Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   KXU59_RS20770 Genome accession   NZ_CP087266
Coordinates   4403550..4404584 (-) Length   344 a.a.
NCBI ID   WP_005340354.1    Uniprot ID   -
Organism   Aeromonas veronii strain HD6448     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4398550..4409584
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KXU59_RS20740 (LOS88_20740) glnK 4399142..4399480 (-) 339 WP_005341406.1 P-II family nitrogen regulator -
  KXU59_RS20745 (LOS88_20745) arfB 4399681..4400097 (+) 417 WP_167564868.1 alternative ribosome rescue aminoacyl-tRNA hydrolase ArfB -
  KXU59_RS20750 (LOS88_20750) - 4400119..4400610 (+) 492 WP_244774639.1 methylated-DNA--[protein]-cysteine S-methyltransferase -
  KXU59_RS20755 (LOS88_20755) - 4400971..4401396 (-) 426 WP_244774637.1 hypothetical protein -
  KXU59_RS20760 (LOS88_20760) yaaA 4401591..4402364 (-) 774 WP_244774635.1 peroxide stress protein YaaA -
  KXU59_RS20765 (LOS88_20765) pilU 4402412..4403521 (-) 1110 WP_005340356.1 type IVa pilus ATPase TapU Machinery gene
  KXU59_RS20770 (LOS88_20770) pilT 4403550..4404584 (-) 1035 WP_005340354.1 type IVa pilus ATPase TapT Machinery gene
  KXU59_RS20775 (LOS88_20775) - 4404624..4405325 (+) 702 WP_013724131.1 YggS family pyridoxal phosphate-dependent enzyme -
  KXU59_RS20780 (LOS88_20780) proC 4405467..4406291 (+) 825 WP_244774633.1 pyrroline-5-carboxylate reductase -
  KXU59_RS20785 (LOS88_20785) - 4406303..4406854 (+) 552 WP_115521283.1 YggT family protein -
  KXU59_RS20790 (LOS88_20790) yggU 4406854..4407156 (+) 303 WP_021230942.1 DUF167 family protein YggU -
  KXU59_RS20795 (LOS88_20795) - 4407200..4407619 (+) 420 WP_005340339.1 DUF4426 domain-containing protein -
  KXU59_RS20800 (LOS88_20800) - 4407721..4408917 (-) 1197 WP_005348874.1 NupC/NupG family nucleoside CNT transporter -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38273.03 Da        Isoelectric Point: 6.7440

>NTDB_id=628270 KXU59_RS20770 WP_005340354.1 4403550..4404584(-) (pilT) [Aeromonas veronii strain HD6448]
MDITELLAFSVKHKASDLHLSAGVPPMIRVDGEVRKINLPALEHREVHALIYDIMNDHQRKELEENFEVDFSFEVPNLAR
FRVNAFQQARGSGAVFRTIPSTVLSLEDLDAPEIFRKIAEFPRGLVLVTGPTGSGKSTTLAAMVNYINENFHHHILTIED
PIEFVHENKRCLVNQREVHRDTKSFSNALRSALREDPDIILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGAEKDMVRSMLSESLRAVISQTLLKRIGGGRVAAHEIMMGIPAVRNLIREDKIAQLYSVIQTGMTHGMQTMDQSL
KQLVSRGVVASLDAKAKAVDPNSI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=628270 KXU59_RS20770 WP_005340354.1 4403550..4404584(-) (pilT) [Aeromonas veronii strain HD6448]
ATGGATATCACAGAGTTATTGGCTTTCAGTGTAAAGCATAAAGCCTCGGATCTACACCTCTCGGCCGGGGTTCCCCCGAT
GATCAGGGTTGATGGTGAGGTTCGCAAGATCAATTTGCCCGCCCTGGAACACCGGGAAGTGCATGCCCTCATTTACGACA
TCATGAACGACCATCAGCGCAAGGAGCTGGAGGAGAACTTCGAGGTCGACTTCTCGTTCGAGGTGCCCAATCTGGCCCGT
TTCCGGGTCAACGCCTTCCAGCAGGCGCGCGGCTCGGGCGCGGTATTTCGTACCATCCCCAGCACTGTGCTGAGCCTCGA
GGATCTCGATGCGCCGGAGATCTTCCGCAAGATCGCCGAGTTCCCGCGGGGTCTGGTGTTGGTGACCGGCCCGACCGGCT
CGGGCAAGTCCACCACACTGGCGGCCATGGTCAACTACATCAACGAGAACTTCCACCACCACATACTCACTATCGAAGAC
CCCATCGAATTCGTACACGAGAACAAACGCTGTCTGGTGAACCAGCGGGAAGTACACCGCGATACCAAGAGCTTCAGCAA
CGCCCTGCGCTCGGCACTGCGGGAAGACCCGGACATCATACTGGTGGGCGAGATGCGCGACCTTGAGACCATTCGTCTGG
CCATGACGGCGGCCGAGACGGGCCACCTGGTGTTTGGCACCCTGCACACCTCGTCAGCGGCCAAGACCATCGACCGTATC
ATCGACGTCTTCCCCGGCGCGGAAAAAGACATGGTACGATCCATGCTCTCTGAATCCCTGCGCGCAGTCATCTCCCAGAC
CCTGCTCAAGCGTATTGGCGGTGGCCGGGTGGCGGCCCACGAGATCATGATGGGCATTCCGGCGGTGCGTAACCTCATTC
GGGAGGACAAGATTGCCCAGCTTTACTCGGTGATCCAGACCGGGATGACCCACGGCATGCAGACCATGGATCAGAGCCTC
AAGCAGTTGGTGAGTCGCGGTGTGGTGGCATCCCTCGATGCCAAGGCCAAGGCCGTCGATCCCAACAGCATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

77.326

100

0.773
  pilT Acinetobacter baylyi ADP1

77.551

99.709

0.773
  pilT Acinetobacter baumannii D1279779

77.035

100

0.77
  pilT Acinetobacter baumannii strain A118

77.035

100

0.77
  pilT Pseudomonas stutzeri DSM 10701

76.163

100

0.762
  pilT Pseudomonas aeruginosa PAK

75.291

100

0.753
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

75.964

97.965

0.744
  pilT Vibrio cholerae strain A1552

75.964

97.965

0.744
  pilT Legionella pneumophila strain Lp02

72.941

98.837

0.721
  pilT Legionella pneumophila strain ERS1305867

72.941

98.837

0.721
  pilT Neisseria meningitidis 8013

69.855

100

0.701
  pilT Neisseria gonorrhoeae MS11

69.565

100

0.698
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.754

99.419

0.515
  pilU Vibrio cholerae strain A1552

42.899

100

0.43
  pilU Pseudomonas stutzeri DSM 10701

40.58

100

0.407
  pilU Acinetobacter baylyi ADP1

40.462

100

0.407