Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   KXU59_RS20765 Genome accession   NZ_CP087266
Coordinates   4402412..4403521 (-) Length   369 a.a.
NCBI ID   WP_005340356.1    Uniprot ID   -
Organism   Aeromonas veronii strain HD6448     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4397412..4408521
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KXU59_RS20735 (LOS88_20735) - 4397876..4398883 (-) 1008 WP_236397434.1 Fe(3+) ABC transporter substrate-binding protein -
  KXU59_RS20740 (LOS88_20740) glnK 4399142..4399480 (-) 339 WP_005341406.1 P-II family nitrogen regulator -
  KXU59_RS20745 (LOS88_20745) arfB 4399681..4400097 (+) 417 WP_167564868.1 alternative ribosome rescue aminoacyl-tRNA hydrolase ArfB -
  KXU59_RS20750 (LOS88_20750) - 4400119..4400610 (+) 492 WP_244774639.1 methylated-DNA--[protein]-cysteine S-methyltransferase -
  KXU59_RS20755 (LOS88_20755) - 4400971..4401396 (-) 426 WP_244774637.1 hypothetical protein -
  KXU59_RS20760 (LOS88_20760) yaaA 4401591..4402364 (-) 774 WP_244774635.1 peroxide stress protein YaaA -
  KXU59_RS20765 (LOS88_20765) pilU 4402412..4403521 (-) 1110 WP_005340356.1 type IVa pilus ATPase TapU Machinery gene
  KXU59_RS20770 (LOS88_20770) pilT 4403550..4404584 (-) 1035 WP_005340354.1 type IVa pilus ATPase TapT Machinery gene
  KXU59_RS20775 (LOS88_20775) - 4404624..4405325 (+) 702 WP_013724131.1 YggS family pyridoxal phosphate-dependent enzyme -
  KXU59_RS20780 (LOS88_20780) proC 4405467..4406291 (+) 825 WP_244774633.1 pyrroline-5-carboxylate reductase -
  KXU59_RS20785 (LOS88_20785) - 4406303..4406854 (+) 552 WP_115521283.1 YggT family protein -
  KXU59_RS20790 (LOS88_20790) yggU 4406854..4407156 (+) 303 WP_021230942.1 DUF167 family protein YggU -
  KXU59_RS20795 (LOS88_20795) - 4407200..4407619 (+) 420 WP_005340339.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41076.12 Da        Isoelectric Point: 6.5043

>NTDB_id=628269 KXU59_RS20765 WP_005340356.1 4402412..4403521(-) (pilU) [Aeromonas veronii strain HD6448]
MNLDDLLSELVERKGSDLFVTVGSPPTLKVNGHLVSLGGEALDKKGALTLVRDTLSSDHFERYIRTKEANYAIYREALGR
FRVSAFWQQELPGMVVRRIETRIPTFEDLQLPKILQEVAMAKRGLVLFVGATGAGKSTTQAAMIGYRNQHADGHILTVED
PVEFVHQHGRSLVTQREVGIDTESFDVALKSSLRQAPDVILIGEIRSQETMEFALQFAETGHLCLATLHANNANQALDRI
LHLVPQDKHRQFLFDLSFNLKAIVAQQLVPSMDGKRRCAAFEILLNTPLITDIIRKGEMHRLKEVMTKSTELGMQTFDQA
LFSLFCAGQIGYSEALAHADSANDLRLLIKLSGRERLGTGTLDNVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=628269 KXU59_RS20765 WP_005340356.1 4402412..4403521(-) (pilU) [Aeromonas veronii strain HD6448]
ATGAATCTGGATGATCTGCTGAGCGAGCTGGTCGAGCGAAAGGGATCGGATCTGTTTGTGACGGTGGGCTCGCCGCCCAC
CCTCAAGGTGAATGGTCATCTGGTGTCGCTGGGGGGGGAAGCGCTCGACAAGAAGGGGGCGCTAACGCTGGTCAGGGATA
CCCTCAGCAGCGATCACTTCGAGCGCTATATCCGAACCAAGGAGGCCAACTACGCGATCTATCGCGAGGCGCTTGGCCGT
TTTCGGGTCAGCGCCTTCTGGCAGCAGGAGCTGCCCGGCATGGTGGTGCGGCGTATCGAGACCCGCATTCCCACCTTTGA
AGACCTGCAGCTACCCAAGATCCTGCAAGAGGTGGCGATGGCCAAGCGGGGGCTGGTGCTGTTTGTCGGCGCCACCGGGG
CGGGCAAATCGACTACCCAGGCGGCGATGATCGGCTATCGCAACCAGCATGCCGATGGTCACATTCTGACGGTGGAAGAT
CCGGTGGAGTTCGTCCATCAGCATGGTCGTAGTCTGGTGACCCAGCGAGAGGTGGGGATCGATACCGAGTCGTTCGATGT
GGCGCTGAAAAGCTCGCTGCGTCAGGCACCGGACGTGATCCTGATCGGCGAGATCCGAAGTCAGGAGACCATGGAGTTTG
CCCTGCAGTTCGCCGAGACGGGACACCTTTGTCTCGCCACTTTGCATGCCAACAATGCCAACCAGGCGCTGGATCGCATC
CTCCATCTGGTGCCGCAAGACAAGCACCGCCAGTTCCTGTTCGATCTCTCTTTCAACCTCAAGGCCATCGTCGCCCAGCA
GCTGGTGCCGAGTATGGATGGCAAGCGGCGCTGCGCTGCGTTCGAGATCCTGCTCAATACCCCGCTCATCACCGACATTA
TCCGCAAGGGAGAGATGCATCGCCTCAAGGAGGTGATGACCAAATCCACCGAGCTGGGTATGCAGACCTTCGATCAAGCG
CTTTTCAGCCTGTTCTGCGCCGGCCAGATTGGCTACAGTGAGGCACTCGCCCACGCTGACTCGGCCAACGACCTGCGACT
GCTGATCAAGCTCTCCGGTCGCGAGCGGCTCGGCACCGGTACGCTGGACAATGTGACGCTGGATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

60.326

99.729

0.602
  pilU Pseudomonas stutzeri DSM 10701

60.399

95.122

0.575
  pilU Acinetobacter baylyi ADP1

57.224

95.664

0.547
  pilT Legionella pneumophila strain Lp02

40.816

92.954

0.379
  pilT Legionella pneumophila strain ERS1305867

40.816

92.954

0.379
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.823

91.87

0.366
  pilT Acinetobacter baylyi ADP1

39.296

92.412

0.363
  pilT Vibrio cholerae strain A1552

39.82

90.515

0.36
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

39.82

90.515

0.36