
ATGGAACGCGACCAGGCCAGTACGTTTATCACCGATCTGCTCAAGCTGATGCTGGGGCGCGGGGGCAGTGACCTGTTCCT
CACCGCAGACTTTCCGCCTGCCATCAAAGTCGATGGCAAAGTCACCAAGGTATCACCACAGCCGCTCAACGGCGGGCATA
CCGTCGCGCTCGCGCGGTCGATCATGAACGACAAGCAGGTTGCGGAGTTCGAGCGCACCAAGGAATGCAACTTCGCCATC
GCCCCGCCCGGGCTGGGACGCTTTCGCGTCAACGCCTTCATGCAGCAAAACAAGGTTGGCATGGTGCTGCGGACGATCCC
GACGACGCTGCCTTCGATCGACACGTTGAAGCTTCCGCCCATTCTCAAAGACATCGTCAACACCAAGCGTGGCTTGTGCA
TCATGGTCGGCGCAACGGGGTCGGGCAAATCGACGACGCTGGCCGCGATGGTCGACTGGCGCAACGAAACGACCTATGGA
CACATCATCACGATCGAGGATCCGGTCGAATTCGTCCACCCGCACAAAAACTGCGTCATCACCCAGCGTGAAGTCGGCAT
CGATACCGAAAGCTGGGGCGCAGCGCTCAAAAACACGCTGCGGCAGGCCCCGGACGTGATTCTGATGGGGGAAATTCGCG
ACCGGGAAACCATGGAACACGCTGTCACCTTTGCGGAAACCGGCCACATGTGCCTAGCCACGCTCCACGCCAACAGCGCC
AACCAGGCACTCGACCGCGTCATCAACTTCTTCCCTGAAGAGCGCCGGACGCAATTGCTGATGGATCTGTCGCTGAACCT
GCGTGCCATGGTGTCGCAGCGGCTCGTTCCCCGGCAAGACGGCAAGGGCCGGGTGGCGATCATCGAAATCATGCTCAATT
CCCCACTCGTCTCCGACCTCATCTTCAAAGGCCAGGTCGCCGAAATCAAGGAAGTGATGAAAAAGAGCCGCAACATCGGC
ATGCAGACCTTTGACCAAGCGCTGTTCGACGCCTACGAGAGCAACCTCATCACCTATGAAGATGCCTTGCGCAACGCCGA
TTCGGTCAATGATCTGCGCTTGCAGATCAAGCTCAACAGCCAGCGCGGCCGCTCGCAGGATCTGAGCGCCGGCACGGAGA
CGATGAACATCATTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(120-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB U5N9X5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

62.178

92.328

0.574
  pilU Acinetobacter baylyi ADP1

55.71

94.974

0.529
  pilU Vibrio cholerae strain A1552

51.404

94.18

0.484
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.071

89.153

0.429
  pilT Pseudomonas aeruginosa PAK

44.51

89.153

0.397
  pilT Acinetobacter baumannii D1279779

46.082

84.392

0.389
  pilT Acinetobacter baumannii strain A118

46.082

84.392

0.389
  pilT Acinetobacter nosocomialis M2

46.082

84.392

0.389
  pilT Pseudomonas stutzeri DSM 10701

43.62

89.153

0.389
  pilT Legionella pneumophila strain Lp02

43.243

88.095

0.381
  pilT Legionella pneumophila strain ERS1305867

43.243

88.095

0.381
  pilT Acinetobacter baylyi ADP1

44.828

84.392

0.378
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

44.795

83.862

0.376
  pilT Vibrio cholerae strain A1552

44.795

83.862

0.376
  pilT Neisseria gonorrhoeae MS11

41.543

89.153

0.37
  pilT Neisseria meningitidis 8013

41.543

89.153

0.37

Multiple sequence alignment